Red Cotton Stamen Extracts Mitigate Ferrous Sulfate-Induced Oxidative Stress and Enhance Quality in Bull Frozen Semen
Infertility is a significant global health concern, and incorporating antioxidants into sperm preparation media is one strategy to enhance sperm quality and decrease infertility rates. This study aimed to investigate the phytochemical compounds of red cotton stamen extracts and their effects as anti...
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MDPI AG
2025-07-01
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| Series: | Veterinary Sciences |
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| author | Jiraporn Laoung-on Jakree Jitjumnong Paiwan Sudwan Nopparuj Outaitaveep Sakaewan Ounjaijean Kongsak Boonyapranai |
| author_facet | Jiraporn Laoung-on Jakree Jitjumnong Paiwan Sudwan Nopparuj Outaitaveep Sakaewan Ounjaijean Kongsak Boonyapranai |
| author_sort | Jiraporn Laoung-on |
| collection | DOAJ |
| description | Infertility is a significant global health concern, and incorporating antioxidants into sperm preparation media is one strategy to enhance sperm quality and decrease infertility rates. This study aimed to investigate the phytochemical compounds of red cotton stamen extracts and their effects as antioxidants in improving the quality of bull frozen semen. Among the extracts, RCU contained the highest levels of total phenolics, total tannins, and total monomeric anthocyanins along with the strongest ABTS free radical scavenging activity and protein denaturation inhibition. Exposing sperm to FeSO<sub>4</sub>-induced oxidative stress resulted in significantly reduced motility, viability, and normal morphology. However, treatment with RCD, RCU, and RCM improved these parameters. Additionally, the FeSO<sub>4</sub>-induced group showed elevated levels of reactive oxygen species (ROS) and advanced glycation end products (AGEs) compared to the normal control, whereas all red cotton stamen extracts effectively reduced these levels. In conclusion, red cotton stamen extracts, rich in phenolic bioactive compounds, demonstrated strong free radical scavenging capacity and improved sperm motility, viability, and morphology by neutralizing free radicals and enhancing antioxidant defenses. These findings suggest that the red cotton stamen extracts, particularly RCD and RCU, offer benefits for sperm preservation. |
| format | Article |
| id | doaj-art-5e2f90fe1dc345cb9dc4c0426ed7e563 |
| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-5e2f90fe1dc345cb9dc4c0426ed7e5632025-08-20T03:32:19ZengMDPI AGVeterinary Sciences2306-73812025-07-0112767410.3390/vetsci12070674Red Cotton Stamen Extracts Mitigate Ferrous Sulfate-Induced Oxidative Stress and Enhance Quality in Bull Frozen SemenJiraporn Laoung-on0Jakree Jitjumnong1Paiwan Sudwan2Nopparuj Outaitaveep3Sakaewan Ounjaijean4Kongsak Boonyapranai5Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandResearch Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandResearch Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandResearch Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandInfertility is a significant global health concern, and incorporating antioxidants into sperm preparation media is one strategy to enhance sperm quality and decrease infertility rates. This study aimed to investigate the phytochemical compounds of red cotton stamen extracts and their effects as antioxidants in improving the quality of bull frozen semen. Among the extracts, RCU contained the highest levels of total phenolics, total tannins, and total monomeric anthocyanins along with the strongest ABTS free radical scavenging activity and protein denaturation inhibition. Exposing sperm to FeSO<sub>4</sub>-induced oxidative stress resulted in significantly reduced motility, viability, and normal morphology. However, treatment with RCD, RCU, and RCM improved these parameters. Additionally, the FeSO<sub>4</sub>-induced group showed elevated levels of reactive oxygen species (ROS) and advanced glycation end products (AGEs) compared to the normal control, whereas all red cotton stamen extracts effectively reduced these levels. In conclusion, red cotton stamen extracts, rich in phenolic bioactive compounds, demonstrated strong free radical scavenging capacity and improved sperm motility, viability, and morphology by neutralizing free radicals and enhancing antioxidant defenses. These findings suggest that the red cotton stamen extracts, particularly RCD and RCU, offer benefits for sperm preservation.https://www.mdpi.com/2306-7381/12/7/674ROS<i>Bombax ceiba</i>sperm motilitysperm viabilityphytochemicals |
| spellingShingle | Jiraporn Laoung-on Jakree Jitjumnong Paiwan Sudwan Nopparuj Outaitaveep Sakaewan Ounjaijean Kongsak Boonyapranai Red Cotton Stamen Extracts Mitigate Ferrous Sulfate-Induced Oxidative Stress and Enhance Quality in Bull Frozen Semen Veterinary Sciences ROS <i>Bombax ceiba</i> sperm motility sperm viability phytochemicals |
| title | Red Cotton Stamen Extracts Mitigate Ferrous Sulfate-Induced Oxidative Stress and Enhance Quality in Bull Frozen Semen |
| title_full | Red Cotton Stamen Extracts Mitigate Ferrous Sulfate-Induced Oxidative Stress and Enhance Quality in Bull Frozen Semen |
| title_fullStr | Red Cotton Stamen Extracts Mitigate Ferrous Sulfate-Induced Oxidative Stress and Enhance Quality in Bull Frozen Semen |
| title_full_unstemmed | Red Cotton Stamen Extracts Mitigate Ferrous Sulfate-Induced Oxidative Stress and Enhance Quality in Bull Frozen Semen |
| title_short | Red Cotton Stamen Extracts Mitigate Ferrous Sulfate-Induced Oxidative Stress and Enhance Quality in Bull Frozen Semen |
| title_sort | red cotton stamen extracts mitigate ferrous sulfate induced oxidative stress and enhance quality in bull frozen semen |
| topic | ROS <i>Bombax ceiba</i> sperm motility sperm viability phytochemicals |
| url | https://www.mdpi.com/2306-7381/12/7/674 |
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