Similarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itch
Dorsal root ganglion (DRG) neurons are responsible for the primary detection and transmission of peripheral noxious stimuli, mainly pain and itch. However, as two distinct noxious sensations, how DRG neurons respond differently to and code pain and itch is still an attractive topic. Here, we investi...
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2025-02-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2024202 |
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| author | Liu Li Yin Jiemin Meng Youqiang Ye Congrui Chen Junhui Wang Sa Yin Wen Gao Po Jiao Yingfu Yu Weifeng Fan Yinghui |
| author_facet | Liu Li Yin Jiemin Meng Youqiang Ye Congrui Chen Junhui Wang Sa Yin Wen Gao Po Jiao Yingfu Yu Weifeng Fan Yinghui |
| author_sort | Liu Li |
| collection | DOAJ |
| description | Dorsal root ganglion (DRG) neurons are responsible for the primary detection and transmission of peripheral noxious stimuli, mainly pain and itch. However, as two distinct noxious sensations, how DRG neurons respond differently to and code pain and itch is still an attractive topic. Here, we investigate the response and activation spectrum of DRG neurons under peripheral pain and itch stimuli using in vivo two-photon calcium imaging and find differences in the response intensity to pain and itch between multisensory neurons (both pain and itch) and single-sensory neurons (either pain or itch). In addition, single-cell RNA sequencing (scRNA-seq) is used to reveal the heterogeneity of distinct subpopulations on the basis of their expressions of pain- or itch-related marker genes and to determine the similarities and differences in their transcriptomic changes under chronic pain and itch. Our results show that primary sensory neurons with different sensory patterns respond differently to the same nociceptive stimuli. Additionally, distinct clusters of neurons exhibit unique transcriptomic changes in the development of chronic pain and itch, which may offer new insights for treating these conditions. |
| format | Article |
| id | doaj-art-5e807f130ed64cc5ae08955b76fb6472 |
| institution | Kabale University |
| issn | 1672-9145 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-5e807f130ed64cc5ae08955b76fb64722025-08-20T03:29:18ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452025-02-015789090010.3724/abbs.202420220d259ccSimilarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itchLiu Li0Yin Jiemin1Meng Youqiang2Ye Congrui3Chen Junhui4Wang Sa5Yin Wen6Gao Po7Jiao Yingfu8Yu Weifeng9Fan Yinghui10["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]["Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China","Department of Neurosurgery, Chongming Hospital Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai 200127, China"]["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]["Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China","Key Laboratory of Anesthesiology (Shanghai Jiao Tong University), Ministry of Education, Shanghai 200127, China"]Dorsal root ganglion (DRG) neurons are responsible for the primary detection and transmission of peripheral noxious stimuli, mainly pain and itch. However, as two distinct noxious sensations, how DRG neurons respond differently to and code pain and itch is still an attractive topic. Here, we investigate the response and activation spectrum of DRG neurons under peripheral pain and itch stimuli using in vivo two-photon calcium imaging and find differences in the response intensity to pain and itch between multisensory neurons (both pain and itch) and single-sensory neurons (either pain or itch). In addition, single-cell RNA sequencing (scRNA-seq) is used to reveal the heterogeneity of distinct subpopulations on the basis of their expressions of pain- or itch-related marker genes and to determine the similarities and differences in their transcriptomic changes under chronic pain and itch. Our results show that primary sensory neurons with different sensory patterns respond differently to the same nociceptive stimuli. Additionally, distinct clusters of neurons exhibit unique transcriptomic changes in the development of chronic pain and itch, which may offer new insights for treating these conditions.https://www.sciengine.com/doi/10.3724/abbs.2024202<italic>in vivo</italic> two-photon calcium imagingdorsal root ganglion (DRG)pain and itch stimulisingle-cell RNA sequencing |
| spellingShingle | Liu Li Yin Jiemin Meng Youqiang Ye Congrui Chen Junhui Wang Sa Yin Wen Gao Po Jiao Yingfu Yu Weifeng Fan Yinghui Similarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itch Acta Biochimica et Biophysica Sinica <italic>in vivo</italic> two-photon calcium imaging dorsal root ganglion (DRG) pain and itch stimuli single-cell RNA sequencing |
| title | Similarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itch |
| title_full | Similarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itch |
| title_fullStr | Similarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itch |
| title_full_unstemmed | Similarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itch |
| title_short | Similarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itch |
| title_sort | similarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itch |
| topic | <italic>in vivo</italic> two-photon calcium imaging dorsal root ganglion (DRG) pain and itch stimuli single-cell RNA sequencing |
| url | https://www.sciengine.com/doi/10.3724/abbs.2024202 |
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