Isolating Astrocyte-Derived Extracellular Vesicles From Urine
Xin-hui Xie,1,* Mian-mian Chen,1,* Shu-xian Xu,1 Junhua Mei,1,2 Qing Yang,2 Chao Wang,1 Honggang Lyu,1 Qian Gong,1 Zhongchun Liu1,3 1Department of Psychiatry, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China; 2Department of Neurology, Wuhan First Hospital...
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Dove Medical Press
2025-02-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/isolating-astrocyte-derived-extracellular-vesicles-from-urine-peer-reviewed-fulltext-article-IJN |
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| author | Xie XH Chen MM Xu SX Mei J Yang Q Wang C Lyu H Gong Q Liu Z |
| author_facet | Xie XH Chen MM Xu SX Mei J Yang Q Wang C Lyu H Gong Q Liu Z |
| author_sort | Xie XH |
| collection | DOAJ |
| description | Xin-hui Xie,1,* Mian-mian Chen,1,* Shu-xian Xu,1 Junhua Mei,1,2 Qing Yang,2 Chao Wang,1 Honggang Lyu,1 Qian Gong,1 Zhongchun Liu1,3 1Department of Psychiatry, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China; 2Department of Neurology, Wuhan First Hospital, Wuhan, Hubei, People’s Republic of China; 3Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xin-hui Xie; Zhongchun Liu, Department of Psychiatry, Renmin Hospital of Wuhan University, No. 99 Jiefang Road, Wuchang District, Wuhan, Hubei, 430060, People’s Republic of China, Email xxh.med@gmail.com; xin-hui.xie@whu.edu.cn; zcliu6@whu.edu.cn.Introduction: Brain-derived extracellular vesicles (BDEVs) can cross the blood-brain barrier and enter the periphery. Therefore, quantifying and analyzing peripherally circulating BDEVs offer a promising approach to directly obtain a window into central nervous system (CNS) pathobiology in vivo. Rapidly evolving CNS diseases require high-frequency sampling, but daily venipuncture of human subjects is highly invasive and usually unfeasible.Methods: To address this challenge, here we present a novel method for isolating astrocyte-derived extracellular vesicles from urine (uADEVs), combining urine concentration, ultracentrifugation to isolate total EVs, and then glutamate-aspartate transporter (GLAST) EV isolation using an anti-GLAST antibody.Results: The identity of these GLAST+EVs as uADEVs was confirmed by transmission electron microscopy, nanoparticle tracking analysis, western blotting, and assessment of astrocyte-related neurotrophins.Conclusions: Leveraging the convenience and availability of urine samples, the non-invasive uADEV approach provides a novel tool that allows high-frequency sampling to investigate rapidly evolving CNS diseases.Keywords: urinary astrocyte-derived extracellular vesicles, human in vivo, non-invasive, central nervous system, high-frequency sampling |
| format | Article |
| id | doaj-art-5e8589e3fdfa4da5b246cddafcd7dde6 |
| institution | DOAJ |
| issn | 1178-2013 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj-art-5e8589e3fdfa4da5b246cddafcd7dde62025-08-20T02:45:11ZengDove Medical PressInternational Journal of Nanomedicine1178-20132025-02-01Volume 2024752484100574Isolating Astrocyte-Derived Extracellular Vesicles From UrineXie XHChen MMXu SXMei JYang QWang CLyu HGong QLiu ZXin-hui Xie,1,* Mian-mian Chen,1,* Shu-xian Xu,1 Junhua Mei,1,2 Qing Yang,2 Chao Wang,1 Honggang Lyu,1 Qian Gong,1 Zhongchun Liu1,3 1Department of Psychiatry, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China; 2Department of Neurology, Wuhan First Hospital, Wuhan, Hubei, People’s Republic of China; 3Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xin-hui Xie; Zhongchun Liu, Department of Psychiatry, Renmin Hospital of Wuhan University, No. 99 Jiefang Road, Wuchang District, Wuhan, Hubei, 430060, People’s Republic of China, Email xxh.med@gmail.com; xin-hui.xie@whu.edu.cn; zcliu6@whu.edu.cn.Introduction: Brain-derived extracellular vesicles (BDEVs) can cross the blood-brain barrier and enter the periphery. Therefore, quantifying and analyzing peripherally circulating BDEVs offer a promising approach to directly obtain a window into central nervous system (CNS) pathobiology in vivo. Rapidly evolving CNS diseases require high-frequency sampling, but daily venipuncture of human subjects is highly invasive and usually unfeasible.Methods: To address this challenge, here we present a novel method for isolating astrocyte-derived extracellular vesicles from urine (uADEVs), combining urine concentration, ultracentrifugation to isolate total EVs, and then glutamate-aspartate transporter (GLAST) EV isolation using an anti-GLAST antibody.Results: The identity of these GLAST+EVs as uADEVs was confirmed by transmission electron microscopy, nanoparticle tracking analysis, western blotting, and assessment of astrocyte-related neurotrophins.Conclusions: Leveraging the convenience and availability of urine samples, the non-invasive uADEV approach provides a novel tool that allows high-frequency sampling to investigate rapidly evolving CNS diseases.Keywords: urinary astrocyte-derived extracellular vesicles, human in vivo, non-invasive, central nervous system, high-frequency samplinghttps://www.dovepress.com/isolating-astrocyte-derived-extracellular-vesicles-from-urine-peer-reviewed-fulltext-article-IJNurinary astrocyte-derived extracellular vesicleshuman in vivonon-invasivecentral nervous systemhigh-frequency sampling |
| spellingShingle | Xie XH Chen MM Xu SX Mei J Yang Q Wang C Lyu H Gong Q Liu Z Isolating Astrocyte-Derived Extracellular Vesicles From Urine International Journal of Nanomedicine urinary astrocyte-derived extracellular vesicles human in vivo non-invasive central nervous system high-frequency sampling |
| title | Isolating Astrocyte-Derived Extracellular Vesicles From Urine |
| title_full | Isolating Astrocyte-Derived Extracellular Vesicles From Urine |
| title_fullStr | Isolating Astrocyte-Derived Extracellular Vesicles From Urine |
| title_full_unstemmed | Isolating Astrocyte-Derived Extracellular Vesicles From Urine |
| title_short | Isolating Astrocyte-Derived Extracellular Vesicles From Urine |
| title_sort | isolating astrocyte derived extracellular vesicles from urine |
| topic | urinary astrocyte-derived extracellular vesicles human in vivo non-invasive central nervous system high-frequency sampling |
| url | https://www.dovepress.com/isolating-astrocyte-derived-extracellular-vesicles-from-urine-peer-reviewed-fulltext-article-IJN |
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