Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy Cows
The use of recycled manure solids (RMS) as cow bedding in dairy farms poses concerns due to its potential to harbor pathogenic and antimicrobial-resistant bacteria. This study evaluated the impact of RMS supplementation with biochar at three concentrations (2.5%, 5%, and 10%) on bacterial counts and...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/12/1/43 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587355165294592 |
|---|---|
| author | Ana José Pires Ana Filipa Esteves Gonçalo Pereira Catarina Geraldes Joana Fernandes Guerreiro Raquel Abreu Rita Magalhães Lélia Chambel Elisabete Silva David Fangueiro Manuela Oliveira Ricardo Bexiga |
| author_facet | Ana José Pires Ana Filipa Esteves Gonçalo Pereira Catarina Geraldes Joana Fernandes Guerreiro Raquel Abreu Rita Magalhães Lélia Chambel Elisabete Silva David Fangueiro Manuela Oliveira Ricardo Bexiga |
| author_sort | Ana José Pires |
| collection | DOAJ |
| description | The use of recycled manure solids (RMS) as cow bedding in dairy farms poses concerns due to its potential to harbor pathogenic and antimicrobial-resistant bacteria. This study evaluated the impact of RMS supplementation with biochar at three concentrations (2.5%, 5%, and 10%) on bacterial counts and on the antimicrobial resistance and virulence profiles of <i>Escherichia coli</i> and <i>Enterococcus</i> isolates. The results show that biochar supplementation did not promote a significant reduction in bacterial numbers. Overall, there were no significant changes in the isolates’ virulence or resistance profiles, and none of the isolates obtained were classified as high or moderate threats based on their MAR and VIR indexes. The most pathogenic <i>Enterococcus</i> isolates found were present in the control samples. A phylogenetic analysis of <i>E. coli</i> isolates allowed us to identify phylogroup D strains, predominantly in RMS supplemented with 2.5% and 10% biochar, which are associated with higher virulence and resistance. These findings indicate that the biochar concentrations tested were not effective in significantly reducing the bacterial risks associated with RMS. Further research is needed to evaluate different biochar formulations and concentrations, aiming to optimize its potential use for RMS supplementation. |
| format | Article |
| id | doaj-art-5dd48c50c0054eea8b7bc77969e1c467 |
| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-5dd48c50c0054eea8b7bc77969e1c4672025-01-24T13:52:05ZengMDPI AGVeterinary Sciences2306-73812025-01-011214310.3390/vetsci12010043Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy CowsAna José Pires0Ana Filipa Esteves1Gonçalo Pereira2Catarina Geraldes3Joana Fernandes Guerreiro4Raquel Abreu5Rita Magalhães6Lélia Chambel7Elisabete Silva8David Fangueiro9Manuela Oliveira10Ricardo Bexiga11Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalCentre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalCentre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalCentre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalCentre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalCentre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalCentre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalBiosystems and Integrative Sciences Institute (BioISI), Faculty of Sciences, University of Lisbon, 1741-016 Lisbon, PortugalCentre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalLEAF Research Center, Terra Associate Laboratory, Instituto Superior de Agronomia, University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, PortugalCentre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalCentre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica de Lisboa, 1300-477 Lisbon, PortugalThe use of recycled manure solids (RMS) as cow bedding in dairy farms poses concerns due to its potential to harbor pathogenic and antimicrobial-resistant bacteria. This study evaluated the impact of RMS supplementation with biochar at three concentrations (2.5%, 5%, and 10%) on bacterial counts and on the antimicrobial resistance and virulence profiles of <i>Escherichia coli</i> and <i>Enterococcus</i> isolates. The results show that biochar supplementation did not promote a significant reduction in bacterial numbers. Overall, there were no significant changes in the isolates’ virulence or resistance profiles, and none of the isolates obtained were classified as high or moderate threats based on their MAR and VIR indexes. The most pathogenic <i>Enterococcus</i> isolates found were present in the control samples. A phylogenetic analysis of <i>E. coli</i> isolates allowed us to identify phylogroup D strains, predominantly in RMS supplemented with 2.5% and 10% biochar, which are associated with higher virulence and resistance. These findings indicate that the biochar concentrations tested were not effective in significantly reducing the bacterial risks associated with RMS. Further research is needed to evaluate different biochar formulations and concentrations, aiming to optimize its potential use for RMS supplementation.https://www.mdpi.com/2306-7381/12/1/43dairy farmsrecycled manure solids (RMS)biocharantimicrobial resistance (AMR)virulence factors<i>Escherichia coli</i> |
| spellingShingle | Ana José Pires Ana Filipa Esteves Gonçalo Pereira Catarina Geraldes Joana Fernandes Guerreiro Raquel Abreu Rita Magalhães Lélia Chambel Elisabete Silva David Fangueiro Manuela Oliveira Ricardo Bexiga Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy Cows Veterinary Sciences dairy farms recycled manure solids (RMS) biochar antimicrobial resistance (AMR) virulence factors <i>Escherichia coli</i> |
| title | Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy Cows |
| title_full | Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy Cows |
| title_fullStr | Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy Cows |
| title_full_unstemmed | Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy Cows |
| title_short | Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy Cows |
| title_sort | potential of pine biochar to mitigate bacterial hazards present in recycled manure solids from dairy cows |
| topic | dairy farms recycled manure solids (RMS) biochar antimicrobial resistance (AMR) virulence factors <i>Escherichia coli</i> |
| url | https://www.mdpi.com/2306-7381/12/1/43 |
| work_keys_str_mv | AT anajosepires potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT anafilipaesteves potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT goncalopereira potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT catarinageraldes potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT joanafernandesguerreiro potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT raquelabreu potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT ritamagalhaes potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT leliachambel potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT elisabetesilva potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT davidfangueiro potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT manuelaoliveira potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows AT ricardobexiga potentialofpinebiochartomitigatebacterialhazardspresentinrecycledmanuresolidsfromdairycows |