Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy
Wharton’s jelly mesenchymal stem cells (WJ-MSC) exhibit immunomodulatory effects on T cell response. WJ-MSC are easy to collect, process, and proliferate rapidly in culture, but information on the variability of individual cell samples impacting upon in vitro expansion, immunomodulatory potential, a...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/8492797 |
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| _version_ | 1849411508330561536 |
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| author | Fernanda Vieira Paladino Luiz Roberto Sardinha Carla Azevedo Piccinato Anna Carla Goldberg |
| author_facet | Fernanda Vieira Paladino Luiz Roberto Sardinha Carla Azevedo Piccinato Anna Carla Goldberg |
| author_sort | Fernanda Vieira Paladino |
| collection | DOAJ |
| description | Wharton’s jelly mesenchymal stem cells (WJ-MSC) exhibit immunomodulatory effects on T cell response. WJ-MSC are easy to collect, process, and proliferate rapidly in culture, but information on the variability of individual cell samples impacting upon in vitro expansion, immunomodulatory potential, and aging processes is still lacking. We propose to evaluate the immunomodulatory cytokine profile and capacity to inhibit T cell proliferation of WJ-MSC progressing to replicative senescence in order to analyze if expected responses are affected. Our results show that the gene expression of immunomodulatory molecules varied among samples with no specific pattern present. In coculture, all WJ-MSC were capable of inhibiting mitogen-activated CD3+ T cell proliferation, although to different degrees, and each PBMC responded with a different level of inhibition. Thus, we suggest that each WJ-MSC displays unique behavior, differing in patterns of cytokine mRNA expression and immunomodulatory capacity. We believe that variability between samples may play a role in the effectiveness of WJ-MSC employed therapeutically. |
| format | Article |
| id | doaj-art-5df01698d9d14819b29854aff3c5ec3c |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5df01698d9d14819b29854aff3c5ec3c2025-08-20T03:34:45ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/84927978492797Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell TherapyFernanda Vieira Paladino0Luiz Roberto Sardinha1Carla Azevedo Piccinato2Anna Carla Goldberg3Hospital Israelita Albert Einstein, São Paulo, SP, BrazilHospital Israelita Albert Einstein, São Paulo, SP, BrazilHospital Israelita Albert Einstein, São Paulo, SP, BrazilHospital Israelita Albert Einstein, São Paulo, SP, BrazilWharton’s jelly mesenchymal stem cells (WJ-MSC) exhibit immunomodulatory effects on T cell response. WJ-MSC are easy to collect, process, and proliferate rapidly in culture, but information on the variability of individual cell samples impacting upon in vitro expansion, immunomodulatory potential, and aging processes is still lacking. We propose to evaluate the immunomodulatory cytokine profile and capacity to inhibit T cell proliferation of WJ-MSC progressing to replicative senescence in order to analyze if expected responses are affected. Our results show that the gene expression of immunomodulatory molecules varied among samples with no specific pattern present. In coculture, all WJ-MSC were capable of inhibiting mitogen-activated CD3+ T cell proliferation, although to different degrees, and each PBMC responded with a different level of inhibition. Thus, we suggest that each WJ-MSC displays unique behavior, differing in patterns of cytokine mRNA expression and immunomodulatory capacity. We believe that variability between samples may play a role in the effectiveness of WJ-MSC employed therapeutically.http://dx.doi.org/10.1155/2017/8492797 |
| spellingShingle | Fernanda Vieira Paladino Luiz Roberto Sardinha Carla Azevedo Piccinato Anna Carla Goldberg Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy Stem Cells International |
| title | Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy |
| title_full | Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy |
| title_fullStr | Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy |
| title_full_unstemmed | Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy |
| title_short | Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy |
| title_sort | intrinsic variability present in wharton s jelly mesenchymal stem cells and t cell responses may impact cell therapy |
| url | http://dx.doi.org/10.1155/2017/8492797 |
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