Performance, physiological and immune responses of Nile tilapia Oreochromis niloticus fed extruded pellet diets with different binders

The trial was conducted to investigate the effects of inclusion carboxymethyl cellulose (CMC) or calcium lignosulfonate (CLS) on the physical qualities of extruded Nile tilapia diet, growth performance, feed efficiency, physiological and immune response for 70-day. Three identical isonitrogenous and...

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Main Authors: Eman Y. Mohammady, Ümit Acar, Elsayed M. Younis, Abdel-Wahab A. Abdel-Warith, Simon J. Davies, Ehab R. El-Haroun, Mohamed S. Hassaan
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352513425003308
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author Eman Y. Mohammady
Ümit Acar
Elsayed M. Younis
Abdel-Wahab A. Abdel-Warith
Simon J. Davies
Ehab R. El-Haroun
Mohamed S. Hassaan
author_facet Eman Y. Mohammady
Ümit Acar
Elsayed M. Younis
Abdel-Wahab A. Abdel-Warith
Simon J. Davies
Ehab R. El-Haroun
Mohamed S. Hassaan
author_sort Eman Y. Mohammady
collection DOAJ
description The trial was conducted to investigate the effects of inclusion carboxymethyl cellulose (CMC) or calcium lignosulfonate (CLS) on the physical qualities of extruded Nile tilapia diet, growth performance, feed efficiency, physiological and immune response for 70-day. Three identical isonitrogenous and isoenergetic diet were formulated, 0 (control), 4 g CMC and 4 g CLS kg−1 diet and fed to tilapia with an average initial body weight (14.20 ± 1.22 g). Inclusion of CLS significantly improved the water stability, durability, bulk density, and sinking speed of the pellets compared to CMC and control diets (P ≤ 0.05). Compared to the control group, inclusion of 4 g kg−1 CLS recorded the highest weight gain (WG, 51.00 g fish−1), specific growth rate (SGR, 3.02 %, day fish−1) and aveage daily gain (ADG) (0.61 g fish−1). The highest activities of amylase (84.102.44 U L−1), lipase (958.3 ± 70.11 L) and trypsin (0.59 ± 0.18 ng ml−1) were detected in fish fed CLS group. The height and width of villi and goblet cell number in both the anterior and posterior intestines were significantly increased in fish fed CLS than other groups. The activities of alanine amino transferase (ALT) and aspertate aminotransferase (AST) levels substantially decreased in the CLS group compared to the control. While CLS supplementation significantly elevated serum total protein, globulin, and albumin levels compared with CMC group and control diet. No significant differences were found in serum lipid profile among fish fed experimental diets. Catalase and superoxide dismutase (SOD) were significanly higher in fish fed diet supplemented with 4 g CLS kg−1 compared with others group. Furthermore, immunoglobulin M (IgM) and complement factors (C3, C4), were significantly improved in fish fed diet contained CLS.
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publishDate 2025-09-01
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series Aquaculture Reports
spelling doaj-art-5e44c09c9d2646d69ee6d0ad67ac2ef92025-08-20T03:45:12ZengElsevierAquaculture Reports2352-51342025-09-014310294410.1016/j.aqrep.2025.102944Performance, physiological and immune responses of Nile tilapia Oreochromis niloticus fed extruded pellet diets with different bindersEman Y. Mohammady0Ümit Acar1Elsayed M. Younis2Abdel-Wahab A. Abdel-Warith3Simon J. Davies4Ehab R. El-Haroun5Mohamed S. Hassaan6Aquaculture Division, National Institute of Oceanography and Fisheries, NIOF, Cairo, EgyptÇanakkale Onsekiz Mart University, Bayramiç Vocational School, Department of Forestry, Çanakkale, TurkeyDepartment of Zoology, College of science, King Saudi University, Riyadh, Saudi ArabiaDepartment of Zoology, College of science, King Saudi University, Riyadh, Saudi ArabiaCarna Research Station. Ryan institute, aquaculture nutrition research unit ANRU, College of science and Engineering, University of Galway, IrelandDepartment of Integrative Agriculture, College of Agriculture and Veterinary Medicine, United Arab Emirates University, P.O. Box 15551, Abu Dhabi, Al Ain, United Arab Emirates.; Corresponding author.Department of Animal Production, Fish Research Laboratory, Faculty of Agriculture at Moshtohor, Benha, University, Benha 13736, EgyptThe trial was conducted to investigate the effects of inclusion carboxymethyl cellulose (CMC) or calcium lignosulfonate (CLS) on the physical qualities of extruded Nile tilapia diet, growth performance, feed efficiency, physiological and immune response for 70-day. Three identical isonitrogenous and isoenergetic diet were formulated, 0 (control), 4 g CMC and 4 g CLS kg−1 diet and fed to tilapia with an average initial body weight (14.20 ± 1.22 g). Inclusion of CLS significantly improved the water stability, durability, bulk density, and sinking speed of the pellets compared to CMC and control diets (P ≤ 0.05). Compared to the control group, inclusion of 4 g kg−1 CLS recorded the highest weight gain (WG, 51.00 g fish−1), specific growth rate (SGR, 3.02 %, day fish−1) and aveage daily gain (ADG) (0.61 g fish−1). The highest activities of amylase (84.102.44 U L−1), lipase (958.3 ± 70.11 L) and trypsin (0.59 ± 0.18 ng ml−1) were detected in fish fed CLS group. The height and width of villi and goblet cell number in both the anterior and posterior intestines were significantly increased in fish fed CLS than other groups. The activities of alanine amino transferase (ALT) and aspertate aminotransferase (AST) levels substantially decreased in the CLS group compared to the control. While CLS supplementation significantly elevated serum total protein, globulin, and albumin levels compared with CMC group and control diet. No significant differences were found in serum lipid profile among fish fed experimental diets. Catalase and superoxide dismutase (SOD) were significanly higher in fish fed diet supplemented with 4 g CLS kg−1 compared with others group. Furthermore, immunoglobulin M (IgM) and complement factors (C3, C4), were significantly improved in fish fed diet contained CLS.http://www.sciencedirect.com/science/article/pii/S2352513425003308GrowthNile tilapiaPellets binderSerum biochemicalsImmunty
spellingShingle Eman Y. Mohammady
Ümit Acar
Elsayed M. Younis
Abdel-Wahab A. Abdel-Warith
Simon J. Davies
Ehab R. El-Haroun
Mohamed S. Hassaan
Performance, physiological and immune responses of Nile tilapia Oreochromis niloticus fed extruded pellet diets with different binders
Aquaculture Reports
Growth
Nile tilapia
Pellets binder
Serum biochemicals
Immunty
title Performance, physiological and immune responses of Nile tilapia Oreochromis niloticus fed extruded pellet diets with different binders
title_full Performance, physiological and immune responses of Nile tilapia Oreochromis niloticus fed extruded pellet diets with different binders
title_fullStr Performance, physiological and immune responses of Nile tilapia Oreochromis niloticus fed extruded pellet diets with different binders
title_full_unstemmed Performance, physiological and immune responses of Nile tilapia Oreochromis niloticus fed extruded pellet diets with different binders
title_short Performance, physiological and immune responses of Nile tilapia Oreochromis niloticus fed extruded pellet diets with different binders
title_sort performance physiological and immune responses of nile tilapia oreochromis niloticus fed extruded pellet diets with different binders
topic Growth
Nile tilapia
Pellets binder
Serum biochemicals
Immunty
url http://www.sciencedirect.com/science/article/pii/S2352513425003308
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