Determination of IFN -γ and TNF -α levels in serum of patients with Cutaneous leishmaniasis
Cutaneous leishmaniasis, is common parasitic disease in Iraq, it is caused by unicellular protozoa that belong to the genus Leishmania which infecting human macrophages, and it is induce human humoral and cellular immune responses. Many cytokines are excreted during this response, however IFN -γ a...
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Tikrit University
2020-12-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/311 |
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| author | Huda M. Taher Shihab A. Mohammed Ayhan R. Mahmood |
| author_facet | Huda M. Taher Shihab A. Mohammed Ayhan R. Mahmood |
| author_sort | Huda M. Taher |
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Cutaneous leishmaniasis, is common parasitic disease in Iraq, it is caused by unicellular protozoa that belong to the genus Leishmania which infecting human macrophages, and it is induce human humoral and cellular immune responses. Many cytokines are excreted during this response, however IFN -γ and TNF -α are the most important factors in the inception of immunity against leishmania spp. The present study aimed to estimate the serum level of TNF-α and IFN-Ƴ in patients with cutaneous leishmaniasis (CL) by enzyme-linked immunosorbent assay (ELISA). Out of 62 patients with CL lesion the infection rate was higher in males 36 (58%) than females 26 (42%), also the highest infection rate 29 (46.8%) was in age group (less than 1 -10years), and the lowest infection rate 18 (2.4%) was in the age group (51-60). Blood samples collected from 62 patients with CL and 27 healthy individual. The results showed that the level of serum IFN-Y (38.23 pg/mL) in patients with CL was significantly higher than control group (21.98 pg/mL). As well as the results indicated a significantly increase in the levels of TNF-α in patients serum with CL (46.53 pg / mL) than control group (23 pg / ml).
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| format | Article |
| id | doaj-art-5ebd6afd37224e09bc285b9408481ae4 |
| institution | OA Journals |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-5ebd6afd37224e09bc285b9408481ae42025-08-20T02:36:45ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262020-12-0125610.25130/tjps.v25i6.311Determination of IFN -γ and TNF -α levels in serum of patients with Cutaneous leishmaniasisHuda M. TaherShihab A. MohammedAyhan R. Mahmood Cutaneous leishmaniasis, is common parasitic disease in Iraq, it is caused by unicellular protozoa that belong to the genus Leishmania which infecting human macrophages, and it is induce human humoral and cellular immune responses. Many cytokines are excreted during this response, however IFN -γ and TNF -α are the most important factors in the inception of immunity against leishmania spp. The present study aimed to estimate the serum level of TNF-α and IFN-Ƴ in patients with cutaneous leishmaniasis (CL) by enzyme-linked immunosorbent assay (ELISA). Out of 62 patients with CL lesion the infection rate was higher in males 36 (58%) than females 26 (42%), also the highest infection rate 29 (46.8%) was in age group (less than 1 -10years), and the lowest infection rate 18 (2.4%) was in the age group (51-60). Blood samples collected from 62 patients with CL and 27 healthy individual. The results showed that the level of serum IFN-Y (38.23 pg/mL) in patients with CL was significantly higher than control group (21.98 pg/mL). As well as the results indicated a significantly increase in the levels of TNF-α in patients serum with CL (46.53 pg / mL) than control group (23 pg / ml). https://tjpsj.org/index.php/tjps/article/view/311Cutaneous leishmaniasisTNF-αIFN-Ƴ |
| spellingShingle | Huda M. Taher Shihab A. Mohammed Ayhan R. Mahmood Determination of IFN -γ and TNF -α levels in serum of patients with Cutaneous leishmaniasis Tikrit Journal of Pure Science Cutaneous leishmaniasis TNF-α IFN-Ƴ |
| title | Determination of IFN -γ and TNF -α levels in serum of patients with Cutaneous leishmaniasis |
| title_full | Determination of IFN -γ and TNF -α levels in serum of patients with Cutaneous leishmaniasis |
| title_fullStr | Determination of IFN -γ and TNF -α levels in serum of patients with Cutaneous leishmaniasis |
| title_full_unstemmed | Determination of IFN -γ and TNF -α levels in serum of patients with Cutaneous leishmaniasis |
| title_short | Determination of IFN -γ and TNF -α levels in serum of patients with Cutaneous leishmaniasis |
| title_sort | determination of ifn γ and tnf α levels in serum of patients with cutaneous leishmaniasis |
| topic | Cutaneous leishmaniasis TNF-α IFN-Ƴ |
| url | https://tjpsj.org/index.php/tjps/article/view/311 |
| work_keys_str_mv | AT hudamtaher determinationofifngandtnfalevelsinserumofpatientswithcutaneousleishmaniasis AT shihabamohammed determinationofifngandtnfalevelsinserumofpatientswithcutaneousleishmaniasis AT ayhanrmahmood determinationofifngandtnfalevelsinserumofpatientswithcutaneousleishmaniasis |