A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, Nigeria
As a result of the ever increasing problem of multiresistant bacteria, we instituted a surveillance program with the aim of identifying the basic molecular properties of ESBL in our environment. About 197 isolates of Escherichia coli and Klebsiella pneumoniae were selected and tested for ESBL produc...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Interdisciplinary Perspectives on Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/2014/819896 |
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| author | Folasoge A. Adeyankinnu Babatunde O. Motayo Akinniyi Akinduti John Akinbo Joseph I. Ogiogwa Bukola W. Aboderin R. A. Agunlejika |
| author_facet | Folasoge A. Adeyankinnu Babatunde O. Motayo Akinniyi Akinduti John Akinbo Joseph I. Ogiogwa Bukola W. Aboderin R. A. Agunlejika |
| author_sort | Folasoge A. Adeyankinnu |
| collection | DOAJ |
| description | As a result of the ever increasing problem of multiresistant bacteria, we instituted a surveillance program with the aim of identifying the basic molecular properties of ESBL in our environment. About 197 isolates of Escherichia coli and Klebsiella pneumoniae were selected and tested for ESBL production and antimicrobial susceptibility. Plasmid profiles were determined and curing ability was tested. ESBL prevalence was 26.4% for all isolates tested, with E. coli having a greater proportion. There was absolute resistance to ampicilin, tetracycline, and co-trimaxole among tested isolates. There was above average susceptibility to the 2nd and 3rd generation cephalosporins. Plasmid profiles of tested isolates ranged from 9 kbp to 26 kbp with average of 14.99±2.3 kbp for E. coli and 20.98±1.8 kbp K. pneumoniae, 9.6% of ESBL positive E. coli plasmids were cured, while 3.9% of K. pneumoniae plasmids were cured after treatment. The present study shows an upsurge in ESBL acquisition by gram negative bacteria and evidence of cocirculation of varying subtypes of ESBL with both plasmid transmissible and chromosome encoded subtypes. This calls for universal surveillance and more effort towards molecular epidemiology of this public health treatment. |
| format | Article |
| id | doaj-art-5ed08cd8258d4028af7f1820f9b4e73c |
| institution | Kabale University |
| issn | 1687-708X 1687-7098 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Interdisciplinary Perspectives on Infectious Diseases |
| spelling | doaj-art-5ed08cd8258d4028af7f1820f9b4e73c2025-02-03T01:06:39ZengWileyInterdisciplinary Perspectives on Infectious Diseases1687-708X1687-70982014-01-01201410.1155/2014/819896819896A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, NigeriaFolasoge A. Adeyankinnu0Babatunde O. Motayo1Akinniyi Akinduti2John Akinbo3Joseph I. Ogiogwa4Bukola W. Aboderin5R. A. Agunlejika6Microbiology Unit, Pathology Department, Federal Medical Centre, Abeokuta, NigeriaMicrobiology Unit, Pathology Department, Federal Medical Centre, Abeokuta, NigeriaDepartment of Vet Microbiology and Parasitology, Federal University of Agriculture, Abeokuta, NigeriaMicrobiology Unit, Pathology Department, Federal Medical Centre, Abeokuta, NigeriaMicrobiology Unit, Pathology Department, Federal Medical Centre, Abeokuta, NigeriaMicrobiology Unit, Pathology Department, Federal Medical Centre, Abeokuta, NigeriaDepartment of Medical Microbiology and Parasitology, Obafemi Awolowo University Teaching Hospital, Ile-Ife, NigeriaAs a result of the ever increasing problem of multiresistant bacteria, we instituted a surveillance program with the aim of identifying the basic molecular properties of ESBL in our environment. About 197 isolates of Escherichia coli and Klebsiella pneumoniae were selected and tested for ESBL production and antimicrobial susceptibility. Plasmid profiles were determined and curing ability was tested. ESBL prevalence was 26.4% for all isolates tested, with E. coli having a greater proportion. There was absolute resistance to ampicilin, tetracycline, and co-trimaxole among tested isolates. There was above average susceptibility to the 2nd and 3rd generation cephalosporins. Plasmid profiles of tested isolates ranged from 9 kbp to 26 kbp with average of 14.99±2.3 kbp for E. coli and 20.98±1.8 kbp K. pneumoniae, 9.6% of ESBL positive E. coli plasmids were cured, while 3.9% of K. pneumoniae plasmids were cured after treatment. The present study shows an upsurge in ESBL acquisition by gram negative bacteria and evidence of cocirculation of varying subtypes of ESBL with both plasmid transmissible and chromosome encoded subtypes. This calls for universal surveillance and more effort towards molecular epidemiology of this public health treatment.http://dx.doi.org/10.1155/2014/819896 |
| spellingShingle | Folasoge A. Adeyankinnu Babatunde O. Motayo Akinniyi Akinduti John Akinbo Joseph I. Ogiogwa Bukola W. Aboderin R. A. Agunlejika A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, Nigeria Interdisciplinary Perspectives on Infectious Diseases |
| title | A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, Nigeria |
| title_full | A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, Nigeria |
| title_fullStr | A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, Nigeria |
| title_full_unstemmed | A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, Nigeria |
| title_short | A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, Nigeria |
| title_sort | multicenter study of beta lactamase resistant escherichia coli and klebsiella pneumoniae reveals high level chromosome mediated extended spectrum β lactamase resistance in ogun state nigeria |
| url | http://dx.doi.org/10.1155/2014/819896 |
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