Investigating antimicrobial resistance genes in probiotic products for companion animals
IntroductionOne of the greatest challenges of our time is antimicrobial resistance, which could become the leading cause of death globally within a few decades. In the context of One Health, it is in the common interest to mitigate the global spread of antimicrobial resistance by seeking alternative...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-10-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1464351/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850114806572384256 |
|---|---|
| author | Adam Kerek Adam Kerek Emese Szabó Ábel Szabó Márton Papp Márton Papp Krisztián Bányai Krisztián Bányai Krisztián Bányai Gábor Kardos Gábor Kardos Gábor Kardos Gábor Kardos Eszter Kaszab Eszter Kaszab Eszter Kaszab Krisztina Bali Krisztina Bali Ákos Jerzsele Ákos Jerzsele |
| author_facet | Adam Kerek Adam Kerek Emese Szabó Ábel Szabó Márton Papp Márton Papp Krisztián Bányai Krisztián Bányai Krisztián Bányai Gábor Kardos Gábor Kardos Gábor Kardos Gábor Kardos Eszter Kaszab Eszter Kaszab Eszter Kaszab Krisztina Bali Krisztina Bali Ákos Jerzsele Ákos Jerzsele |
| author_sort | Adam Kerek |
| collection | DOAJ |
| description | IntroductionOne of the greatest challenges of our time is antimicrobial resistance, which could become the leading cause of death globally within a few decades. In the context of One Health, it is in the common interest to mitigate the global spread of antimicrobial resistance by seeking alternative solutions, alongside appropriate drug selection and responsible use. Probiotics offer a potential avenue to reduce antibiotic usage; however, there is a scarcity of research that examines commercial products in terms of carrying antimicrobial resistance genes (ARGs) involved in resistance development through microbial vectors.MethodsOur study investigated 10 commercially available probiotic products for cats and dogs. Initially, we conducted phenotypic testing through determination of minimum inhibitory concentration (MIC) for antibiotics important in animal and public health. Subsequently, we performed next-generation sequencing (NGS) of the products to elucidate the genetic background behind the decrease in phenotypic sensitivity.ResultsIn total, 19 types of ARGs were identified, with 57.9% being found on plasmids, and in two cases, carriage as mobile genetic elements were found. One of the genes identified was the APH(3′)-Ia gene, capable of inactivating aminoglycoside antibiotics through phosphotransferase enzyme production regulation, while the other was the tetS gene, capable of conferring reduced sensitivity to tetracycline antibiotics through target protection.DiscussionOur findings underscore the importance of approaching antimicrobial resistance investigations from a broader perspective. We suggest that further studies in this area are justified and raise questions regarding the need to extend legally required studies on probiotic products from their use in economic livestock to their use in companion animals. |
| format | Article |
| id | doaj-art-5e3d2b7e7dec486e86c35a9c39156a64 |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-5e3d2b7e7dec486e86c35a9c39156a642025-08-20T02:36:45ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-10-011110.3389/fvets.2024.14643511464351Investigating antimicrobial resistance genes in probiotic products for companion animalsAdam Kerek0Adam Kerek1Emese Szabó2Ábel Szabó3Márton Papp4Márton Papp5Krisztián Bányai6Krisztián Bányai7Krisztián Bányai8Gábor Kardos9Gábor Kardos10Gábor Kardos11Gábor Kardos12Eszter Kaszab13Eszter Kaszab14Eszter Kaszab15Krisztina Bali16Krisztina Bali17Ákos Jerzsele18Ákos Jerzsele19Department of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, HungaryCentre for Bioinformatics, University of Veterinary Medicine, Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, HungaryVeterinary Medical Research Institute, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, HungaryOne Health Institute, Faculty of Health Sciences, University of Debrecen, Debrecen, HungaryNational Public Health Center, Budapest, HungaryDepartment of Metagenomics, University of Debrecen, Debrecen, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, HungaryOne Health Institute, Faculty of Health Sciences, University of Debrecen, Debrecen, HungaryDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, HungaryDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, HungaryIntroductionOne of the greatest challenges of our time is antimicrobial resistance, which could become the leading cause of death globally within a few decades. In the context of One Health, it is in the common interest to mitigate the global spread of antimicrobial resistance by seeking alternative solutions, alongside appropriate drug selection and responsible use. Probiotics offer a potential avenue to reduce antibiotic usage; however, there is a scarcity of research that examines commercial products in terms of carrying antimicrobial resistance genes (ARGs) involved in resistance development through microbial vectors.MethodsOur study investigated 10 commercially available probiotic products for cats and dogs. Initially, we conducted phenotypic testing through determination of minimum inhibitory concentration (MIC) for antibiotics important in animal and public health. Subsequently, we performed next-generation sequencing (NGS) of the products to elucidate the genetic background behind the decrease in phenotypic sensitivity.ResultsIn total, 19 types of ARGs were identified, with 57.9% being found on plasmids, and in two cases, carriage as mobile genetic elements were found. One of the genes identified was the APH(3′)-Ia gene, capable of inactivating aminoglycoside antibiotics through phosphotransferase enzyme production regulation, while the other was the tetS gene, capable of conferring reduced sensitivity to tetracycline antibiotics through target protection.DiscussionOur findings underscore the importance of approaching antimicrobial resistance investigations from a broader perspective. We suggest that further studies in this area are justified and raise questions regarding the need to extend legally required studies on probiotic products from their use in economic livestock to their use in companion animals.https://www.frontiersin.org/articles/10.3389/fvets.2024.1464351/fullprobioticsARGNGScompanion animalsantimicrobial resistance |
| spellingShingle | Adam Kerek Adam Kerek Emese Szabó Ábel Szabó Márton Papp Márton Papp Krisztián Bányai Krisztián Bányai Krisztián Bányai Gábor Kardos Gábor Kardos Gábor Kardos Gábor Kardos Eszter Kaszab Eszter Kaszab Eszter Kaszab Krisztina Bali Krisztina Bali Ákos Jerzsele Ákos Jerzsele Investigating antimicrobial resistance genes in probiotic products for companion animals Frontiers in Veterinary Science probiotics ARG NGS companion animals antimicrobial resistance |
| title | Investigating antimicrobial resistance genes in probiotic products for companion animals |
| title_full | Investigating antimicrobial resistance genes in probiotic products for companion animals |
| title_fullStr | Investigating antimicrobial resistance genes in probiotic products for companion animals |
| title_full_unstemmed | Investigating antimicrobial resistance genes in probiotic products for companion animals |
| title_short | Investigating antimicrobial resistance genes in probiotic products for companion animals |
| title_sort | investigating antimicrobial resistance genes in probiotic products for companion animals |
| topic | probiotics ARG NGS companion animals antimicrobial resistance |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1464351/full |
| work_keys_str_mv | AT adamkerek investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT adamkerek investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT emeseszabo investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT abelszabo investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT martonpapp investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT martonpapp investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT krisztianbanyai investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT krisztianbanyai investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT krisztianbanyai investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT gaborkardos investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT gaborkardos investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT gaborkardos investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT gaborkardos investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT eszterkaszab investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT eszterkaszab investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT eszterkaszab investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT krisztinabali investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT krisztinabali investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT akosjerzsele investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals AT akosjerzsele investigatingantimicrobialresistancegenesinprobioticproductsforcompanionanimals |