Compound K Promotes Megakaryocytic Differentiation by NLRP3 Inflammasome Activation
Platelets are essential blood components that maintain hemostasis, prevent excessive bleeding, and facilitate wound healing. Reduced platelet counts are implicated in various diseases, including leukemia, hepatitis, cancer, and Alzheimer’s disease. Enhancing megakaryocytic differentiation is a promi...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/14/10/1257 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850204962918760448 |
|---|---|
| author | Seonhwa Hwang Min-Seo Park Anthony Junhoe Koo Eunsoo Yoo Seh-Hyon Song Hye-Kyung Kim Min-Hi Park Jae-Seon Kang |
| author_facet | Seonhwa Hwang Min-Seo Park Anthony Junhoe Koo Eunsoo Yoo Seh-Hyon Song Hye-Kyung Kim Min-Hi Park Jae-Seon Kang |
| author_sort | Seonhwa Hwang |
| collection | DOAJ |
| description | Platelets are essential blood components that maintain hemostasis, prevent excessive bleeding, and facilitate wound healing. Reduced platelet counts are implicated in various diseases, including leukemia, hepatitis, cancer, and Alzheimer’s disease. Enhancing megakaryocytic differentiation is a promising strategy to increase platelet production. Compound K (CK), a major bioactive metabolite of ginsenosides from <i>Panax ginseng</i>, has demonstrated anti-cancer and neuroprotective properties. In this study, we investigated the effects of CK on megakaryocytic differentiation and apoptosis in chronic myeloid leukemia (CML) cell lines K562 and Meg-01. CK treatment significantly upregulated the mRNA expression of key megakaryocytic differentiation markers, including CD61, CD41, and CD42a, and promoted the formation of large, multinucleated cells in K562 cells. Additionally, flow cytometry analysis revealed that CK at 5 µM induced apoptosis, a critical process in thrombocytopoiesis, in both K562 and Meg-01 cells. RT<sup>2</sup> Profiler PCR array analysis further identified a marked increase in the expression of genes associated with the activation of the NLRP3 inflammasome in CK-treated K562 and Meg-01 cells. This study is the first to demonstrate that CK promotes megakaryocytic differentiation and apoptosis through the activation of the ERK/EGR1 and NLRP3 inflammasome pathways. These findings suggest that CK may enhance platelet production, indicating its potential as a therapeutic candidate for platelet-related disorders and other associated diseases. |
| format | Article |
| id | doaj-art-5dc79d4015ab4f1dbc1fe77378f6efe3 |
| institution | OA Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-5dc79d4015ab4f1dbc1fe77378f6efe32025-08-20T02:11:12ZengMDPI AGBiomolecules2218-273X2024-10-011410125710.3390/biom14101257Compound K Promotes Megakaryocytic Differentiation by NLRP3 Inflammasome ActivationSeonhwa Hwang0Min-Seo Park1Anthony Junhoe Koo2Eunsoo Yoo3Seh-Hyon Song4Hye-Kyung Kim5Min-Hi Park6Jae-Seon Kang7College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of KoreaCollege of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of KoreaCollege of Engineering, North Carolina A&T State University, Greensboro, NC 27411, USAChemical, Biological, and Bioengineering Department, North Carolina A&T State University, Greensboro, NC 27411, USACollege of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of KoreaCollege of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of KoreaCollege of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of KoreaCollege of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of KoreaPlatelets are essential blood components that maintain hemostasis, prevent excessive bleeding, and facilitate wound healing. Reduced platelet counts are implicated in various diseases, including leukemia, hepatitis, cancer, and Alzheimer’s disease. Enhancing megakaryocytic differentiation is a promising strategy to increase platelet production. Compound K (CK), a major bioactive metabolite of ginsenosides from <i>Panax ginseng</i>, has demonstrated anti-cancer and neuroprotective properties. In this study, we investigated the effects of CK on megakaryocytic differentiation and apoptosis in chronic myeloid leukemia (CML) cell lines K562 and Meg-01. CK treatment significantly upregulated the mRNA expression of key megakaryocytic differentiation markers, including CD61, CD41, and CD42a, and promoted the formation of large, multinucleated cells in K562 cells. Additionally, flow cytometry analysis revealed that CK at 5 µM induced apoptosis, a critical process in thrombocytopoiesis, in both K562 and Meg-01 cells. RT<sup>2</sup> Profiler PCR array analysis further identified a marked increase in the expression of genes associated with the activation of the NLRP3 inflammasome in CK-treated K562 and Meg-01 cells. This study is the first to demonstrate that CK promotes megakaryocytic differentiation and apoptosis through the activation of the ERK/EGR1 and NLRP3 inflammasome pathways. These findings suggest that CK may enhance platelet production, indicating its potential as a therapeutic candidate for platelet-related disorders and other associated diseases.https://www.mdpi.com/2218-273X/14/10/1257compound Kmegakaryocytic differentiationleukemia cellNLRP3 inflammasomeplatelets |
| spellingShingle | Seonhwa Hwang Min-Seo Park Anthony Junhoe Koo Eunsoo Yoo Seh-Hyon Song Hye-Kyung Kim Min-Hi Park Jae-Seon Kang Compound K Promotes Megakaryocytic Differentiation by NLRP3 Inflammasome Activation Biomolecules compound K megakaryocytic differentiation leukemia cell NLRP3 inflammasome platelets |
| title | Compound K Promotes Megakaryocytic Differentiation by NLRP3 Inflammasome Activation |
| title_full | Compound K Promotes Megakaryocytic Differentiation by NLRP3 Inflammasome Activation |
| title_fullStr | Compound K Promotes Megakaryocytic Differentiation by NLRP3 Inflammasome Activation |
| title_full_unstemmed | Compound K Promotes Megakaryocytic Differentiation by NLRP3 Inflammasome Activation |
| title_short | Compound K Promotes Megakaryocytic Differentiation by NLRP3 Inflammasome Activation |
| title_sort | compound k promotes megakaryocytic differentiation by nlrp3 inflammasome activation |
| topic | compound K megakaryocytic differentiation leukemia cell NLRP3 inflammasome platelets |
| url | https://www.mdpi.com/2218-273X/14/10/1257 |
| work_keys_str_mv | AT seonhwahwang compoundkpromotesmegakaryocyticdifferentiationbynlrp3inflammasomeactivation AT minseopark compoundkpromotesmegakaryocyticdifferentiationbynlrp3inflammasomeactivation AT anthonyjunhoekoo compoundkpromotesmegakaryocyticdifferentiationbynlrp3inflammasomeactivation AT eunsooyoo compoundkpromotesmegakaryocyticdifferentiationbynlrp3inflammasomeactivation AT sehhyonsong compoundkpromotesmegakaryocyticdifferentiationbynlrp3inflammasomeactivation AT hyekyungkim compoundkpromotesmegakaryocyticdifferentiationbynlrp3inflammasomeactivation AT minhipark compoundkpromotesmegakaryocyticdifferentiationbynlrp3inflammasomeactivation AT jaeseonkang compoundkpromotesmegakaryocyticdifferentiationbynlrp3inflammasomeactivation |