Identification and Characterization of Endophytic Fungus DJE2023 Isolated from Banana (<i>Musa</i> sp. cv. Dajiao) with Potential for Biocontrol of Banana Fusarium Wilt
This study characterized an endophytic fungus, DJE2023, isolated from healthy banana sucker of the cultivar (cv.) Dajiao. Its potential as a biocontrol agent against banana Fusarium wilt was assessed, aiming to provide a novel candidate strain for the biological control of the devastating disease. T...
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2024-12-01
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| author | Longqi Jin Rong Huang Jia Zhang Zifeng Li Ruicheng Li Yunfeng Li Guanghui Kong Pinggen Xi Zide Jiang Minhui Li |
| author_facet | Longqi Jin Rong Huang Jia Zhang Zifeng Li Ruicheng Li Yunfeng Li Guanghui Kong Pinggen Xi Zide Jiang Minhui Li |
| author_sort | Longqi Jin |
| collection | DOAJ |
| description | This study characterized an endophytic fungus, DJE2023, isolated from healthy banana sucker of the cultivar (cv.) Dajiao. Its potential as a biocontrol agent against banana Fusarium wilt was assessed, aiming to provide a novel candidate strain for the biological control of the devastating disease. The fungus was isolated using standard plant tissue separation techniques and fungal culture methods, followed by identification through morphological comparisons, multi-gene phylogenetic analyses, and molecular detection targeting <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (<i>Foc</i>) race 1 and race 4. Furthermore, assessments of its characteristics and antagonistic effects were conducted through pathogenicity tests, biological trait investigations, and dual-culture experiments. The results confirmed isolate DJE2023 to be a member of the <i>Fusarium oxysporum</i> species complex but distinct from <i>Foc</i> race 1 or race 4, exhibiting no pathogenicity to banana plantlets of cv. Fenza No.1 or tomato seedlings cv. money maker. Only minute and brown necrotic spots were observed at the rhizomes of banana plantlets of ‘Dajiao’ and ‘Baxijiao’ upon inoculation, contrasting markedly with the extensive necrosis induced by <i>Foc</i> tropical race 4 strain XJZ2 at those of banana cv Baxijiao. Notably, co-inoculation with DJE2023 and XJZ2 revealed a significantly reduced disease severity compared to inoculation with XJZ2 alone. An in vitro plate confrontation assay showed no significant antagonistic effects against <i>Foc</i>, indicating a suppressive effect rather than direct antagonism of DJE2023. Research on the biological characteristics of DJE2023 indicated lactose as the optimal carbon source for its growth, while maltose favored sporulation. The optimal growth temperature for this strain is 28 °C, and its spores can germinate effectively within the range of 25–45 °C and pH 4–10, demonstrating a strong alkali tolerance. Collectively, our findings suggest that DJE2023 exhibits weak or non-pathogenic properties and lacks direct antagonism against <i>Foc</i>, yet imparts a degree of resistance against banana Fusarium wilt. The detailed information provides valuable insight into the potential role of DJE2023 in integrated banana disease control, presenting a promising candidate for biocontrol against banana Fusarium wilt. |
| format | Article |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-5ea2bc1a3e554ae2b14fda2259de1fbb2025-08-20T02:56:48ZengMDPI AGJournal of Fungi2309-608X2024-12-01101287710.3390/jof10120877Identification and Characterization of Endophytic Fungus DJE2023 Isolated from Banana (<i>Musa</i> sp. cv. Dajiao) with Potential for Biocontrol of Banana Fusarium WiltLongqi Jin0Rong Huang1Jia Zhang2Zifeng Li3Ruicheng Li4Yunfeng Li5Guanghui Kong6Pinggen Xi7Zide Jiang8Minhui Li9Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaThis study characterized an endophytic fungus, DJE2023, isolated from healthy banana sucker of the cultivar (cv.) Dajiao. Its potential as a biocontrol agent against banana Fusarium wilt was assessed, aiming to provide a novel candidate