Identification of Differentially Expressed Genes from Ovarian Cancer Cells by MICROMAX™ cDNA Microarray System

Using the MICROMAX™ cDNA microarray system, we were able to identify genes that are differentially overexpressed in ovarian cancer. A total of 30 putative genes, which are differentially overexpressed in ovarian cancer cell lines, were identified. The differential expression of some of these genes w...

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Main Authors: Kwong-Kwok Wong, Rita S. Cheng, Samuel C. Mok
Format: Article
Language:English
Published: Taylor & Francis Group 2001-03-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/01303dd05
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author Kwong-Kwok Wong
Rita S. Cheng
Samuel C. Mok
author_facet Kwong-Kwok Wong
Rita S. Cheng
Samuel C. Mok
author_sort Kwong-Kwok Wong
collection DOAJ
description Using the MICROMAX™ cDNA microarray system, we were able to identify genes that are differentially overexpressed in ovarian cancer. A total of 30 putative genes, which are differentially overexpressed in ovarian cancer cell lines, were identified. The differential expression of some of these genes was further confirmed by real-time RT-PCR. Using this strategy, we have identified genes that either overexpress in all cancer cell lines or in only some cancer cell lines. Further characterization of these genes will allow them to be exploited in diagnosis, prognosis, anticancer therapy, and molecular classification of ovarian cancer.
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1940-9818
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spelling doaj-art-5ea5d5dafb334678bc2d7fd03ecbb7052025-08-20T02:25:51ZengTaylor & Francis GroupBioTechniques0736-62051940-98182001-03-0130367067510.2144/01303dd05Identification of Differentially Expressed Genes from Ovarian Cancer Cells by MICROMAX™ cDNA Microarray SystemKwong-Kwok Wong0Rita S. Cheng1Samuel C. Mok21Pacific Northwest National Laboratory, Molecular Biosciences, Richland, WA, USA1Pacific Northwest National Laboratory, Molecular Biosciences, Richland, WA, USA1Pacific Northwest National Laboratory, Molecular Biosciences, Richland, WA, USAUsing the MICROMAX™ cDNA microarray system, we were able to identify genes that are differentially overexpressed in ovarian cancer. A total of 30 putative genes, which are differentially overexpressed in ovarian cancer cell lines, were identified. The differential expression of some of these genes was further confirmed by real-time RT-PCR. Using this strategy, we have identified genes that either overexpress in all cancer cell lines or in only some cancer cell lines. Further characterization of these genes will allow them to be exploited in diagnosis, prognosis, anticancer therapy, and molecular classification of ovarian cancer.https://www.future-science.com/doi/10.2144/01303dd05
spellingShingle Kwong-Kwok Wong
Rita S. Cheng
Samuel C. Mok
Identification of Differentially Expressed Genes from Ovarian Cancer Cells by MICROMAX™ cDNA Microarray System
BioTechniques
title Identification of Differentially Expressed Genes from Ovarian Cancer Cells by MICROMAX™ cDNA Microarray System
title_full Identification of Differentially Expressed Genes from Ovarian Cancer Cells by MICROMAX™ cDNA Microarray System
title_fullStr Identification of Differentially Expressed Genes from Ovarian Cancer Cells by MICROMAX™ cDNA Microarray System
title_full_unstemmed Identification of Differentially Expressed Genes from Ovarian Cancer Cells by MICROMAX™ cDNA Microarray System
title_short Identification of Differentially Expressed Genes from Ovarian Cancer Cells by MICROMAX™ cDNA Microarray System
title_sort identification of differentially expressed genes from ovarian cancer cells by micromax™ cdna microarray system
url https://www.future-science.com/doi/10.2144/01303dd05
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AT samuelcmok identificationofdifferentiallyexpressedgenesfromovariancancercellsbymicromaxcdnamicroarraysystem