Control of tissue flows and embryo geometry in avian gastrulation

Abstract Embryonic tissues undergo coordinated flows during avian gastrulation to establish the body plan. Here, we elucidate how the interplay between embryonic and extraembryonic tissues affects the chick embryo’s size and shape. These two distinct geometric changes are each associated with dynami...

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Main Authors: Guillermo Serrano Nájera, Alex M. Plum, Ben Steventon, Cornelis J. Weijer, Mattia Serra
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-60249-8
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author Guillermo Serrano Nájera
Alex M. Plum
Ben Steventon
Cornelis J. Weijer
Mattia Serra
author_facet Guillermo Serrano Nájera
Alex M. Plum
Ben Steventon
Cornelis J. Weijer
Mattia Serra
author_sort Guillermo Serrano Nájera
collection DOAJ
description Abstract Embryonic tissues undergo coordinated flows during avian gastrulation to establish the body plan. Here, we elucidate how the interplay between embryonic and extraembryonic tissues affects the chick embryo’s size and shape. These two distinct geometric changes are each associated with dynamic curves across which trajectories separate (kinematic repellers). Through physical modeling and experimental manipulations of both embryonic and extraembryonic tissues, we selectively eliminate either or both repellers in model and experiments, revealing their mechanistic origins. We find that embryo size is affected by the competition between extraembryonic epiboly and embryonic myosin-driven contraction—which persists when mesoderm induction is blocked. Instead, the characteristic shape change from circular to pear-shaped arises from myosin-driven cell intercalations in the mesendoderm, irrespective of epiboly. These findings elucidate modular mechanisms controlling avian gastrulation flows and provide a mechanistic basis for the independent control of embryo size and shape during development.
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spelling doaj-art-5daa8b9f64764e8f9dc72cffab3a1f632025-08-20T03:10:34ZengNature PortfolioNature Communications2041-17232025-06-0116111110.1038/s41467-025-60249-8Control of tissue flows and embryo geometry in avian gastrulationGuillermo Serrano Nájera0Alex M. Plum1Ben Steventon2Cornelis J. Weijer3Mattia Serra4Department of Genetics, University of CambridgeDepartment of Physics, University of CaliforniaDepartment of Genetics, University of CambridgeDivision of Molec. Cell and Dev. Biology, School of Life Sciences, Univ. of DundeeDepartment of Physics, University of CaliforniaAbstract Embryonic tissues undergo coordinated flows during avian gastrulation to establish the body plan. Here, we elucidate how the interplay between embryonic and extraembryonic tissues affects the chick embryo’s size and shape. These two distinct geometric changes are each associated with dynamic curves across which trajectories separate (kinematic repellers). Through physical modeling and experimental manipulations of both embryonic and extraembryonic tissues, we selectively eliminate either or both repellers in model and experiments, revealing their mechanistic origins. We find that embryo size is affected by the competition between extraembryonic epiboly and embryonic myosin-driven contraction—which persists when mesoderm induction is blocked. Instead, the characteristic shape change from circular to pear-shaped arises from myosin-driven cell intercalations in the mesendoderm, irrespective of epiboly. These findings elucidate modular mechanisms controlling avian gastrulation flows and provide a mechanistic basis for the independent control of embryo size and shape during development.https://doi.org/10.1038/s41467-025-60249-8
spellingShingle Guillermo Serrano Nájera
Alex M. Plum
Ben Steventon
Cornelis J. Weijer
Mattia Serra
Control of tissue flows and embryo geometry in avian gastrulation
Nature Communications
title Control of tissue flows and embryo geometry in avian gastrulation
title_full Control of tissue flows and embryo geometry in avian gastrulation
title_fullStr Control of tissue flows and embryo geometry in avian gastrulation
title_full_unstemmed Control of tissue flows and embryo geometry in avian gastrulation
title_short Control of tissue flows and embryo geometry in avian gastrulation
title_sort control of tissue flows and embryo geometry in avian gastrulation
url https://doi.org/10.1038/s41467-025-60249-8
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