Klebsiella spp. carried by insects as a reservoir of virulence and resistance to antimicrobials
Synanthropic flies contribute to the transport of pathogens between environments, but their role remains underexplored. Previously, our group described 197 bacterial strains obtained from 117 dipterous muscoids collected nearby hospitals in Rio de Janeiro, 35 % showing antimicrobial resistance. Ten...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | One Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352771425001004 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849689435286798336 |
|---|---|
| author | Isabel Carramaschi Leticia Miranda Santos Lery Thiago Pavoni Gomes Chagas Viviane Zahner |
| author_facet | Isabel Carramaschi Leticia Miranda Santos Lery Thiago Pavoni Gomes Chagas Viviane Zahner |
| author_sort | Isabel Carramaschi |
| collection | DOAJ |
| description | Synanthropic flies contribute to the transport of pathogens between environments, but their role remains underexplored. Previously, our group described 197 bacterial strains obtained from 117 dipterous muscoids collected nearby hospitals in Rio de Janeiro, 35 % showing antimicrobial resistance. Ten isolates belonged to Klebsiella genus. Klebsiella pneumoniae is a threat to human health due to high resistance and virulence. We characterized the 10 isolates from the genus Klebsiella isolated from flies, comparing to 4 from patients. Most carried resistance determinants as blaSHV and blaNDM. One of them was MDR. Isolates from flies included ST219 and ST76, clinically relevant. Efflux pumps and porins were sporadically encoded in both fly and clinical samples. Among 10 virulence determinants, fly-isolated strains presented from 2 to 7 genes, while clinical strains ranged from 4 to 6. Notably, fly-isolated formed higher biofilm than clinical. Our findings underline that environmental resistance reservoirs may undermine efforts to control AMR. |
| format | Article |
| id | doaj-art-5ecf17af807544cbbbc269991a1e8c5e |
| institution | DOAJ |
| issn | 2352-7714 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | One Health |
| spelling | doaj-art-5ecf17af807544cbbbc269991a1e8c5e2025-08-20T03:21:39ZengElsevierOne Health2352-77142025-06-012010106410.1016/j.onehlt.2025.101064Klebsiella spp. carried by insects as a reservoir of virulence and resistance to antimicrobialsIsabel Carramaschi0Leticia Miranda Santos Lery1Thiago Pavoni Gomes Chagas2Viviane Zahner3Integrated Laboratory of Simulids and Oncocercosis Medical and Forensics Entomology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil; Cellular Microbiology Laboratory (LAMICEL), Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil; Research and technological development network related to antimicrobial resistance in the one health context - Inova-IOC Program, Rio de Janeiro, BrazilCellular Microbiology Laboratory (LAMICEL), Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil; Research and technological development network related to antimicrobial resistance in the one health context - Inova-IOC Program, Rio de Janeiro, Brazil; Corresponding author at: Av. Brasil, 4365. Pavilhão da Hanseníase, sala 27. Manguinhos, Rio de Janeiro, RJ, Brazil.Molecular Epidemiology and Biotechnology Laboratory (LEMB), Faculty of Pharmacy, Fluminense Federal University, Niterói, Brazil; Department of Pathology, School ofMedicine, Fluminense Federal University, Niterói, BrazilIntegrated Laboratory of Simulids and Oncocercosis Medical and Forensics Entomology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil; Research and technological development network related to antimicrobial resistance in the one health context - Inova-IOC Program, Rio de Janeiro, BrazilSynanthropic flies contribute to the transport of pathogens between environments, but their role remains underexplored. Previously, our group described 197 bacterial strains obtained from 117 dipterous muscoids collected nearby hospitals in Rio de Janeiro, 35 % showing antimicrobial resistance. Ten isolates belonged to Klebsiella genus. Klebsiella pneumoniae is a threat to human health due to high resistance and virulence. We characterized the 10 isolates from the genus Klebsiella isolated from flies, comparing to 4 from patients. Most carried resistance determinants as blaSHV and blaNDM. One of them was MDR. Isolates from flies included ST219 and ST76, clinically relevant. Efflux pumps and porins were sporadically encoded in both fly and clinical samples. Among 10 virulence determinants, fly-isolated strains presented from 2 to 7 genes, while clinical strains ranged from 4 to 6. Notably, fly-isolated formed higher biofilm than clinical. Our findings underline that environmental resistance reservoirs may undermine efforts to control AMR.http://www.sciencedirect.com/science/article/pii/S2352771425001004Antimicrobial resistanceVirulence factorsBlowfliesBacterial infectionOne-healthEnvironmental bacteria |
| spellingShingle | Isabel Carramaschi Leticia Miranda Santos Lery Thiago Pavoni Gomes Chagas Viviane Zahner Klebsiella spp. carried by insects as a reservoir of virulence and resistance to antimicrobials One Health Antimicrobial resistance Virulence factors Blowflies Bacterial infection One-health Environmental bacteria |
| title | Klebsiella spp. carried by insects as a reservoir of virulence and resistance to antimicrobials |
| title_full | Klebsiella spp. carried by insects as a reservoir of virulence and resistance to antimicrobials |
| title_fullStr | Klebsiella spp. carried by insects as a reservoir of virulence and resistance to antimicrobials |
| title_full_unstemmed | Klebsiella spp. carried by insects as a reservoir of virulence and resistance to antimicrobials |
| title_short | Klebsiella spp. carried by insects as a reservoir of virulence and resistance to antimicrobials |
| title_sort | klebsiella spp carried by insects as a reservoir of virulence and resistance to antimicrobials |
| topic | Antimicrobial resistance Virulence factors Blowflies Bacterial infection One-health Environmental bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S2352771425001004 |
| work_keys_str_mv | AT isabelcarramaschi klebsiellasppcarriedbyinsectsasareservoirofvirulenceandresistancetoantimicrobials AT leticiamirandasantoslery klebsiellasppcarriedbyinsectsasareservoirofvirulenceandresistancetoantimicrobials AT thiagopavonigomeschagas klebsiellasppcarriedbyinsectsasareservoirofvirulenceandresistancetoantimicrobials AT vivianezahner klebsiellasppcarriedbyinsectsasareservoirofvirulenceandresistancetoantimicrobials |