In vitro propagation of walnut varieties from Uttarakhand

The Persian walnut, or English walnut, is one of the most prized walnuts, popular for its timber, medicinal properties, oil, and nuts. Due to its high demand across the globe, walnut cultivation has exponentially grown over the years. Despite having the appropriate climate, the state of Uttarakhand...

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Main Authors: Sakshi Juyal, Aarushi Gautam, Manu Pant
Format: Article
Language:English
Published: Action for Sustainable Efficacious Development and Awareness 2024-11-01
Series:Environment Conservation Journal
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Online Access:https://journal.environcj.in/index.php/ecj/article/view/3057
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author Sakshi Juyal
Aarushi Gautam
Manu Pant
author_facet Sakshi Juyal
Aarushi Gautam
Manu Pant
author_sort Sakshi Juyal
collection DOAJ
description The Persian walnut, or English walnut, is one of the most prized walnuts, popular for its timber, medicinal properties, oil, and nuts. Due to its high demand across the globe, walnut cultivation has exponentially grown over the years. Despite having the appropriate climate, the state of Uttarakhand still does not cultivate walnut on a large scale. The alternative to growing walnuts with better quality and high production is through in vitro propagation. The present study was conducted to propagate walnut varieties growing in the Uttarakhand region through direct and indirect organogenesis. The DKW medium proved to be the most effective for nodal explant culture, while the MS medium yielded superior results for callus induction. We also optimized the media by varying the liquid/semisolid medium and plant growth regulator combinations. The genotype from higher altitudes of Uttarakhand was found to respond better to the in vitro treatments. The findings can prove useful in propagating good-quality walnut varieties in Uttarakhand on a large scale.
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publishDate 2024-11-01
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spelling doaj-art-5e09c4ee2a5e4cf09d722938149611712025-08-20T01:57:05ZengAction for Sustainable Efficacious Development and AwarenessEnvironment Conservation Journal0972-30992278-51242024-11-0125410.36953/ECJ.30570524In vitro propagation of walnut varieties from UttarakhandSakshi Juyal0Aarushi Gautam1Manu Pant2Department of Life Sciences, Graphic Era Deemed to be University, Dehradun, UttarakhandDepartment of Biotechnology, Graphic Era Deemed to be University, Dehradun, UttarakhandDepartment of Biotechnology, Graphic Era Deemed to be University, Dehradun, Uttarakhand The Persian walnut, or English walnut, is one of the most prized walnuts, popular for its timber, medicinal properties, oil, and nuts. Due to its high demand across the globe, walnut cultivation has exponentially grown over the years. Despite having the appropriate climate, the state of Uttarakhand still does not cultivate walnut on a large scale. The alternative to growing walnuts with better quality and high production is through in vitro propagation. The present study was conducted to propagate walnut varieties growing in the Uttarakhand region through direct and indirect organogenesis. The DKW medium proved to be the most effective for nodal explant culture, while the MS medium yielded superior results for callus induction. We also optimized the media by varying the liquid/semisolid medium and plant growth regulator combinations. The genotype from higher altitudes of Uttarakhand was found to respond better to the in vitro treatments. The findings can prove useful in propagating good-quality walnut varieties in Uttarakhand on a large scale. https://journal.environcj.in/index.php/ecj/article/view/3057Callus cultureCommercial valueMedicinal valueMass propagationNodal explant cultureWalnut
spellingShingle Sakshi Juyal
Aarushi Gautam
Manu Pant
In vitro propagation of walnut varieties from Uttarakhand
Environment Conservation Journal
Callus culture
Commercial value
Medicinal value
Mass propagation
Nodal explant culture
Walnut
title In vitro propagation of walnut varieties from Uttarakhand
title_full In vitro propagation of walnut varieties from Uttarakhand
title_fullStr In vitro propagation of walnut varieties from Uttarakhand
title_full_unstemmed In vitro propagation of walnut varieties from Uttarakhand
title_short In vitro propagation of walnut varieties from Uttarakhand
title_sort in vitro propagation of walnut varieties from uttarakhand
topic Callus culture
Commercial value
Medicinal value
Mass propagation
Nodal explant culture
Walnut
url https://journal.environcj.in/index.php/ecj/article/view/3057
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AT aarushigautam invitropropagationofwalnutvarietiesfromuttarakhand
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