AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINE
trichothecenes and their derivatives have recently attracted much attention of researchers with respect of their potential role in medicine, including for the treatment of different types of cancer. The purpose of the study was to investigate the cytotoxic effect of Fusarium trichothecene on human b...
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| Format: | Article |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2020-01-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/1248 |
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| author | Rin. S. Mukhammadiev Rish. S. Mukhammadiev V. V. Biryulya I. I. Idiyatov A. A. Nabatov A. P. Glinushkin L. R. Valiullin |
| author_facet | Rin. S. Mukhammadiev Rish. S. Mukhammadiev V. V. Biryulya I. I. Idiyatov A. A. Nabatov A. P. Glinushkin L. R. Valiullin |
| author_sort | Rin. S. Mukhammadiev |
| collection | DOAJ |
| description | trichothecenes and their derivatives have recently attracted much attention of researchers with respect of their potential role in medicine, including for the treatment of different types of cancer. The purpose of the study was to investigate the cytotoxic effect of Fusarium trichothecene on human breast cancer cells, human skin fibroblasts and embryonic kidney cells in vitro. Material and methods. Based on the Mtt assay, the cytotoxic effect of trichothecene on cell cultures was determined. Evaluation of morphological changes in cell cultures under the influence of trichothecene was performed by light microscopy. Results. Fusarium trichothecene was found to exhibit a dose-dependent toxic effect on cell lines in the range 1 nM to 1000 nM. the most pronounced cytotoxic effect of trichothecene was observed in human breast cancer cell line (IС50 94.72 ± 4.1 нМ). Lower doses of trichothecene led to a change in the size, shape of human breast cancer cells, human skin fibroblasts and embryonic kidney cells, and loss of contact between them and their isolation. the degradation of cell membranes, formation of unformed cell aggregates and fragments were observed in higher doses of trichothecene. Conclusion. Fusarium trichothecen is a biologically active compound and is less toxic to the normal than to the cancer cell lines, therefore, further studies of this agent are needed. |
| format | Article |
| id | doaj-art-5e5d894697c64244b72eea8d5e011146 |
| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2020-01-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-5e5d894697c64244b72eea8d5e0111462025-08-20T03:35:48ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682020-01-01186909510.21294/1814-4861-2019-18-6-90-95694AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINERin. S. Mukhammadiev0Rish. S. Mukhammadiev1V. V. Biryulya2I. I. Idiyatov3A. A. Nabatov4A. P. Glinushkin5L. R. Valiullin6Federal center of Toxicological, Radiological and Biological SafetyFederal center of Toxicological, Radiological and Biological SafetyFederal center of Toxicological, Radiological and Biological SafetyFederal center of Toxicological, Radiological and Biological SafetyKazan State Medical AcademyAll-Russia Research Institute of PhytopatologyFederal center of Toxicological, Radiological and Biological Safetytrichothecenes and their derivatives have recently attracted much attention of researchers with respect of their potential role in medicine, including for the treatment of different types of cancer. The purpose of the study was to investigate the cytotoxic effect of Fusarium trichothecene on human breast cancer cells, human skin fibroblasts and embryonic kidney cells in vitro. Material and methods. Based on the Mtt assay, the cytotoxic effect of trichothecene on cell cultures was determined. Evaluation of morphological changes in cell cultures under the influence of trichothecene was performed by light microscopy. Results. Fusarium trichothecene was found to exhibit a dose-dependent toxic effect on cell lines in the range 1 nM to 1000 nM. the most pronounced cytotoxic effect of trichothecene was observed in human breast cancer cell line (IС50 94.72 ± 4.1 нМ). Lower doses of trichothecene led to a change in the size, shape of human breast cancer cells, human skin fibroblasts and embryonic kidney cells, and loss of contact between them and their isolation. the degradation of cell membranes, formation of unformed cell aggregates and fragments were observed in higher doses of trichothecene. Conclusion. Fusarium trichothecen is a biologically active compound and is less toxic to the normal than to the cancer cell lines, therefore, further studies of this agent are needed.https://www.siboncoj.ru/jour/article/view/1248trichotheceneculturebreast cancerskin fibroblastsembryo kidneyscytotoxicity |
| spellingShingle | Rin. S. Mukhammadiev Rish. S. Mukhammadiev V. V. Biryulya I. I. Idiyatov A. A. Nabatov A. P. Glinushkin L. R. Valiullin AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINE Сибирский онкологический журнал trichothecene culture breast cancer skin fibroblasts embryo kidneys cytotoxicity |
| title | AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINE |
| title_full | AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINE |
| title_fullStr | AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINE |
| title_full_unstemmed | AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINE |
| title_short | AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINE |
| title_sort | in vitro estimation of cytotoxicity of fusarium trichothecene on the breast cancer cell line |
| topic | trichothecene culture breast cancer skin fibroblasts embryo kidneys cytotoxicity |
| url | https://www.siboncoj.ru/jour/article/view/1248 |
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