Synergistic Enhancement of Cauliflower Yield: Harnessing Phosphate-Solubilizing Bacteria and Nitrogen-Fixing Microbes for Sustainable Agriculture

Cauliflower (Brassica oleracea var. botrytis) is a crucial cash crop predominantly consumed as a vegetable. High-yielding varieties are favored to maximize productivity and income, but they require substantial nutrients, leading to heavy reliance on chemical fertilizers and pesticides. This practice...

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Main Authors: Parmeshwar Singh, Anju Meshram, Ravi Kant Singh, Laiq ur Rahman, Vivek Kumar Morya
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2024-12-01
Series:Journal of Pure and Applied Microbiology
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Online Access:https://microbiologyjournal.org/synergistic-enhancement-of-cauliflower-yield-harnessing-phosphate-solubilizing-bacteria-and-nitrogen-fixing-microbes-for-sustainable-agriculture/
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author Parmeshwar Singh
Anju Meshram
Ravi Kant Singh
Laiq ur Rahman
Vivek Kumar Morya
author_facet Parmeshwar Singh
Anju Meshram
Ravi Kant Singh
Laiq ur Rahman
Vivek Kumar Morya
author_sort Parmeshwar Singh
collection DOAJ
description Cauliflower (Brassica oleracea var. botrytis) is a crucial cash crop predominantly consumed as a vegetable. High-yielding varieties are favored to maximize productivity and income, but they require substantial nutrients, leading to heavy reliance on chemical fertilizers and pesticides. This practice poses health risks and causes environmental pollution. Adequate nutrient availability, particularly for phosphorus (P) and nitrogen (N), is essential for optimal cauliflower growth. Phosphate-solubilizing bacteria (PSB) enhance P availability by solubilizing insoluble phosphates, whereas nitrogen-fixing microbes (NFM) convert atmospheric nitrogen into usable forms. These microbial inoculants are eco-friendly alternatives to chemical fertilizers, which promote nutrient availability and plant growth. The purpose of this study was to separate, identify, and describe PSB from the soil of the cauliflower rhizosphere in the Uttar Pradesh district of Lucknow, Unnao, and Kanpur. Selected PSB isolates were screened, characterized using 16S rRNA, and evaluated for their phosphate solubilization capacity at different phosphorus concentrations. The results showed increased phosphate solubilization up to 72 h, with tricalcium phosphate (TCP) solubilized most effectively at 500 ppm and rock phosphate (RP) or bone meal (BM) at 250 ppm. Bacillus pumilus exhibited the highest phosphate solubilization ability. This research highlights the potential of PSB and NFM as sustainable solutions for reducing chemical fertilizer dependency, enhancing soil fertility, and promoting cauliflower growth, thereby offering a promising approach to sustainable agriculture.
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spelling doaj-art-5e12e100251343c2ab26d2eefb59b7a72025-08-20T02:22:06ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2024-12-0118425822591https://doi.org/10.22207/JPAM.18.4.31Synergistic Enhancement of Cauliflower Yield: Harnessing Phosphate-Solubilizing Bacteria and Nitrogen-Fixing Microbes for Sustainable AgricultureParmeshwar SinghAnju MeshramRavi Kant Singhhttps://orcid.org/0000-0003-3073-2010Laiq ur RahmanVivek Kumar MoryaCauliflower (Brassica oleracea var. botrytis) is a crucial cash crop predominantly consumed as a vegetable. High-yielding varieties are favored to maximize productivity and income, but they require substantial nutrients, leading to heavy reliance on chemical fertilizers and pesticides. This practice poses health risks and causes environmental pollution. Adequate nutrient availability, particularly for phosphorus (P) and nitrogen (N), is essential for optimal cauliflower growth. Phosphate-solubilizing bacteria (PSB) enhance P availability by solubilizing insoluble phosphates, whereas nitrogen-fixing microbes (NFM) convert atmospheric nitrogen into usable forms. These microbial inoculants are eco-friendly alternatives to chemical fertilizers, which promote nutrient availability and plant growth. The purpose of this study was to separate, identify, and describe PSB from the soil of the cauliflower rhizosphere in the Uttar Pradesh district of Lucknow, Unnao, and Kanpur. Selected PSB isolates were screened, characterized using 16S rRNA, and evaluated for their phosphate solubilization capacity at different phosphorus concentrations. The results showed increased phosphate solubilization up to 72 h, with tricalcium phosphate (TCP) solubilized most effectively at 500 ppm and rock phosphate (RP) or bone meal (BM) at 250 ppm. Bacillus pumilus exhibited the highest phosphate solubilization ability. This research highlights the potential of PSB and NFM as sustainable solutions for reducing chemical fertilizer dependency, enhancing soil fertility, and promoting cauliflower growth, thereby offering a promising approach to sustainable agriculture.https://microbiologyjournal.org/synergistic-enhancement-of-cauliflower-yield-harnessing-phosphate-solubilizing-bacteria-and-nitrogen-fixing-microbes-for-sustainable-agriculture/bacillus pumiluscauliflower (brassica oleracea var. botrytis)nitrogen-fixing microbes (nfm)phosphate-solubilizing bacteria (psb)rhizosphere16s rrna sequencing
spellingShingle Parmeshwar Singh
Anju Meshram
Ravi Kant Singh
Laiq ur Rahman
Vivek Kumar Morya
Synergistic Enhancement of Cauliflower Yield: Harnessing Phosphate-Solubilizing Bacteria and Nitrogen-Fixing Microbes for Sustainable Agriculture
Journal of Pure and Applied Microbiology
bacillus pumilus
cauliflower (brassica oleracea var. botrytis)
nitrogen-fixing microbes (nfm)
phosphate-solubilizing bacteria (psb)
rhizosphere
16s rrna sequencing
title Synergistic Enhancement of Cauliflower Yield: Harnessing Phosphate-Solubilizing Bacteria and Nitrogen-Fixing Microbes for Sustainable Agriculture
title_full Synergistic Enhancement of Cauliflower Yield: Harnessing Phosphate-Solubilizing Bacteria and Nitrogen-Fixing Microbes for Sustainable Agriculture
title_fullStr Synergistic Enhancement of Cauliflower Yield: Harnessing Phosphate-Solubilizing Bacteria and Nitrogen-Fixing Microbes for Sustainable Agriculture
title_full_unstemmed Synergistic Enhancement of Cauliflower Yield: Harnessing Phosphate-Solubilizing Bacteria and Nitrogen-Fixing Microbes for Sustainable Agriculture
title_short Synergistic Enhancement of Cauliflower Yield: Harnessing Phosphate-Solubilizing Bacteria and Nitrogen-Fixing Microbes for Sustainable Agriculture
title_sort synergistic enhancement of cauliflower yield harnessing phosphate solubilizing bacteria and nitrogen fixing microbes for sustainable agriculture
topic bacillus pumilus
cauliflower (brassica oleracea var. botrytis)
nitrogen-fixing microbes (nfm)
phosphate-solubilizing bacteria (psb)
rhizosphere
16s rrna sequencing
url https://microbiologyjournal.org/synergistic-enhancement-of-cauliflower-yield-harnessing-phosphate-solubilizing-bacteria-and-nitrogen-fixing-microbes-for-sustainable-agriculture/
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