High-capacity information storage using peptide-encapsulated hydrogels for long-term data preservation

Abstract Peptide-based data storage offers a promising solution to the escalating digital data crisis. A key challenge is maintaining peptide stability over time after encoding information in amino acid sequences. Here, we present a simple and effective method to preserve peptides using a hydrogel c...

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Main Authors: Benxiang Luo, Songyan Gao, Minghao Wu, Xin Dong, Xiaoyong Deng, Honggang Hu
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Communications Materials
Online Access:https://doi.org/10.1038/s43246-025-00915-y
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author Benxiang Luo
Songyan Gao
Minghao Wu
Xin Dong
Xiaoyong Deng
Honggang Hu
author_facet Benxiang Luo
Songyan Gao
Minghao Wu
Xin Dong
Xiaoyong Deng
Honggang Hu
author_sort Benxiang Luo
collection DOAJ
description Abstract Peptide-based data storage offers a promising solution to the escalating digital data crisis. A key challenge is maintaining peptide stability over time after encoding information in amino acid sequences. Here, we present a simple and effective method to preserve peptides using a hydrogel composed of poly(N-isopropylacrylamide) and chitosan that forms a semi-interpenetrating polymer network. This hydrogel responds to temperature and pH stimuli to encapsulate and stabilize peptides from solution. Its thermoresponsive behavior enables efficient peptide concentration through repeated swelling and deswelling cycles. Strong electrostatic interactions between chitosan and peptides result in an ultrahigh data density of 2.44 × 1010 GB g−1. Accelerated aging tests under thermal and enzymatic conditions demonstrate significantly enhanced peptide stability, with original information fully recovered after 3.5 days at 70 °C, equivalent to over 600 years at 9.4 °C. This work establishes a durable, high-capacity platform for long-term peptide data storage.
format Article
id doaj-art-5deacb76e44c449fabe65ce39e42acee
institution Kabale University
issn 2662-4443
language English
publishDate 2025-08-01
publisher Nature Portfolio
record_format Article
series Communications Materials
spelling doaj-art-5deacb76e44c449fabe65ce39e42acee2025-08-24T11:40:47ZengNature PortfolioCommunications Materials2662-44432025-08-016111110.1038/s43246-025-00915-yHigh-capacity information storage using peptide-encapsulated hydrogels for long-term data preservationBenxiang Luo0Songyan Gao1Minghao Wu2Xin Dong3Xiaoyong Deng4Honggang Hu5Institute of Nanochemistry and Nanobiology, School of Environmental and Chemical Engineering, Shanghai UniversityInstitute of Translational Medicine, Shanghai UniversityInstitute of Translational Medicine, Shanghai UniversitySchool of Medicine, Shanghai UniversityInstitute of Nanochemistry and Nanobiology, School of Environmental and Chemical Engineering, Shanghai UniversitySchool of Medicine, Shanghai UniversityAbstract Peptide-based data storage offers a promising solution to the escalating digital data crisis. A key challenge is maintaining peptide stability over time after encoding information in amino acid sequences. Here, we present a simple and effective method to preserve peptides using a hydrogel composed of poly(N-isopropylacrylamide) and chitosan that forms a semi-interpenetrating polymer network. This hydrogel responds to temperature and pH stimuli to encapsulate and stabilize peptides from solution. Its thermoresponsive behavior enables efficient peptide concentration through repeated swelling and deswelling cycles. Strong electrostatic interactions between chitosan and peptides result in an ultrahigh data density of 2.44 × 1010 GB g−1. Accelerated aging tests under thermal and enzymatic conditions demonstrate significantly enhanced peptide stability, with original information fully recovered after 3.5 days at 70 °C, equivalent to over 600 years at 9.4 °C. This work establishes a durable, high-capacity platform for long-term peptide data storage.https://doi.org/10.1038/s43246-025-00915-y
spellingShingle Benxiang Luo
Songyan Gao
Minghao Wu
Xin Dong
Xiaoyong Deng
Honggang Hu
High-capacity information storage using peptide-encapsulated hydrogels for long-term data preservation
Communications Materials
title High-capacity information storage using peptide-encapsulated hydrogels for long-term data preservation
title_full High-capacity information storage using peptide-encapsulated hydrogels for long-term data preservation
title_fullStr High-capacity information storage using peptide-encapsulated hydrogels for long-term data preservation
title_full_unstemmed High-capacity information storage using peptide-encapsulated hydrogels for long-term data preservation
title_short High-capacity information storage using peptide-encapsulated hydrogels for long-term data preservation
title_sort high capacity information storage using peptide encapsulated hydrogels for long term data preservation
url https://doi.org/10.1038/s43246-025-00915-y
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