The Bisulphite-Sequencing PCR for the Bovine Gstp1, Gsta4, and Ache Gene Methylation Study
Modifying cytosine by adding a methyl group in CpG dinucleotides within DNA sequence has several functions, but acts primarily as an important regulatory mechanism of mammalian gene expression. This consequently affects the phenotypic characteristics of the organism. The methylation profiles can be...
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| Format: | Article |
| Language: | English |
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Sciendo
2024-12-01
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| Series: | Folia Veterinaria |
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| Online Access: | https://doi.org/10.2478/fv-2024-0037 |
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| author | Halušková Jana Holečková Beáta Košuth Ján Galdíková Martina Schwarzbacherová Viera |
| author_facet | Halušková Jana Holečková Beáta Košuth Ján Galdíková Martina Schwarzbacherová Viera |
| author_sort | Halušková Jana |
| collection | DOAJ |
| description | Modifying cytosine by adding a methyl group in CpG dinucleotides within DNA sequence has several functions, but acts primarily as an important regulatory mechanism of mammalian gene expression. This consequently affects the phenotypic characteristics of the organism. The methylation profiles can be altered as a consequence of various environmental influences, age, disease, or diet, and can serve as biomarkers of important production and health traits. In this regard, the methylation profile analysis techniques are important. In the present study, we proposed and tested specific oligonucleotide primers for the bisulphite-sequencing polymerase chain reaction (BSP) analysis of the bovine GSTP1, GSTA4, and AChE genes, to optimize this approach for future studies of the methylation status of these genes. BSP can be used, for example, to study the effect of different pesticides and their combinations on the methylation of these and other bovine genes. |
| format | Article |
| id | doaj-art-5e0186928dca461d896813b4f925f09a |
| institution | DOAJ |
| issn | 2453-7837 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Folia Veterinaria |
| spelling | doaj-art-5e0186928dca461d896813b4f925f09a2025-08-20T02:51:48ZengSciendoFolia Veterinaria2453-78372024-12-01684434710.2478/fv-2024-0037The Bisulphite-Sequencing PCR for the Bovine Gstp1, Gsta4, and Ache Gene Methylation StudyHalušková Jana0Holečková Beáta1Košuth Ján2Galdíková Martina3Schwarzbacherová Viera41Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice1Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice2Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in KošiceSlovakia1Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice1Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in KošiceModifying cytosine by adding a methyl group in CpG dinucleotides within DNA sequence has several functions, but acts primarily as an important regulatory mechanism of mammalian gene expression. This consequently affects the phenotypic characteristics of the organism. The methylation profiles can be altered as a consequence of various environmental influences, age, disease, or diet, and can serve as biomarkers of important production and health traits. In this regard, the methylation profile analysis techniques are important. In the present study, we proposed and tested specific oligonucleotide primers for the bisulphite-sequencing polymerase chain reaction (BSP) analysis of the bovine GSTP1, GSTA4, and AChE genes, to optimize this approach for future studies of the methylation status of these genes. BSP can be used, for example, to study the effect of different pesticides and their combinations on the methylation of these and other bovine genes.https://doi.org/10.2478/fv-2024-0037acetylcholinesterasebisulphite sequencingbovinesdna methylationglutathione-s-transferase a4glutathione-s-transferase p1primer |
| spellingShingle | Halušková Jana Holečková Beáta Košuth Ján Galdíková Martina Schwarzbacherová Viera The Bisulphite-Sequencing PCR for the Bovine Gstp1, Gsta4, and Ache Gene Methylation Study Folia Veterinaria acetylcholinesterase bisulphite sequencing bovines dna methylation glutathione-s-transferase a4 glutathione-s-transferase p1 primer |
| title | The Bisulphite-Sequencing PCR for the Bovine Gstp1, Gsta4, and Ache Gene Methylation Study |
| title_full | The Bisulphite-Sequencing PCR for the Bovine Gstp1, Gsta4, and Ache Gene Methylation Study |
| title_fullStr | The Bisulphite-Sequencing PCR for the Bovine Gstp1, Gsta4, and Ache Gene Methylation Study |
| title_full_unstemmed | The Bisulphite-Sequencing PCR for the Bovine Gstp1, Gsta4, and Ache Gene Methylation Study |
| title_short | The Bisulphite-Sequencing PCR for the Bovine Gstp1, Gsta4, and Ache Gene Methylation Study |
| title_sort | bisulphite sequencing pcr for the bovine gstp1 gsta4 and ache gene methylation study |
| topic | acetylcholinesterase bisulphite sequencing bovines dna methylation glutathione-s-transferase a4 glutathione-s-transferase p1 primer |
| url | https://doi.org/10.2478/fv-2024-0037 |
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