Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary Tumors
Inflammation results in the production of cytokines, such as interleukin- (IL-) 4 and IL-10 with immunosuppressive properties or IL-6 and TNF-α with procarcinogenic activity. Furthermore, NF-κB is the major link between inflammation and tumorigenesis. This study verified the interaction between acti...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/9512743 |
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| author | Gustavo Rodrigues Martins Gabriela Bottaro Gelaleti Marina Gobbe Moschetta Larissa Bazela Maschio-Signorini Debora Ap. Pires de Campos Zuccari |
| author_facet | Gustavo Rodrigues Martins Gabriela Bottaro Gelaleti Marina Gobbe Moschetta Larissa Bazela Maschio-Signorini Debora Ap. Pires de Campos Zuccari |
| author_sort | Gustavo Rodrigues Martins |
| collection | DOAJ |
| description | Inflammation results in the production of cytokines, such as interleukin- (IL-) 4 and IL-10 with immunosuppressive properties or IL-6 and TNF-α with procarcinogenic activity. Furthermore, NF-κB is the major link between inflammation and tumorigenesis. This study verified the interaction between active inflammatory cytokines in the tumor microenvironment and serum of female dogs with mammary tumors and their correlation with the clinicopathological characteristics and overall survival. Measurement of gene expression was performed by qPCR and protein levels by ELISA/Luminex. High gene and protein expression levels of NF-κB, IL-6, and TNF-α were found in association with characteristics that reflect worse prognosis and a negative correlation between TNF-α protein expression and survival time was observed (p<0.05). In contrast, high gene and protein expression levels of IL-4 and IL-10 were associated with characteristics of better prognosis and an increased level of IL-4 and a longer survival time of animals were obtained (p<0.05). In addition, there was a positive correlation between TNF-α and IL-6 expression in association with NF-κB. The results show a significant correlation of these cytokines with tumor development, associated with NF-κB expression and cytokines promodulation, showing that these biological factors could be used as predictive and prognostic markers in breast cancer. |
| format | Article |
| id | doaj-art-5ec2d7b630c24c3cba24ec25bdfce8ef |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5ec2d7b630c24c3cba24ec25bdfce8ef2025-08-20T03:34:33ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/95127439512743Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary TumorsGustavo Rodrigues Martins0Gabriela Bottaro Gelaleti1Marina Gobbe Moschetta2Larissa Bazela Maschio-Signorini3Debora Ap. Pires de Campos Zuccari4Laboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Department of Molecular Biology, Graduate Program in Health Science, Avenida Brigadeiro Faria Lima 5416, Vila São Pedro, 15090-000 São José do Rio Preto, SP, BrazilLaboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Department of Biology, Graduate Program in Genetics, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP/IBILCE), Rua Cristóvão Colombo 2265, Jardim Nazareth, 15054-000 São José do Rio Preto, SP, BrazilLaboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Department of Molecular Biology, Graduate Program in Health Science, Avenida Brigadeiro Faria Lima 5416, Vila São Pedro, 15090-000 São José do Rio Preto, SP, BrazilLaboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Department of Biology, Graduate Program in Genetics, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP/IBILCE), Rua Cristóvão Colombo 2265, Jardim Nazareth, 15054-000 São José do Rio Preto, SP, BrazilLaboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Avenida Brigadeiro Faria Lima 5416, Vila São Pedro, 15.090-000 São José do Rio Preto, SP, BrazilInflammation results in the production of cytokines, such as interleukin- (IL-) 4 and IL-10 with immunosuppressive properties or IL-6 and TNF-α with procarcinogenic activity. Furthermore, NF-κB is the major link between inflammation and tumorigenesis. This study verified the interaction between active inflammatory cytokines in the tumor microenvironment and serum of female dogs with mammary tumors and their correlation with the clinicopathological characteristics and overall survival. Measurement of gene expression was performed by qPCR and protein levels by ELISA/Luminex. High gene and protein expression levels of NF-κB, IL-6, and TNF-α were found in association with characteristics that reflect worse prognosis and a negative correlation between TNF-α protein expression and survival time was observed (p<0.05). In contrast, high gene and protein expression levels of IL-4 and IL-10 were associated with characteristics of better prognosis and an increased level of IL-4 and a longer survival time of animals were obtained (p<0.05). In addition, there was a positive correlation between TNF-α and IL-6 expression in association with NF-κB. The results show a significant correlation of these cytokines with tumor development, associated with NF-κB expression and cytokines promodulation, showing that these biological factors could be used as predictive and prognostic markers in breast cancer.http://dx.doi.org/10.1155/2016/9512743 |
| spellingShingle | Gustavo Rodrigues Martins Gabriela Bottaro Gelaleti Marina Gobbe Moschetta Larissa Bazela Maschio-Signorini Debora Ap. Pires de Campos Zuccari Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary Tumors Mediators of Inflammation |
| title | Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary Tumors |
| title_full | Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary Tumors |
| title_fullStr | Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary Tumors |
| title_full_unstemmed | Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary Tumors |
| title_short | Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary Tumors |
| title_sort | proinflammatory and anti inflammatory cytokines mediated by nf κb factor as prognostic markers in mammary tumors |
| url | http://dx.doi.org/10.1155/2016/9512743 |
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