Organotypic Cultures as a Model to Study Adult Neurogenesis in CNS Disorders
Neural regeneration resides in certain specific regions of adult CNS. Adult neurogenesis occurs throughout life, especially from the subgranular zone of hippocampus and the subventricular zone, and can be modulated in physiological and pathological conditions. Numerous techniques and animal models h...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/3540568 |
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| author | Fabio Cavaliere Monica Benito-Muñoz Carlos Matute |
| author_facet | Fabio Cavaliere Monica Benito-Muñoz Carlos Matute |
| author_sort | Fabio Cavaliere |
| collection | DOAJ |
| description | Neural regeneration resides in certain specific regions of adult CNS. Adult neurogenesis occurs throughout life, especially from the subgranular zone of hippocampus and the subventricular zone, and can be modulated in physiological and pathological conditions. Numerous techniques and animal models have been developed to demonstrate and observe neural regeneration but, in order to study the molecular and cellular mechanisms and to characterize multiple types of cell populations involved in the activation of neurogenesis and gliogenesis, investigators have to turn to in vitro models. Organotypic cultures best recapitulate the 3D organization of the CNS and can be explored taking advantage of many techniques. Here, we review the use of organotypic cultures as a reliable and well defined method to study the mechanisms of neurogenesis under normal and pathological conditions. As an example, we will focus on the possibilities these cultures offer to study the pathophysiology of diseases like Alzheimer disease, Parkinson’s disease, and cerebral ischemia. |
| format | Article |
| id | doaj-art-5deeb5ab1a6b4b8d97b730910be0fae0 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5deeb5ab1a6b4b8d97b730910be0fae02025-08-20T02:06:03ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/35405683540568Organotypic Cultures as a Model to Study Adult Neurogenesis in CNS DisordersFabio Cavaliere0Monica Benito-Muñoz1Carlos Matute2Departamento de Neurociencias, Achucarro Basque Center for Neuroscience, Universidad del País Vasco (UPV/EHU), and Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), 48940 Leioa, SpainDepartamento de Neurociencias, Achucarro Basque Center for Neuroscience, Universidad del País Vasco (UPV/EHU), and Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), 48940 Leioa, SpainDepartamento de Neurociencias, Achucarro Basque Center for Neuroscience, Universidad del País Vasco (UPV/EHU), and Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), 48940 Leioa, SpainNeural regeneration resides in certain specific regions of adult CNS. Adult neurogenesis occurs throughout life, especially from the subgranular zone of hippocampus and the subventricular zone, and can be modulated in physiological and pathological conditions. Numerous techniques and animal models have been developed to demonstrate and observe neural regeneration but, in order to study the molecular and cellular mechanisms and to characterize multiple types of cell populations involved in the activation of neurogenesis and gliogenesis, investigators have to turn to in vitro models. Organotypic cultures best recapitulate the 3D organization of the CNS and can be explored taking advantage of many techniques. Here, we review the use of organotypic cultures as a reliable and well defined method to study the mechanisms of neurogenesis under normal and pathological conditions. As an example, we will focus on the possibilities these cultures offer to study the pathophysiology of diseases like Alzheimer disease, Parkinson’s disease, and cerebral ischemia.http://dx.doi.org/10.1155/2016/3540568 |
| spellingShingle | Fabio Cavaliere Monica Benito-Muñoz Carlos Matute Organotypic Cultures as a Model to Study Adult Neurogenesis in CNS Disorders Stem Cells International |
| title | Organotypic Cultures as a Model to Study Adult Neurogenesis in CNS Disorders |
| title_full | Organotypic Cultures as a Model to Study Adult Neurogenesis in CNS Disorders |
| title_fullStr | Organotypic Cultures as a Model to Study Adult Neurogenesis in CNS Disorders |
| title_full_unstemmed | Organotypic Cultures as a Model to Study Adult Neurogenesis in CNS Disorders |
| title_short | Organotypic Cultures as a Model to Study Adult Neurogenesis in CNS Disorders |
| title_sort | organotypic cultures as a model to study adult neurogenesis in cns disorders |
| url | http://dx.doi.org/10.1155/2016/3540568 |
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