Multiplex PCR targeting IS6110 and mpb64 Genes for Detecting Extra- Pulmonary Tuberculosis: A Cross-Sectional Study in a Tertiary Care Center, Kashmir, India
Introduction: Extra-pulmonary tuberculosis (EPTB) is a significant cause of morbidity, and early diagnosis is critical for improving patient outcomes. Conventional diagnostic methods for EPTB often require improvement, highlighting the need for more rapid and sensitive diagnostic procedures. In t...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2023-06-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | https://jommid.pasteur.ac.ir/article-1-498-en.html |
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| Summary: | Introduction: Extra-pulmonary tuberculosis (EPTB) is a significant cause of
morbidity, and early diagnosis is critical for improving patient outcomes.
Conventional diagnostic methods for EPTB often require improvement,
highlighting the need for more rapid and sensitive diagnostic procedures. In this
cross-sectional study, we aimed to evaluate the diagnostic usefulness of
multiplex PCR (mPCR) using IS6110 and mpb64 as gene targets for detecting
Mycobacterium tuberculosis in samples from suspected cases of EPTB. We
compared the performance of mPCR with conventional methods, including
Ziehl Neelsen (ZN) microscopy, culture in LJ media, and BacT/Alert system.
Our study aimed to provide insight into the utility of mPCR and its different
targets for diagnosing EPTB in our setting. Methods: We conducted a crosssectional
survey of 250 non-repeat clinical samples from extrapulmonary sites
to detect M. tuberculosis. Both conventional diagnostic methods, including ZN
microscopy, culture in LJ media, and BacT/Alert system, and molecular
methods, including multiplex PCR (mPCR) using IS6110 and mpb64 as gene
targets, were performed on the samples. Of the 250 samples, results for all the
diagnostic methods were available for 116 samples, which were included in the
final analysis. The study population comprised 83 patients with suspected EPTB
and 33 controls. Results: Among the 83 samples in the EPTB group,
conventional diagnostic methods, including ZN microscopy, LJ culture, and
BacT/Alert system, showed low positivity rates of 6.02%, 8.43%, and 15.66%,
respectively. In contrast, multiplex PCR (mPCR) using IS6110 and mpb64 as
gene targets showed a significantly higher positivity rate of 79.51%. The IS6110
gene was amplified in 79.51% of the samples, while mpb64 was amplified in
49.39%. Conclusion: Our study demonstrates that multiplex PCR (mPCR)
using IS6110 and mpb64 as gene targets is a more sensitive diagnostic method
for extra-pulmonary tuberculosis (EPTB) than conventional methods. Both
IS6110 and mpb64 showed high sensitivity of 100%, but mpb64 was more
specific when compared with the gold standard. Our findings suggest that
mPCR, particularly with the inclusion of mpb64 as the target gene, may be a
valuable tool for the early and accurate diagnosis of EPTB. |
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| ISSN: | 2345-5349 2345-5330 |