Tailor-made fermentation of sprouted wheat and barley flours and their application in bread making: A comprehensive comparison with conventional approaches in the baking industry

This study investigates the development and application of type III sourdoughs, produced by fermenting sprouted wheat and barley flours with carefully selected lactic acid bacteria (LAB). Two optimized combinations of LAB strains were used: Furfurilactobacillus rossiae (CR5), Weissella confusa T6B10...

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Main Authors: Giuseppe Perri, Graziana Difonzo, Lorenzo Ciraldo, Federico Rametta, Gaia Gadaleta-Caldarola, Hana Ameur, Olga Nikoloudaki, Maria De Angelis, Francesco Caponio, Erica Pontonio
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Current Research in Food Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S266592712500084X
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author Giuseppe Perri
Graziana Difonzo
Lorenzo Ciraldo
Federico Rametta
Gaia Gadaleta-Caldarola
Hana Ameur
Olga Nikoloudaki
Maria De Angelis
Francesco Caponio
Erica Pontonio
author_facet Giuseppe Perri
Graziana Difonzo
Lorenzo Ciraldo
Federico Rametta
Gaia Gadaleta-Caldarola
Hana Ameur
Olga Nikoloudaki
Maria De Angelis
Francesco Caponio
Erica Pontonio
author_sort Giuseppe Perri
collection DOAJ
description This study investigates the development and application of type III sourdoughs, produced by fermenting sprouted wheat and barley flours with carefully selected lactic acid bacteria (LAB). Two optimized combinations of LAB strains were used: Furfurilactobacillus rossiae (CR5), Weissella confusa T6B10, and Lactiplantibacillus plantarum SB88.B4 for sprouted wheat flour; and Leuconostoc pseudomesenteroides DSM 20193, L. plantarum 7A, and F. rossiae (CR5) for sprouted barley flours. Fermentation resulted in substantial increases in peptide content (450 % in sprouted wheat flour-based sourdough and 520 % in sprouted barley flour-based sourdough) and phenolic compounds (344 % and 261 %, respectively), along with improved antioxidant activity (100 % in wheat and 40 % in barley). Among the experimental breads, those made with sprouted barley sourdough demonstrated the highest nutritional and functional benefits, including a highest content of dietary fiber, improved in vitro protein digestibility (IVPD, 81.14 %), a reduced predicted glycemic index (pGI, 84.78 %), and strong angiotensin-converting enzyme (ACE) inhibitory activity (73 %). The rheological behaviour of doughs incorporating novel type III sourdoughs was comparable to those containing type II wheat sourdoughs combined with enzymatyc enanchers, indicating their suitability for baking applications. Sensory evaluations highlighted that bread made with type III sourdough from sprouted wheat flour was appreciated for its enhanced crust and crumb colour, while bread made with sprouted barley sourdough stood out for its rich bran aroma, toasted notes, and balanced acidity. This study highlights the potential of targeted fermentation of sprouted flours as a key solution to address the growing demand for health-focused and eco-friendly innovations from both consumers and producers.
