A Triple-Precursor Blend as a Topical Solution to Protect the Skin Against Environmental Damage
The epidermis acts as the body’s primary defense, relying on components like lipids, HA and GSH for skin barrier function, hydration and resistance to oxidative stress. However, limitations in the topical application of these biomolecules call for novel approaches. This study investigates the effica...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/14/3/266 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849342275298000896 |
|---|---|
| author | Ping Gao Xue Xiao Zhuang Zhou Hong Zhang Raghupathi Subramanian Anuchai Sinsawat Xuelan Gu |
| author_facet | Ping Gao Xue Xiao Zhuang Zhou Hong Zhang Raghupathi Subramanian Anuchai Sinsawat Xuelan Gu |
| author_sort | Ping Gao |
| collection | DOAJ |
| description | The epidermis acts as the body’s primary defense, relying on components like lipids, HA and GSH for skin barrier function, hydration and resistance to oxidative stress. However, limitations in the topical application of these biomolecules call for novel approaches. This study investigates the efficacy of Pro-GHL, a blend of free fatty acids, acetylglucosamine and GSH amino acid precursors (GAPs), designed to replenish skin lipids, HA and GSH through de novo biosynthesis. Using primary human keratinocytes, Pro-GHL demonstrated superior antioxidant and anti-inflammatory capacities compared to each individual component under the challenge of UVB or blue light. In 3D skin equivalent models (EpiKutis<sup>®</sup>), Pro-GHL enhanced skin barrier function. In addition, Pro-GHL prevented the development of pigmentation in pigmented 3D skin equivalent models (MelaKutis<sup>®</sup>) subjected to UVB irradiation or Benzo[a]pyrene exposure. Together, these results highlight Pro-GHL’s potential as a novel, effective and comprehensive skincare approach to fortify the skin’s defense system from within and prevent the accumulation of tissue damage in response to extrinsic stressors. |
| format | Article |
| id | doaj-art-5ed1125cfdce4116a311a05ab0d626a6 |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-5ed1125cfdce4116a311a05ab0d626a62025-08-20T03:43:26ZengMDPI AGBiology2079-77372025-03-0114326610.3390/biology14030266A Triple-Precursor Blend as a Topical Solution to Protect the Skin Against Environmental DamagePing Gao0Xue Xiao1Zhuang Zhou2Hong Zhang3Raghupathi Subramanian4Anuchai Sinsawat5Xuelan Gu6Unilever R&D Shanghai, 66 Lin Xin Road, Shanghai 202305, ChinaUnilever R&D Shanghai, 66 Lin Xin Road, Shanghai 202305, ChinaUnilever R&D Shanghai, 66 Lin Xin Road, Shanghai 202305, ChinaUnilever R&D Shanghai, 66 Lin Xin Road, Shanghai 202305, ChinaUnilever R&D Trumbull, 55 Merritt Blvd, Trumbull, CT 06611, USAUnilever Thai Holdings Ltd., 411 Srinakarin Road, Suanluang, Bangkok 10250, ThailandUnilever R&D Shanghai, 66 Lin Xin Road, Shanghai 202305, ChinaThe epidermis acts as the body’s primary defense, relying on components like lipids, HA and GSH for skin barrier function, hydration and resistance to oxidative stress. However, limitations in the topical application of these biomolecules call for novel approaches. This study investigates the efficacy of Pro-GHL, a blend of free fatty acids, acetylglucosamine and GSH amino acid precursors (GAPs), designed to replenish skin lipids, HA and GSH through de novo biosynthesis. Using primary human keratinocytes, Pro-GHL demonstrated superior antioxidant and anti-inflammatory capacities compared to each individual component under the challenge of UVB or blue light. In 3D skin equivalent models (EpiKutis<sup>®</sup>), Pro-GHL enhanced skin barrier function. In addition, Pro-GHL prevented the development of pigmentation in pigmented 3D skin equivalent models (MelaKutis<sup>®</sup>) subjected to UVB irradiation or Benzo[a]pyrene exposure. Together, these results highlight Pro-GHL’s potential as a novel, effective and comprehensive skincare approach to fortify the skin’s defense system from within and prevent the accumulation of tissue damage in response to extrinsic stressors.https://www.mdpi.com/2079-7737/14/3/266precursoroxidative stressskin damagebarrier protectionskin living equivalenttranscriptomics |
| spellingShingle | Ping Gao Xue Xiao Zhuang Zhou Hong Zhang Raghupathi Subramanian Anuchai Sinsawat Xuelan Gu A Triple-Precursor Blend as a Topical Solution to Protect the Skin Against Environmental Damage Biology precursor oxidative stress skin damage barrier protection skin living equivalent transcriptomics |
| title | A Triple-Precursor Blend as a Topical Solution to Protect the Skin Against Environmental Damage |
| title_full | A Triple-Precursor Blend as a Topical Solution to Protect the Skin Against Environmental Damage |
| title_fullStr | A Triple-Precursor Blend as a Topical Solution to Protect the Skin Against Environmental Damage |
| title_full_unstemmed | A Triple-Precursor Blend as a Topical Solution to Protect the Skin Against Environmental Damage |
| title_short | A Triple-Precursor Blend as a Topical Solution to Protect the Skin Against Environmental Damage |
| title_sort | triple precursor blend as a topical solution to protect the skin against environmental damage |
| topic | precursor oxidative stress skin damage barrier protection skin living equivalent transcriptomics |
| url | https://www.mdpi.com/2079-7737/14/3/266 |
| work_keys_str_mv | AT pinggao atripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT xuexiao atripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT zhuangzhou atripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT hongzhang atripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT raghupathisubramanian atripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT anuchaisinsawat atripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT xuelangu atripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT pinggao tripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT xuexiao tripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT zhuangzhou tripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT hongzhang tripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT raghupathisubramanian tripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT anuchaisinsawat tripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage AT xuelangu tripleprecursorblendasatopicalsolutiontoprotecttheskinagainstenvironmentaldamage |