Liquid Chromatography-Mass Spectrometry-Based Metabolomic Analysis of Metabolites and Metabolic Pathways of Selenium-Enriched Auricularia auricula
Auricularia auricula was selenized by adding exogenous selenium to the compost. The effect of selenization on the dynamic changes of metabolic pathways and metabolites in A. auricula was investigated using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics combined with different mul...
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China Food Publishing Company
2025-02-01
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-018.pdf |
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| author | YUE Shibo, CUI Hang, SHEN Qin, CAO Dongmei,,, |
| author_facet | YUE Shibo, CUI Hang, SHEN Qin, CAO Dongmei,,, |
| author_sort | YUE Shibo, CUI Hang, SHEN Qin, CAO Dongmei,,, |
| collection | DOAJ |
| description | Auricularia auricula was selenized by adding exogenous selenium to the compost. The effect of selenization on the dynamic changes of metabolic pathways and metabolites in A. auricula was investigated using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics combined with different multivariate statistical analyses such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and hierarchical cluster analysis. Totally 669 metabolites were identified in A. auricula, inorganic selenized A. auricula grown on sodium selenite, and organic selenized A. auricula grown on selenium-rich corn straw. A total of 228 differential metabolites (DEMs) were obtained in A. auricula vs inorganic selenized A. auricula, and 267 DEMs in A. auricula vs organic selenized A. auricula. It was found that selenization had a great effect on the contents of organic acids and their derivatives, lipids and lipid-like molecules in A. auricula, and the effect of organic selenization was more pronounced. Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analysis found that selenization had a significant effect on lipid and amino acid metabolism. These results showed that lipid metabolism, amino acid metabolism and the biosynthesis of other secondary metabolites in A. auricula changed significantly after selenization, which had a significant effect on lipids, amino acids and other metabolites in A. auricula. This indicated that selenization affected the nutritional composition and flavor quality of A. auricula. |
| format | Article |
| id | doaj-art-5ea809bad23341b6bdc170bfb228ad92 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-5ea809bad23341b6bdc170bfb228ad922025-02-05T09:08:33ZengChina Food Publishing CompanyShipin Kexue1002-66302025-02-0146316216810.7506/spkx1002-6630-20240627-198Liquid Chromatography-Mass Spectrometry-Based Metabolomic Analysis of Metabolites and Metabolic Pathways of Selenium-Enriched Auricularia auriculaYUE Shibo, CUI Hang, SHEN Qin, CAO Dongmei,,,0(1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. Mudanjiang Institute of Food and Biotechnology Innovation, Heilongjiang Bayi Agricultural University, Mudanjiang 157000, China; 3. National Coarse Cereals Engineering Research Center, Daqing 163319, China; 4. Weifang Vocational College of Food Science and Technology, Weifang 262123, China; 5. Key Laboratory of Agro-products Processing and Quality Safety of Heilongjiang Province, Daqing 163319, China; 6. Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing 163319, China)Auricularia auricula was selenized by adding exogenous selenium to the compost. The effect of selenization on the dynamic changes of metabolic pathways and metabolites in A. auricula was investigated using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics combined with different multivariate statistical analyses such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and hierarchical cluster analysis. Totally 669 metabolites were identified in A. auricula, inorganic selenized A. auricula grown on sodium selenite, and organic selenized A. auricula grown on selenium-rich corn straw. A total of 228 differential metabolites (DEMs) were obtained in A. auricula vs inorganic selenized A. auricula, and 267 DEMs in A. auricula vs organic selenized A. auricula. It was found that selenization had a great effect on the contents of organic acids and their derivatives, lipids and lipid-like molecules in A. auricula, and the effect of organic selenization was more pronounced. Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analysis found that selenization had a significant effect on lipid and amino acid metabolism. These results showed that lipid metabolism, amino acid metabolism and the biosynthesis of other secondary metabolites in A. auricula changed significantly after selenization, which had a significant effect on lipids, amino acids and other metabolites in A. auricula. This indicated that selenization affected the nutritional composition and flavor quality of A. auricula.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-018.pdfselenization; auricularia auricula; liquid chromatography-mass spectrometry; non-targeted metabolomics |
| spellingShingle | YUE Shibo, CUI Hang, SHEN Qin, CAO Dongmei,,, Liquid Chromatography-Mass Spectrometry-Based Metabolomic Analysis of Metabolites and Metabolic Pathways of Selenium-Enriched Auricularia auricula Shipin Kexue selenization; auricularia auricula; liquid chromatography-mass spectrometry; non-targeted metabolomics |
| title | Liquid Chromatography-Mass Spectrometry-Based Metabolomic Analysis of Metabolites and Metabolic Pathways of Selenium-Enriched Auricularia auricula |
| title_full | Liquid Chromatography-Mass Spectrometry-Based Metabolomic Analysis of Metabolites and Metabolic Pathways of Selenium-Enriched Auricularia auricula |
| title_fullStr | Liquid Chromatography-Mass Spectrometry-Based Metabolomic Analysis of Metabolites and Metabolic Pathways of Selenium-Enriched Auricularia auricula |
| title_full_unstemmed | Liquid Chromatography-Mass Spectrometry-Based Metabolomic Analysis of Metabolites and Metabolic Pathways of Selenium-Enriched Auricularia auricula |
| title_short | Liquid Chromatography-Mass Spectrometry-Based Metabolomic Analysis of Metabolites and Metabolic Pathways of Selenium-Enriched Auricularia auricula |
| title_sort | liquid chromatography mass spectrometry based metabolomic analysis of metabolites and metabolic pathways of selenium enriched auricularia auricula |
| topic | selenization; auricularia auricula; liquid chromatography-mass spectrometry; non-targeted metabolomics |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-018.pdf |
| work_keys_str_mv | AT yueshibocuihangshenqincaodongmei liquidchromatographymassspectrometrybasedmetabolomicanalysisofmetabolitesandmetabolicpathwaysofseleniumenrichedauriculariaauricula |