Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits

IntroductionPost-weaning Diarrhea (PWD) is a kind of physiological stress diarrhea in Rex rabbits after weaning, which can lead to death in severe cases. Traditional Chinese medicine (TCM) has been widely used in animal due to its advantages of natural origin, diverse functions, safety, reliability,...

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Main Authors: Dongbo Li, Chao Li, Ning Liu, Hanzhong Liu, Zhiju Yu, Quanjin Liu, Gang Shu, Juchun Lin, Wei Zhang, Guangneng Peng, Ling Zhao, Huaqiao Tang, Haohuan Li, Funeng Xu, Hualin Fu
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Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1470731/full
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author Dongbo Li
Chao Li
Ning Liu
Hanzhong Liu
Zhiju Yu
Quanjin Liu
Gang Shu
Juchun Lin
Wei Zhang
Guangneng Peng
Ling Zhao
Huaqiao Tang
Haohuan Li
Funeng Xu
Hualin Fu
author_facet Dongbo Li
Chao Li
Ning Liu
Hanzhong Liu
Zhiju Yu
Quanjin Liu
Gang Shu
Juchun Lin
Wei Zhang
Guangneng Peng
Ling Zhao
Huaqiao Tang
Haohuan Li
Funeng Xu
Hualin Fu
author_sort Dongbo Li
collection DOAJ
description IntroductionPost-weaning Diarrhea (PWD) is a kind of physiological stress diarrhea in Rex rabbits after weaning, which can lead to death in severe cases. Traditional Chinese medicine (TCM) has been widely used in animal due to its advantages of natural origin, diverse functions, safety, reliability, economy and environmental protection. Modified Yupingfeng Granule (MYPFG) is an improved Yupingfeng prescription based on the famous traditional Chinese prescription Yupingfeng (YPF), which is combined with other TCM and has obvious synergistic and additive activity in order to obtain an excellent natural medicine for PWD.MethodsIn this study, 120 weaned Rex rabbits were randomly allocated to 4 treatment groups, including control (CON), low dose (LD), medium dose (MD), high dose (HD). Rabbits were fed a control diet or a different MYPFG proportions of diet for 30 days. The study combined 16S rRNA analysis of intestinal microbiota and cecal contents metabolomics to explore the MYPFG effect on weaned Rex rabbits.ResultsMYPFG increased average daily gain, villus length to crypt depth ratio and decreased the feed to meat ratio, diarrhea frequency, mortality rate, depth of crypt (p < 0.05). The intestinal microbiota test found that MYPFG could change the abundances of Patescibacteria, Sphingobium, Ruminococcus, and Oxalobacter. Metabolomics analysis found that effect may be related to its regulation of Glycine, serine and threonine metabolism, Arginine and proline metabolism. Nicotinate and nicotinamide metabolism.DiscussionMYPFG could regulate intestinal microbiota and change the metabolic pathway of some amino acids to alleviate the PWD in Rex rabbits.
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spelling doaj-art-5e0c388349014d729dacbbbbd1d41a5c2025-08-20T02:11:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-04-011610.3389/fmicb.2025.14707311470731Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbitsDongbo Li0Chao Li1Ning Liu2Hanzhong Liu3Zhiju Yu4Quanjin Liu5Gang Shu6Juchun Lin7Wei Zhang8Guangneng Peng9Ling Zhao10Huaqiao Tang11Haohuan Li12Funeng Xu13Hualin Fu14Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaSichuan Academy of Grassland Sciences, Chengdu, Sichuan, ChinaSichuan Academy of Grassland Sciences, Chengdu, Sichuan, ChinaSichuan Academy of Grassland Sciences, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, ChinaIntroductionPost-weaning Diarrhea (PWD) is a kind of physiological stress diarrhea in Rex rabbits after weaning, which can lead to death in severe cases. Traditional Chinese medicine (TCM) has been widely used in animal due to its advantages of natural origin, diverse functions, safety, reliability, economy and environmental protection. Modified Yupingfeng Granule (MYPFG) is an improved Yupingfeng prescription based on the famous traditional Chinese prescription Yupingfeng (YPF), which is combined with other TCM and has obvious synergistic and additive activity in order to obtain an excellent natural medicine for PWD.MethodsIn this study, 120 weaned Rex rabbits were randomly allocated to 4 treatment groups, including control (CON), low dose (LD), medium dose (MD), high dose (HD). Rabbits were fed a control diet or a different MYPFG proportions of diet for 30 days. The study combined 16S rRNA analysis of intestinal microbiota and cecal contents metabolomics to explore the MYPFG effect on weaned Rex rabbits.ResultsMYPFG increased average daily gain, villus length to crypt depth ratio and decreased the feed to meat ratio, diarrhea frequency, mortality rate, depth of crypt (p < 0.05). The intestinal microbiota test found that MYPFG could change the abundances of Patescibacteria, Sphingobium, Ruminococcus, and Oxalobacter. Metabolomics analysis found that effect may be related to its regulation of Glycine, serine and threonine metabolism, Arginine and proline metabolism. Nicotinate and nicotinamide metabolism.DiscussionMYPFG could regulate intestinal microbiota and change the metabolic pathway of some amino acids to alleviate the PWD in Rex rabbits.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1470731/fullModified Yupingfeng Granulepost-weaning diarrheaintestinal microbiotametabolomicsRex rabbits
spellingShingle Dongbo Li
Chao Li
Ning Liu
Hanzhong Liu
Zhiju Yu
Quanjin Liu
Gang Shu
Juchun Lin
Wei Zhang
Guangneng Peng
Ling Zhao
Huaqiao Tang
Haohuan Li
Funeng Xu
Hualin Fu
Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits
Frontiers in Microbiology
Modified Yupingfeng Granule
post-weaning diarrhea
intestinal microbiota
metabolomics
Rex rabbits
title Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits
title_full Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits
title_fullStr Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits
title_full_unstemmed Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits
title_short Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits
title_sort integrated metabolomics and intestinal microbiota analysis to reveal anti post weaning diarrhea mechanisms of modified yupingfeng granule in rex rabbits
topic Modified Yupingfeng Granule
post-weaning diarrhea
intestinal microbiota
metabolomics
Rex rabbits
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1470731/full
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