ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS
This study was conducted to evaluate the efficiency of some PGPR isolates as biological agents in controling root rot disease (Rhizoctonia in broad beans) caused by the fungus Rhizoctonia solani. Tweenty six isolates were obtained through the Plant Growth Promoting Rhizobacteria sampling from differ...
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University of Anbar
2025-06-01
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| Series: | مجلة الأنبار للعلوم الزراعية |
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| Online Access: | https://ajas.uoanbar.edu.iq/article_186566_87987699e12ec6161671b6239919e689.pdf |
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| author | S. H. A. AL-Mayahi A. K. Hassan S. A. H. Alamery |
| author_facet | S. H. A. AL-Mayahi A. K. Hassan S. A. H. Alamery |
| author_sort | S. H. A. AL-Mayahi |
| collection | DOAJ |
| description | This study was conducted to evaluate the efficiency of some PGPR isolates as biological agents in controling root rot disease (Rhizoctonia in broad beans) caused by the fungus Rhizoctonia solani. Tweenty six isolates were obtained through the Plant Growth Promoting Rhizobacteria sampling from different areas. Two isolates demonstrated a high antagonistic activity against the pathogenic fungus isolate. The identification of two isolates were Alcaligenes faecalis and Acinetobacter radioresistens was confirmed using vitek technology and nucleotide sequencing. The two strain sequences were deposited in the Gen Bank under accession numbers AR1 (OR101916) and AR2 (OR101917). The antagonistic activity was 100% for both isolates in the first method according to (Al-Mayahi and Hassan, 2021). While in the second method according to (Raspor at all, 2010), the two isolates of A. faecalis and A. radioresistens scored 58.92 and 57.14% inhibition at concentration 10-7 and 10-6, respectively. Secondary metabolites test of A. faecalis and A. radioresistens scored 93.33 and 85.55% inhibition rate at a concentration 10% |
| format | Article |
| id | doaj-art-5e26b1ab3c0049e5995dcd71d1cd5c4a |
| institution | Kabale University |
| issn | 1992-7479 2617-6211 |
| language | Arabic |
| publishDate | 2025-06-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة الأنبار للعلوم الزراعية |
| spelling | doaj-art-5e26b1ab3c0049e5995dcd71d1cd5c4a2025-08-20T03:33:34ZaraUniversity of Anbarمجلة الأنبار للعلوم الزراعية1992-74792617-62112025-06-01231779210.32649/ajas.2025.186566186566ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONSS. H. A. AL-Mayahi0A. K. Hassan1S. A. H. Alamery2Dep Diagnostic, Plants Prot Office, Ministry Agric, BaghdadDept. Plant Prot., Coll. of Agric. Engin. Sci., University of BaghdadDep Diagnostic, Plants Prot Office, Ministry Agric, BaghdadThis study was conducted to evaluate the efficiency of some PGPR isolates as biological agents in controling root rot disease (Rhizoctonia in broad beans) caused by the fungus Rhizoctonia solani. Tweenty six isolates were obtained through the Plant Growth Promoting Rhizobacteria sampling from different areas. Two isolates demonstrated a high antagonistic activity against the pathogenic fungus isolate. The identification of two isolates were Alcaligenes faecalis and Acinetobacter radioresistens was confirmed using vitek technology and nucleotide sequencing. The two strain sequences were deposited in the Gen Bank under accession numbers AR1 (OR101916) and AR2 (OR101917). The antagonistic activity was 100% for both isolates in the first method according to (Al-Mayahi and Hassan, 2021). While in the second method according to (Raspor at all, 2010), the two isolates of A. faecalis and A. radioresistens scored 58.92 and 57.14% inhibition at concentration 10-7 and 10-6, respectively. Secondary metabolites test of A. faecalis and A. radioresistens scored 93.33 and 85.55% inhibition rate at a concentration 10%https://ajas.uoanbar.edu.iq/article_186566_87987699e12ec6161671b6239919e689.pdfbroad beanr. solanipgpra. faecalisa. radioresistens |
| spellingShingle | S. H. A. AL-Mayahi A. K. Hassan S. A. H. Alamery ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS مجلة الأنبار للعلوم الزراعية broad bean r. solani pgpr a. faecalis a. radioresistens |
| title | ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS |
| title_full | ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS |
| title_fullStr | ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS |
| title_full_unstemmed | ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS |
| title_short | ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS |
| title_sort | isolation and identification of local pgpr isolates and evaluation of their efficiency in inhibiting the pathogenic fungus rhizoctonia solani on broad bean in laboratory conditions |
| topic | broad bean r. solani pgpr a. faecalis a. radioresistens |
| url | https://ajas.uoanbar.edu.iq/article_186566_87987699e12ec6161671b6239919e689.pdf |
| work_keys_str_mv | AT shaalmayahi isolationandidentificationoflocalpgprisolatesandevaluationoftheirefficiencyininhibitingthepathogenicfungusrhizoctoniasolanionbroadbeaninlaboratoryconditions AT akhassan isolationandidentificationoflocalpgprisolatesandevaluationoftheirefficiencyininhibitingthepathogenicfungusrhizoctoniasolanionbroadbeaninlaboratoryconditions AT sahalamery isolationandidentificationoflocalpgprisolatesandevaluationoftheirefficiencyininhibitingthepathogenicfungusrhizoctoniasolanionbroadbeaninlaboratoryconditions |