Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis
Abstract Objective Vaginal lactobacilli play a crucial role in inhibiting bacteria such as Gardnerella vaginalis (G. vaginalis), a key contributor to dysbiosis and bacterial vaginosis. We aimed to compare the inhibitory effects of Lactobacillus cell-free supernatants on G. vaginalis growth with thos...
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BMC
2025-08-01
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| Online Access: | https://doi.org/10.1186/s13104-025-07414-w |
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| author | Ferenc Somogyvári Nándor Tűzkő Attila Kereszturi László Párducz Mária Szécsényi Valéria Endrész Marianna Ábrók Caleb M. Ardizzone Katalin Burián Dezső Péter Virok |
| author_facet | Ferenc Somogyvári Nándor Tűzkő Attila Kereszturi László Párducz Mária Szécsényi Valéria Endrész Marianna Ábrók Caleb M. Ardizzone Katalin Burián Dezső Péter Virok |
| author_sort | Ferenc Somogyvári |
| collection | DOAJ |
| description | Abstract Objective Vaginal lactobacilli play a crucial role in inhibiting bacteria such as Gardnerella vaginalis (G. vaginalis), a key contributor to dysbiosis and bacterial vaginosis. We aimed to compare the inhibitory effects of Lactobacillus cell-free supernatants on G. vaginalis growth with those observed in Lactobacillus-G. vaginalis coculture, an experimental setup that more closely mimics in vivo conditions. Results To identify the optimal medium for coculture experiments, MRS broth and NYC-III broth were compared. NYC-III significantly enhanced the growth of G. vaginalis and four of the five tested Lactobacillus strains. We then developed a direct quantitative PCR (qPCR) method that allowed us to specifically measure G. vaginalis genome concentrations in cocultures with Lactobacillus. This direct qPCR did not require DNA extraction and had a 4,096-fold dynamic range. We then assessed the inhibition of G. vaginalis growth by Lactobacillus cell-free supernatants in G. vaginalis cultures by measuring optical density and the Lactobacillus-mediated inhibition in cocultures by the G. vaginalis specific direct qPCR. The two measurement methods showed different levels of inhibitory activity for two of the five Lactobacillus strains tested. These findings suggest that coculture experiments should be conducted in place of, or in addition to, supernatant-based inhibitory assays. |
| format | Article |
| id | doaj-art-5e3be45bdef7487fb443b0cd25b6a211 |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-5e3be45bdef7487fb443b0cd25b6a2112025-08-20T03:42:19ZengBMCBMC Research Notes1756-05002025-08-011811710.1186/s13104-025-07414-wComparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalisFerenc Somogyvári0Nándor Tűzkő1Attila Kereszturi2László Párducz3Mária Szécsényi4Valéria Endrész5Marianna Ábrók6Caleb M. Ardizzone7Katalin Burián8Dezső Péter Virok9Department of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of SzegedDepartment of Obstetrics and Gynecology, St. Margaret’s HospitalDepartment of Obstetrics and Gynecology, Albert Szent-Györgyi Health Center, Faculty of Medicine, University of SzegedDepartment of Obstetrics and Gynecology, Pándy Kálmán County HospitalDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of SzegedDivision of Infectious Diseases, Department of Medicine, Indiana University School of MedicineDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of SzegedAbstract Objective Vaginal lactobacilli play a crucial role in inhibiting bacteria such as Gardnerella vaginalis (G. vaginalis), a key contributor to dysbiosis and bacterial vaginosis. We aimed to compare the inhibitory effects of Lactobacillus cell-free supernatants on G. vaginalis growth with those observed in Lactobacillus-G. vaginalis coculture, an experimental setup that more closely mimics in vivo conditions. Results To identify the optimal medium for coculture experiments, MRS broth and NYC-III broth were compared. NYC-III significantly enhanced the growth of G. vaginalis and four of the five tested Lactobacillus strains. We then developed a direct quantitative PCR (qPCR) method that allowed us to specifically measure G. vaginalis genome concentrations in cocultures with Lactobacillus. This direct qPCR did not require DNA extraction and had a 4,096-fold dynamic range. We then assessed the inhibition of G. vaginalis growth by Lactobacillus cell-free supernatants in G. vaginalis cultures by measuring optical density and the Lactobacillus-mediated inhibition in cocultures by the G. vaginalis specific direct qPCR. The two measurement methods showed different levels of inhibitory activity for two of the five Lactobacillus strains tested. These findings suggest that coculture experiments should be conducted in place of, or in addition to, supernatant-based inhibitory assays.https://doi.org/10.1186/s13104-025-07414-wLactobacillusGardnerellaVaginosisDNADirectqPCR |
| spellingShingle | Ferenc Somogyvári Nándor Tűzkő Attila Kereszturi László Párducz Mária Szécsényi Valéria Endrész Marianna Ábrók Caleb M. Ardizzone Katalin Burián Dezső Péter Virok Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis BMC Research Notes Lactobacillus Gardnerella Vaginosis DNA Direct qPCR |
| title | Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis |
| title_full | Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis |
| title_fullStr | Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis |
| title_full_unstemmed | Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis |
| title_short | Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis |
| title_sort | comparison of the inhibitory effects of lactobacillus supernatant and coculture on gardnerella vaginalis |
| topic | Lactobacillus Gardnerella Vaginosis DNA Direct qPCR |
| url | https://doi.org/10.1186/s13104-025-07414-w |
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