strain for the biological control of the devastating disease. The fungus was isolated using standard plant tissue separation techniques and fungal culture methods, followed by identification through morphological comparisons, multi-gene phylogenetic analyses, and molecular detection targeting <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (<i>Foc</i>) race 1 and race 4. Furthermore, assessments of its characteristics and antagonistic effects were conducted through pathogenicity tests, biological trait investigations, and dual-culture experiments. The results confirmed isolate DJE2023 to be a member of the <i>Fusarium oxysporum</i> species complex but distinct from <i>Foc</i> race 1 or race 4, exhibiting no pathogenicity to banana plantlets of cv. Fenza No.1 or tomato seedlings cv. money maker. Only minute and brown necrotic spots were observed at the rhizomes of banana plantlets of ‘Dajiao’ and ‘Baxijiao’ upon inoculation, contrasting markedly with the extensive necrosis induced by <i>Foc</i> tropical race 4 strain XJZ2 at those of banana cv Baxijiao. Notably, co-inoculation with DJE2023 and XJZ2 revealed a significantly reduced disease severity compared to inoculation with XJZ2 alone. An in vitro plate confrontation assay showed no significant antagonistic effects against <i>Foc</i>, indicating a suppressive effect rather than direct antagonism of DJE2023. Research on the biological characteristics of DJE2023 indicated lactose as the optimal carbon source for its growth, while maltose favored sporulation. The optimal growth temperature for this strain is 28 °C, and its spores can germinate effectively within the range of 25–45 °C and pH 4–10, demonstrating a strong alkali tolerance. Collectively, our findings suggest that DJE2023 exhibits weak or non-pathogenic properties and lacks direct antagonism against <i>Foc</i>, yet imparts a degree of resistance against banana Fusarium wilt. The detailed information provides valuable insight into the potential role of DJE2023 in integrated banana disease control, presenting a promising candidate for biocontrol against banana Fusarium wilt.https://www.mdpi.com/2309-608X/10/12/877banana cv. Dajiaoendophytic fungipathogenicityantagonistic activitybiological controlbanana Fusarium wilt |
| spellingShingle | Longqi Jin Rong Huang Jia Zhang Zifeng Li Ruicheng Li Yunfeng Li Guanghui Kong Pinggen Xi Zide Jiang Minhui Li Identification and Characterization of Endophytic Fungus DJE2023 Isolated from Banana (<i>Musa</i> sp. cv. Dajiao) with Potential for Biocontrol of Banana Fusarium Wilt Journal of Fungi banana cv. Dajiao endophytic fungi pathogenicity antagonistic activity biological control banana Fusarium wilt |
| title | Identification and Characterization of Endophytic Fungus DJE2023 Isolated from Banana (<i>Musa</i> sp. cv. Dajiao) with Potential for Biocontrol of Banana Fusarium Wilt |
| title_full | Identification and Characterization of Endophytic Fungus DJE2023 Isolated from Banana (<i>Musa</i> sp. cv. Dajiao) with Potential for Biocontrol of Banana Fusarium Wilt |
| title_fullStr | Identification and Characterization of Endophytic Fungus DJE2023 Isolated from Banana (<i>Musa</i> sp. cv. Dajiao) with Potential for Biocontrol of Banana Fusarium Wilt |
| title_full_unstemmed | Identification and Characterization of Endophytic Fungus DJE2023 Isolated from Banana (<i>Musa</i> sp. cv. Dajiao) with Potential for Biocontrol of Banana Fusarium Wilt |
| title_short | Identification and Characterization of Endophytic Fungus DJE2023 Isolated from Banana (<i>Musa</i> sp. cv. Dajiao) with Potential for Biocontrol of Banana Fusarium Wilt |
| title_sort | identification and characterization of endophytic fungus dje2023 isolated from banana i musa i sp cv dajiao with potential for biocontrol of banana fusarium wilt |
| topic | banana cv. Dajiao endophytic fungi pathogenicity antagonistic activity biological control banana Fusarium wilt |
| url | https://www.mdpi.com/2309-608X/10/12/877 |
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