The Role of microRNA in Overactive Bladder: Relationship and Clinical Correlation
<i>Background and Objectives</i>: This study aimed to determine the relationship between miRNAs and overactive bladder (OAB). We also aimed to reveal the diagnostic properties of miRNAs and their potential to predict responses to therapy. <i>Materials and Methods:</i> The stu...
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MDPI AG
2025-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/61/3/475 |
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| author | Kürşat Küçüker Hülya Aybek Hakan Akça Ege Rıza Karagür Elif Fırat Yusuf Özlülerden Sinan Çelen Zafer Aybek |
| author_facet | Kürşat Küçüker Hülya Aybek Hakan Akça Ege Rıza Karagür Elif Fırat Yusuf Özlülerden Sinan Çelen Zafer Aybek |
| author_sort | Kürşat Küçüker |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: This study aimed to determine the relationship between miRNAs and overactive bladder (OAB). We also aimed to reveal the diagnostic properties of miRNAs and their potential to predict responses to therapy. <i>Materials and Methods:</i> The study included 60 patients with OAB as the treatment group and 60 healthy individuals as the control group. The blood levels of 15 miRNAs in both groups were determined using PCR. Also, miRNAs with high diagnostic values were identified with receiver operating characteristic (ROC) curves. Finally, the Turkish-validated OAB questionnaire form was filled out before and after the treatment by the participants in the treatment group. In this way, the relationship between OAB score changes and miRNA levels was examined. <i>Results:</i> The let-7a, let-7c, let-7e, let-7f, and let-7g miRNA molecules in the treatment group were higher, with a high level of significance (<i>p</i> = 0.0001). Additionally, the miR-135b, miR-300, miR-372, miR-373, miR-381, miR-520a, miR-520d, and miR-520e miRNA molecules were found to be statistically higher in the control group (<i>p</i> = 0.0001). In addition, let-7c (area under curve [AUC] = 0.985) and the let-7c + miR-381 combination (area under curve [AUC] = 1) were the highest values in the ROC analysis. Finally, after treatment in the patient group, a significant difference was detected in both miRNAs (let-7f and miR-135b) in patients with clinical improvements of 50% and above in the OAB score. <i>Conclusions:</i> miRNAs may help elucidate the pathophysiology of OAB. They may shed light on diagnosis and evaluation of treatment effectiveness. |
| format | Article |
| id | doaj-art-5e52fa2f0b984bcc9c0f58c6296e641b |
| institution | DOAJ |
| issn | 1010-660X 1648-9144 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj-art-5e52fa2f0b984bcc9c0f58c6296e641b2025-08-20T02:42:22ZengMDPI AGMedicina1010-660X1648-91442025-03-0161347510.3390/medicina61030475The Role of microRNA in Overactive Bladder: Relationship and Clinical CorrelationKürşat Küçüker0Hülya Aybek1Hakan Akça2Ege Rıza Karagür3Elif Fırat4Yusuf Özlülerden5Sinan Çelen6Zafer Aybek7Department of Urology, Pamukkale University School of Medicine, Denizli 20070, TurkeyDepartment of Medical Biochemistry, Pamukkale University School of Medicine, Denizli 20070, TurkeyDepartment of Medical Genetics, Pamukkale University School of Medicine, Denizli 20070, TurkeyDepartment of Medical Genetics, Pamukkale University School of Medicine, Denizli 20070, TurkeyDepartment of Medical Biochemistry, Manisa Celal Bayar University School of Medicine, Manisa 45140, TurkeyDepartment of Urology, Pamukkale University School of Medicine, Denizli 20070, TurkeyDepartment of Urology, Pamukkale University School of Medicine, Denizli 20070, TurkeyDepartment of Urology, Pamukkale University School of Medicine, Denizli 20070, Turkey<i>Background and Objectives</i>: This study aimed to determine the relationship between miRNAs and overactive bladder (OAB). We also aimed to reveal the diagnostic properties of miRNAs and their potential to predict responses to therapy. <i>Materials and Methods:</i> The study included 60 patients with OAB as the treatment group and 60 healthy individuals as the control group. The blood levels of 15 miRNAs in both groups were determined using PCR. Also, miRNAs with high diagnostic values were identified with receiver operating characteristic (ROC) curves. Finally, the Turkish-validated OAB questionnaire form was filled out before and after the treatment by the participants in the treatment group. In this way, the relationship between OAB score changes and miRNA levels was examined. <i>Results:</i> The let-7a, let-7c, let-7e, let-7f, and let-7g miRNA molecules in the treatment group were higher, with a high level of significance (<i>p</i> = 0.0001). Additionally, the miR-135b, miR-300, miR-372, miR-373, miR-381, miR-520a, miR-520d, and miR-520e miRNA molecules were found to be statistically higher in the control group (<i>p</i> = 0.0001). In addition, let-7c (area under curve [AUC] = 0.985) and the let-7c + miR-381 combination (area under curve [AUC] = 1) were the highest values in the ROC analysis. Finally, after treatment in the patient group, a significant difference was detected in both miRNAs (let-7f and miR-135b) in patients with clinical improvements of 50% and above in the OAB score. <i>Conclusions:</i> miRNAs may help elucidate the pathophysiology of OAB. They may shed light on diagnosis and evaluation of treatment effectiveness.https://www.mdpi.com/1648-9144/61/3/475overactive bladdermicroRNAbiomarkercholinergic |
| spellingShingle | Kürşat Küçüker Hülya Aybek Hakan Akça Ege Rıza Karagür Elif Fırat Yusuf Özlülerden Sinan Çelen Zafer Aybek The Role of microRNA in Overactive Bladder: Relationship and Clinical Correlation Medicina overactive bladder microRNA biomarker cholinergic |
| title | The Role of microRNA in Overactive Bladder: Relationship and Clinical Correlation |
| title_full | The Role of microRNA in Overactive Bladder: Relationship and Clinical Correlation |
| title_fullStr | The Role of microRNA in Overactive Bladder: Relationship and Clinical Correlation |
| title_full_unstemmed | The Role of microRNA in Overactive Bladder: Relationship and Clinical Correlation |
| title_short | The Role of microRNA in Overactive Bladder: Relationship and Clinical Correlation |
| title_sort | role of microrna in overactive bladder relationship and clinical correlation |
| topic | overactive bladder microRNA biomarker cholinergic |
| url | https://www.mdpi.com/1648-9144/61/3/475 |
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