Identification and Purification of Cholera Like Toxin from Environmental Isolate of Vibrio cholerae
The presence and prevalence of V. cholerae were investigated in forty five water samples collected from different locations of Tiger River/ Baghdad city. Twenty one isolates were isolated by adopting a simple isolation techniques. The final identification revealed that only three isolates were confi...
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| Format: | Article |
| Language: | English |
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University of Baghdad, College of Science for Women
2010-03-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2865 |
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| author | Baghdad Science Journal |
| author_facet | Baghdad Science Journal |
| author_sort | Baghdad Science Journal |
| collection | DOAJ |
| description | The presence and prevalence of V. cholerae were investigated in forty five water samples collected from different locations of Tiger River/ Baghdad city. Twenty one isolates were isolated by adopting a simple isolation techniques. The final identification revealed that only three isolates were confirmed as V. cholerae. They were named 1J, 1R and Dial 131 which are all serogrouped as non-O1.
Toxin Coregulated Pili (TCP) and heat labile enterotoxin (LT) were determined in only the environmental isolate 1J while non of the isolates produced heat stabile toxin (ST).
The purification scheme was improved, few steps were adopted to include back extraction of ammonium sulfate, saturation between 80-20%, desalting through Sephadex G25, and gel filtration using Sephadex G100 which highly increase the specific activity. |
| format | Article |
| id | doaj-art-5ea6ada024e04a94a26c1b30137b4583 |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2010-03-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-5ea6ada024e04a94a26c1b30137b45832025-08-20T03:56:17ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862010-03-017110.21123/bsj.7.1.415-423Identification and Purification of Cholera Like Toxin from Environmental Isolate of Vibrio choleraeBaghdad Science JournalThe presence and prevalence of V. cholerae were investigated in forty five water samples collected from different locations of Tiger River/ Baghdad city. Twenty one isolates were isolated by adopting a simple isolation techniques. The final identification revealed that only three isolates were confirmed as V. cholerae. They were named 1J, 1R and Dial 131 which are all serogrouped as non-O1. Toxin Coregulated Pili (TCP) and heat labile enterotoxin (LT) were determined in only the environmental isolate 1J while non of the isolates produced heat stabile toxin (ST). The purification scheme was improved, few steps were adopted to include back extraction of ammonium sulfate, saturation between 80-20%, desalting through Sephadex G25, and gel filtration using Sephadex G100 which highly increase the specific activity.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2865"environmental Vibrio cholerae, cholera like toxin, radial immune diffusion" |
| spellingShingle | Baghdad Science Journal Identification and Purification of Cholera Like Toxin from Environmental Isolate of Vibrio cholerae مجلة بغداد للعلوم "environmental Vibrio cholerae, cholera like toxin, radial immune diffusion" |
| title | Identification and Purification of Cholera Like Toxin from Environmental Isolate of Vibrio cholerae |
| title_full | Identification and Purification of Cholera Like Toxin from Environmental Isolate of Vibrio cholerae |
| title_fullStr | Identification and Purification of Cholera Like Toxin from Environmental Isolate of Vibrio cholerae |
| title_full_unstemmed | Identification and Purification of Cholera Like Toxin from Environmental Isolate of Vibrio cholerae |
| title_short | Identification and Purification of Cholera Like Toxin from Environmental Isolate of Vibrio cholerae |
| title_sort | identification and purification of cholera like toxin from environmental isolate of vibrio cholerae |
| topic | "environmental Vibrio cholerae, cholera like toxin, radial immune diffusion" |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2865 |
| work_keys_str_mv | AT baghdadsciencejournal identificationandpurificationofcholeraliketoxinfromenvironmentalisolateofvibriocholerae |