Shift in the metabolic profile of sediment microbial communities during seagrass decline

Abstract Background Seagrass meadows are highly productive ecosystems that are considered hotspots for carbon sequestration and microbial activity. In seagrass sediments, microbial communities break down organic matter, facilitating the release and transformation of nutrients that support plant grow...

Full description

Saved in:
Bibliographic Details
Main Authors: Marsej Markovski, Mirjana Najdek, Zihao Zhao, Gerhard J. Herndl, Marino Korlević
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Environmental Microbiome
Subjects:
Online Access:https://doi.org/10.1186/s40793-025-00750-1
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849341781836038144
author Marsej Markovski
Mirjana Najdek
Zihao Zhao
Gerhard J. Herndl
Marino Korlević
author_facet Marsej Markovski
Mirjana Najdek
Zihao Zhao
Gerhard J. Herndl
Marino Korlević
author_sort Marsej Markovski
collection DOAJ
description Abstract Background Seagrass meadows are highly productive ecosystems that are considered hotspots for carbon sequestration and microbial activity. In seagrass sediments, microbial communities break down organic matter, facilitating the release and transformation of nutrients that support plant growth and primary production. The decline of seagrass meadows of various species has been documented worldwide, including that of Cymodocea nodosa (Ucria) Ascherson, a widespread seagrass in the Mediterranean Sea. To assess the influence of seagrass decline on the metabolic profile of sediment microbial communities, metaproteomes from two sites, one without vegetation and one with a declining Cymodocea nodosa meadow, were characterised at monthly intervals from July 2017 to October 2018. Results Prior to seagrass decline, differences in the metabolic profiles between the vegetated and nonvegetated sediment were found, particularly in the deeper sediment layers. During the decline, these differences diminished as microbial communities in nonvegetated sediments exhibited increased protein richness and diversity, aligning more closely with those at the vegetated site. Notably, temporal variations in the structure of the metabolic profile were only observable in the nonvegetated sediment and were also more pronounced at greater sediment depths. Finally, the assessment of proteins involved in organic matter degradation such as ABC transporters, fermentation-mediating enzymes, and proteins involved in dissimilatory sulphate reduction mirrored these shifts. Conclusions Overall, the main results of this study suggest that the presence of seagrass meadows influences the metabolic profile of microbial communities in sediments, highlighting the distinctions between nonvegetated and seagrass-colonised sediments. In particular, the loss of seagrass leads to a shift in the metabolic profile of sediment communities in the surrounding area, while the metabolic profiles of previously colonised sediments appear to be more resilient to seagrass loss.
format Article
id doaj-art-5eb176a7290b4aeca803f231ffcadf76
institution Kabale University
issn 2524-6372
language English
publishDate 2025-07-01
publisher BMC
record_format Article
series Environmental Microbiome
spelling doaj-art-5eb176a7290b4aeca803f231ffcadf762025-08-20T03:43:34ZengBMCEnvironmental Microbiome2524-63722025-07-0120111810.1186/s40793-025-00750-1Shift in the metabolic profile of sediment microbial communities during seagrass declineMarsej Markovski0Mirjana Najdek1Zihao Zhao2Gerhard J. Herndl3Marino Korlević4Centre for Marine Research, Ruđer Bošković InstituteCentre for Marine Research, Ruđer Bošković InstituteDepartment of Functional and Evolutionary Ecology, University of ViennaDepartment of Functional and Evolutionary Ecology, University of ViennaCentre for Marine Research, Ruđer Bošković InstituteAbstract Background Seagrass meadows are highly productive ecosystems that are considered hotspots for carbon sequestration and microbial activity. In seagrass sediments, microbial communities break down organic matter, facilitating the release and transformation of nutrients that support plant growth and primary production. The decline of seagrass meadows of various species has been documented worldwide, including that of Cymodocea nodosa (Ucria) Ascherson, a widespread seagrass in the Mediterranean Sea. To assess the influence of seagrass decline on the metabolic profile of sediment microbial communities, metaproteomes from two sites, one without vegetation and one with a declining Cymodocea nodosa meadow, were characterised at monthly intervals from July 2017 to October 2018. Results Prior to seagrass decline, differences in the metabolic profiles between the vegetated and nonvegetated sediment were found, particularly in the deeper sediment layers. During the decline, these differences diminished as microbial communities in nonvegetated sediments exhibited increased protein richness and diversity, aligning more closely with those at the vegetated site. Notably, temporal variations in the structure of the metabolic profile were only observable in the nonvegetated sediment and were also more pronounced at greater sediment depths. Finally, the assessment of proteins involved in organic matter degradation such as ABC transporters, fermentation-mediating enzymes, and proteins involved in dissimilatory sulphate reduction mirrored these shifts. Conclusions Overall, the main results of this study suggest that the presence of seagrass meadows influences the metabolic profile of microbial communities in sediments, highlighting the distinctions between nonvegetated and seagrass-colonised sediments. In particular, the loss of seagrass leads to a shift in the metabolic profile of sediment communities in the surrounding area, while the metabolic profiles of previously colonised sediments appear to be more resilient to seagrass loss.https://doi.org/10.1186/s40793-025-00750-1Sediment microbial communitiesCymodocea nodosaSeagrass meadow declineNorthern Adriatic SeaMetaproteomicsMicrobial metabolic profile
spellingShingle Marsej Markovski
Mirjana Najdek
Zihao Zhao
Gerhard J. Herndl
Marino Korlević
Shift in the metabolic profile of sediment microbial communities during seagrass decline
Environmental Microbiome
Sediment microbial communities
Cymodocea nodosa
Seagrass meadow decline
Northern Adriatic Sea
Metaproteomics
Microbial metabolic profile
title Shift in the metabolic profile of sediment microbial communities during seagrass decline
title_full Shift in the metabolic profile of sediment microbial communities during seagrass decline
title_fullStr Shift in the metabolic profile of sediment microbial communities during seagrass decline
title_full_unstemmed Shift in the metabolic profile of sediment microbial communities during seagrass decline
title_short Shift in the metabolic profile of sediment microbial communities during seagrass decline
title_sort shift in the metabolic profile of sediment microbial communities during seagrass decline
topic Sediment microbial communities
Cymodocea nodosa
Seagrass meadow decline
Northern Adriatic Sea
Metaproteomics
Microbial metabolic profile
url https://doi.org/10.1186/s40793-025-00750-1
work_keys_str_mv AT marsejmarkovski shiftinthemetabolicprofileofsedimentmicrobialcommunitiesduringseagrassdecline
AT mirjananajdek shiftinthemetabolicprofileofsedimentmicrobialcommunitiesduringseagrassdecline
AT zihaozhao shiftinthemetabolicprofileofsedimentmicrobialcommunitiesduringseagrassdecline
AT gerhardjherndl shiftinthemetabolicprofileofsedimentmicrobialcommunitiesduringseagrassdecline
AT marinokorlevic shiftinthemetabolicprofileofsedimentmicrobialcommunitiesduringseagrassdecline