Dissemination of a IncHI2A plasmid co-harboring the mcr-9 and bla NDM-1 genes in Israeli hospitals
Abstract The mcr-9 allele is typically located on IncHI2 type plasmids, but there are only few reports describing the co-occurrence of the bla NDM gene on the same plasmid. Our aims were to describe the spread of an IncHI2A plasmid harboring both the mcr-9 and the bla NDM-1 genes in a multicenter st...
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BMC
2025-08-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Online Access: | https://doi.org/10.1186/s12941-025-00814-7 |
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| author | Amos Adler Stefany Ayala-Montaño Mark V. Assous Yuval Geffen Sandra Reuter |
| author_facet | Amos Adler Stefany Ayala-Montaño Mark V. Assous Yuval Geffen Sandra Reuter |
| author_sort | Amos Adler |
| collection | DOAJ |
| description | Abstract The mcr-9 allele is typically located on IncHI2 type plasmids, but there are only few reports describing the co-occurrence of the bla NDM gene on the same plasmid. Our aims were to describe the spread of an IncHI2A plasmid harboring both the mcr-9 and the bla NDM-1 genes in a multicenter study in Israel. All New-Delhi Metallo-β-lactamase-producing Enterobacterales (NDME) isolated from three medical centres in Israel between January 2018 and July 2019 were included. The mcr-9.1 gene was identified in 37/212 (17.4%) of the isolates, mostly in Enterobacter cloacae (34/37, 92%). The mcr-9.1 gene was also identified in Klebsiella pneumoniae sequence types (ST)-76 (n = 2) and Escherichia coli ST-69 (n = 1). In one hospital, out of 32 E. cloacae isolates, 19 (51.35%) were clustered into five transmission clusters of the ST-511, ST-1261 and ST-1750. Four subtypes of a ~ 290 kb IncHI2A type plasmid were identified in all isolates that co-harbored the mcr-9.1 and the bla NDM-1 genes. This plasmid was identified in all isolates, with four sub-communities (sc), with sc-4 identified in all three species. The resistance genes were surrounded by the IS26 (mcr-9.1) or by the ISAba125 and the IS300 (bla NDM-1) mobile elements. The dissemination of the mcr-9.1 and the bla NDM-1 genes was accelerated via clonal spread and the dual carriage on a single plasmid. |
| format | Article |
| id | doaj-art-5ec8003b34d84a55a47dcf662008c456 |
| institution | Kabale University |
| issn | 1476-0711 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj-art-5ec8003b34d84a55a47dcf662008c4562025-08-24T11:12:42ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112025-08-012411410.1186/s12941-025-00814-7Dissemination of a IncHI2A plasmid co-harboring the mcr-9 and bla NDM-1 genes in Israeli hospitalsAmos Adler0Stefany Ayala-Montaño1Mark V. Assous2Yuval Geffen3Sandra Reuter4Clinical Microbiology, Tel Aviv Sourasky Medical CenterInstitute for Infection Prevention and Hospital Epidemiology, Medical Center-University of FreiburgLaboratory of Clinical Microbiology, Faculty of Medicine, Shaare Zedek Medical Center, Hebrew University of JerusalemMicrobiology Laboratory, Rambam Medical CenterInstitute for Infection Prevention and Hospital Epidemiology, Medical Center-University of FreiburgAbstract The mcr-9 allele is typically located on IncHI2 type plasmids, but there are only few reports describing the co-occurrence of the bla NDM gene on the same plasmid. Our aims were to describe the spread of an IncHI2A plasmid harboring both the mcr-9 and the bla NDM-1 genes in a multicenter study in Israel. All New-Delhi Metallo-β-lactamase-producing Enterobacterales (NDME) isolated from three medical centres in Israel between January 2018 and July 2019 were included. The mcr-9.1 gene was identified in 37/212 (17.4%) of the isolates, mostly in Enterobacter cloacae (34/37, 92%). The mcr-9.1 gene was also identified in Klebsiella pneumoniae sequence types (ST)-76 (n = 2) and Escherichia coli ST-69 (n = 1). In one hospital, out of 32 E. cloacae isolates, 19 (51.35%) were clustered into five transmission clusters of the ST-511, ST-1261 and ST-1750. Four subtypes of a ~ 290 kb IncHI2A type plasmid were identified in all isolates that co-harbored the mcr-9.1 and the bla NDM-1 genes. This plasmid was identified in all isolates, with four sub-communities (sc), with sc-4 identified in all three species. The resistance genes were surrounded by the IS26 (mcr-9.1) or by the ISAba125 and the IS300 (bla NDM-1) mobile elements. The dissemination of the mcr-9.1 and the bla NDM-1 genes was accelerated via clonal spread and the dual carriage on a single plasmid.https://doi.org/10.1186/s12941-025-00814-7 |
| spellingShingle | Amos Adler Stefany Ayala-Montaño Mark V. Assous Yuval Geffen Sandra Reuter Dissemination of a IncHI2A plasmid co-harboring the mcr-9 and bla NDM-1 genes in Israeli hospitals Annals of Clinical Microbiology and Antimicrobials |
| title | Dissemination of a IncHI2A plasmid co-harboring the mcr-9 and bla NDM-1 genes in Israeli hospitals |
| title_full | Dissemination of a IncHI2A plasmid co-harboring the mcr-9 and bla NDM-1 genes in Israeli hospitals |
| title_fullStr | Dissemination of a IncHI2A plasmid co-harboring the mcr-9 and bla NDM-1 genes in Israeli hospitals |
| title_full_unstemmed | Dissemination of a IncHI2A plasmid co-harboring the mcr-9 and bla NDM-1 genes in Israeli hospitals |
| title_short | Dissemination of a IncHI2A plasmid co-harboring the mcr-9 and bla NDM-1 genes in Israeli hospitals |
| title_sort | dissemination of a inchi2a plasmid co harboring the mcr 9 and bla ndm 1 genes in israeli hospitals |
| url | https://doi.org/10.1186/s12941-025-00814-7 |
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