Isolating Astrocyte-Derived Extracellular Vesicles From Urine
Xin-hui Xie,1,* Mian-mian Chen,1,* Shu-xian Xu,1 Junhua Mei,1,2 Qing Yang,2 Chao Wang,1 Honggang Lyu,1 Qian Gong,1 Zhongchun Liu1,3 1Department of Psychiatry, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China; 2Department of Neurology, Wuhan First Hospital...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-02-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/isolating-astrocyte-derived-extracellular-vesicles-from-urine-peer-reviewed-fulltext-article-IJN |
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| Summary: | Xin-hui Xie,1,* Mian-mian Chen,1,* Shu-xian Xu,1 Junhua Mei,1,2 Qing Yang,2 Chao Wang,1 Honggang Lyu,1 Qian Gong,1 Zhongchun Liu1,3 1Department of Psychiatry, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China; 2Department of Neurology, Wuhan First Hospital, Wuhan, Hubei, People’s Republic of China; 3Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xin-hui Xie; Zhongchun Liu, Department of Psychiatry, Renmin Hospital of Wuhan University, No. 99 Jiefang Road, Wuchang District, Wuhan, Hubei, 430060, People’s Republic of China, Email xxh.med@gmail.com; xin-hui.xie@whu.edu.cn; zcliu6@whu.edu.cn.Introduction: Brain-derived extracellular vesicles (BDEVs) can cross the blood-brain barrier and enter the periphery. Therefore, quantifying and analyzing peripherally circulating BDEVs offer a promising approach to directly obtain a window into central nervous system (CNS) pathobiology in vivo. Rapidly evolving CNS diseases require high-frequency sampling, but daily venipuncture of human subjects is highly invasive and usually unfeasible.Methods: To address this challenge, here we present a novel method for isolating astrocyte-derived extracellular vesicles from urine (uADEVs), combining urine concentration, ultracentrifugation to isolate total EVs, and then glutamate-aspartate transporter (GLAST) EV isolation using an anti-GLAST antibody.Results: The identity of these GLAST+EVs as uADEVs was confirmed by transmission electron microscopy, nanoparticle tracking analysis, western blotting, and assessment of astrocyte-related neurotrophins.Conclusions: Leveraging the convenience and availability of urine samples, the non-invasive uADEV approach provides a novel tool that allows high-frequency sampling to investigate rapidly evolving CNS diseases.Keywords: urinary astrocyte-derived extracellular vesicles, human in vivo, non-invasive, central nervous system, high-frequency sampling |
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| ISSN: | 1178-2013 |