GENETIC VARIATIONS IN THE MAPK 15 GENE AND THEIR RELATIONSHIP WITH MILK PRODUCTION CHARACTERISTICS IN MURRAH BUFFALO
The current research explored the link between genetic diversity in the Mitogen-activated protein kinase 15 (MAPK 15) gene of buffalo and the traits related to milk composition and production, employing PCR-SSCP and Sanger sequencing techniques. The MAPK 15 gene, situated on bovine chromosome 14...
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| Format: | Article |
| Language: | English |
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West Bengal Veterinary Alumni Association
2024-12-01
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| Series: | Exploratory Animal and Medical Research |
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| Online Access: | https://animalmedicalresearch.org/Vol.14_Issue-2_December_2024/GENETIC%20VARIATIONS%20IN%20THE%20MAPK15%20GENE%20AND%20THEIR%20RELATIONSHIP.pdf |
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| author | Sapna Nath Dayal Nitai Das Satish Kumar Illa Mundhe UT Divya P Maneesh Kumar A Satish Chandra |
| author_facet | Sapna Nath Dayal Nitai Das Satish Kumar Illa Mundhe UT Divya P Maneesh Kumar A Satish Chandra |
| author_sort | Sapna Nath |
| collection | DOAJ |
| description | The current research explored the link between genetic diversity in the Mitogen-activated
protein kinase 15 (MAPK 15) gene of buffalo and the traits related to milk composition and production,
employing PCR-SSCP and Sanger sequencing techniques. The MAPK 15 gene, situated on bovine
chromosome 14 and encompassing 1,892 base pairs, consists of 14 exons and encodes the enzyme Mitogenactivated protein kinase 15 (566 amino acids). This enzyme plays an essential role in the development of
the mammary glands and the lactation process. It engages in intracellular signalling pathways that oversee
various cellular functions, including growth and differentiation, suggesting its potential impact on milk
production and the properties of milk composition. The modified high-salt approach was used to extract
genomic DNA from blood samples taken from 100 Murrah buffaloes. PCR amplified products were
resolved by 8% non-denaturing PAGE. Resolved PCR products were genotyped according to the band
pattern during SSCP. The examination uncovered a uniform pattern during PCR-SSCP, hinting at a likely
lack of variation, which implies a significant level of preservation in Murrah buffaloes. The uniform
structure of the MAPK15 gene in this research can assist in distinguishing the Murrah breed from various
other buffalo breeds. The candidate gene needs further validation in a larger cohort for use in the selection
of buffalo cows with required milk composition traits. |
| format | Article |
| id | doaj-art-5e5f762a95e445dbb0c6bcf87307d8f4 |
| institution | DOAJ |
| issn | 2277-470X 2319-247X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | West Bengal Veterinary Alumni Association |
| record_format | Article |
| series | Exploratory Animal and Medical Research |
| spelling | doaj-art-5e5f762a95e445dbb0c6bcf87307d8f42025-08-20T02:58:25ZengWest Bengal Veterinary Alumni AssociationExploratory Animal and Medical Research2277-470X2319-247X2024-12-0114231632410.52635/eamr/14.2.316-324GENETIC VARIATIONS IN THE MAPK 15 GENE AND THEIR RELATIONSHIP WITH MILK PRODUCTION CHARACTERISTICS IN MURRAH BUFFALOSapna Nath0Dayal Nitai Das1Satish Kumar Illa2 Mundhe UT 3Divya P 4Maneesh Kumar A5Satish Chandra6Dairy Production Section, ICAR-NDRI, Karnal, India.ICAR-NDRI (SRS), Bengaluru, India.Dairy Production Section, ICAR-NDRI, Karnal, India.ICAR-NDRI (SRS), Bengaluru, India.ICAR-NDRI (SRS), Bengaluru, India.ICAR-NDRI (SRS), Bengaluru, India.Biological E. Limited, Azamabad, Hyderabad, India.The current research explored the link between genetic diversity in the Mitogen-activated protein kinase 15 (MAPK 15) gene of buffalo and the traits related to milk composition and production, employing PCR-SSCP and Sanger sequencing techniques. The MAPK 15 gene, situated on bovine chromosome 14 and encompassing 1,892 base pairs, consists of 14 exons and encodes the enzyme Mitogenactivated protein kinase 15 (566 amino acids). This enzyme plays an essential role in the development of the mammary glands and the lactation process. It engages in intracellular signalling pathways that oversee various cellular functions, including growth and differentiation, suggesting its potential impact on milk production and the properties of milk composition. The modified high-salt approach was used to extract genomic DNA from blood samples taken from 100 Murrah buffaloes. PCR amplified products were resolved by 8% non-denaturing PAGE. Resolved PCR products were genotyped according to the band pattern during SSCP. The examination uncovered a uniform pattern during PCR-SSCP, hinting at a likely lack of variation, which implies a significant level of preservation in Murrah buffaloes. The uniform structure of the MAPK15 gene in this research can assist in distinguishing the Murrah breed from various other buffalo breeds. The candidate gene needs further validation in a larger cohort for use in the selection of buffalo cows with required milk composition traits.https://animalmedicalresearch.org/Vol.14_Issue-2_December_2024/GENETIC%20VARIATIONS%20IN%20THE%20MAPK15%20GENE%20AND%20THEIR%20RELATIONSHIP.pdfmapk 15 genepcr-sscpexpressionassociationmilk composition traitsmurrah buffolo |
| spellingShingle | Sapna Nath Dayal Nitai Das Satish Kumar Illa Mundhe UT Divya P Maneesh Kumar A Satish Chandra GENETIC VARIATIONS IN THE MAPK 15 GENE AND THEIR RELATIONSHIP WITH MILK PRODUCTION CHARACTERISTICS IN MURRAH BUFFALO Exploratory Animal and Medical Research mapk 15 gene pcr-sscp expression association milk composition traits murrah buffolo |
| title | GENETIC VARIATIONS IN THE MAPK 15 GENE AND THEIR RELATIONSHIP WITH MILK PRODUCTION CHARACTERISTICS IN MURRAH BUFFALO |
| title_full | GENETIC VARIATIONS IN THE MAPK 15 GENE AND THEIR RELATIONSHIP WITH MILK PRODUCTION CHARACTERISTICS IN MURRAH BUFFALO |
| title_fullStr | GENETIC VARIATIONS IN THE MAPK 15 GENE AND THEIR RELATIONSHIP WITH MILK PRODUCTION CHARACTERISTICS IN MURRAH BUFFALO |
| title_full_unstemmed | GENETIC VARIATIONS IN THE MAPK 15 GENE AND THEIR RELATIONSHIP WITH MILK PRODUCTION CHARACTERISTICS IN MURRAH BUFFALO |
| title_short | GENETIC VARIATIONS IN THE MAPK 15 GENE AND THEIR RELATIONSHIP WITH MILK PRODUCTION CHARACTERISTICS IN MURRAH BUFFALO |
| title_sort | genetic variations in the mapk 15 gene and their relationship with milk production characteristics in murrah buffalo |
| topic | mapk 15 gene pcr-sscp expression association milk composition traits murrah buffolo |
| url | https://animalmedicalresearch.org/Vol.14_Issue-2_December_2024/GENETIC%20VARIATIONS%20IN%20THE%20MAPK15%20GENE%20AND%20THEIR%20RELATIONSHIP.pdf |
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