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spelling doaj-art-5da94efe215a408f8aa444135c23911d2025-08-20T02:06:24ZengElsevierCurrent Research in Food Science2665-92712025-01-011010105310.1016/j.crfs.2025.101053Tailor-made fermentation of sprouted wheat and barley flours and their application in bread making: A comprehensive comparison with conventional approaches in the baking industryGiuseppe Perri0Graziana Difonzo1Lorenzo Ciraldo2Federico Rametta3Gaia Gadaleta-Caldarola4Hana Ameur5Olga Nikoloudaki6Maria De Angelis7Francesco Caponio8Erica Pontonio9Department of the Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, Via Amendola, 165/a, Bari, I-70126, Italy; Corresponding author.Department of the Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, Via Amendola, 165/a, Bari, I-70126, ItalyDepartment of the Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, Via Amendola, 165/a, Bari, I-70126, ItalyDepartment of the Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, Via Amendola, 165/a, Bari, I-70126, ItalyDepartment of the Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, Via Amendola, 165/a, Bari, I-70126, ItalyFaculty of Agricultural, Environmental and Food Sciences, Libera Universitá di Bolzano, Piazza Universitá, 5, 39100, Bolzano, ItalyFaculty of Agricultural, Environmental and Food Sciences, Libera Universitá di Bolzano, Piazza Universitá, 5, 39100, Bolzano, ItalyDepartment of the Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, Via Amendola, 165/a, Bari, I-70126, ItalyDepartment of the Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, Via Amendola, 165/a, Bari, I-70126, ItalyDepartment of the Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, Via Amendola, 165/a, Bari, I-70126, ItalyThis study investigates the development and application of type III sourdoughs, produced by fermenting sprouted wheat and barley flours with carefully selected lactic acid bacteria (LAB). Two optimized combinations of LAB strains were used: Furfurilactobacillus rossiae (CR5), Weissella confusa T6B10, and Lactiplantibacillus plantarum SB88.B4 for sprouted wheat flour; and Leuconostoc pseudomesenteroides DSM 20193, L. plantarum 7A, and F. rossiae (CR5) for sprouted barley flours. Fermentation resulted in substantial increases in peptide content (450 % in sprouted wheat flour-based sourdough and 520 % in sprouted barley flour-based sourdough) and phenolic compounds (344 % and 261 %, respectively), along with improved antioxidant activity (100 % in wheat and 40 % in barley). Among the experimental breads, those made with sprouted barley sourdough demonstrated the highest nutritional and functional benefits, including a highest content of dietary fiber, improved in vitro protein digestibility (IVPD, 81.14 %), a reduced predicted glycemic index (pGI, 84.78 %), and strong angiotensin-converting enzyme (ACE) inhibitory activity (73 %). The rheological behaviour of doughs incorporating novel type III sourdoughs was comparable to those containing type II wheat sourdoughs combined with enzymatyc enanchers, indicating their suitability for baking applications. Sensory evaluations highlighted that bread made with type III sourdough from sprouted wheat flour was appreciated for its enhanced crust and crumb colour, while bread made with sprouted barley sourdough stood out for its rich bran aroma, toasted notes, and balanced acidity. This study highlights the potential of targeted fermentation of sprouted flours as a key solution to address the growing demand for health-focused and eco-friendly innovations from both consumers and producers.http://www.sciencedirect.com/science/article/pii/S266592712500084XLactic acid bacteriaSprouted floursBioactive compoundsBaking industryTailor made foodWhole grains
spellingShingle Giuseppe Perri
Graziana Difonzo
Lorenzo Ciraldo
Federico Rametta
Gaia Gadaleta-Caldarola
Hana Ameur
Olga Nikoloudaki
Maria De Angelis
Francesco Caponio
Erica Pontonio
Tailor-made fermentation of sprouted wheat and barley flours and their application in bread making: A comprehensive comparison with conventional approaches in the baking industry
Current Research in Food Science
Lactic acid bacteria
Sprouted flours
Bioactive compounds
Baking industry
Tailor made food
Whole grains
title Tailor-made fermentation of sprouted wheat and barley flours and their application in bread making: A comprehensive comparison with conventional approaches in the baking industry
title_full Tailor-made fermentation of sprouted wheat and barley flours and their application in bread making: A comprehensive comparison with conventional approaches in the baking industry
title_fullStr Tailor-made fermentation of sprouted wheat and barley flours and their application in bread making: A comprehensive comparison with conventional approaches in the baking industry
title_full_unstemmed Tailor-made fermentation of sprouted wheat and barley flours and their application in bread making: A comprehensive comparison with conventional approaches in the baking industry
title_short Tailor-made fermentation of sprouted wheat and barley flours and their application in bread making: A comprehensive comparison with conventional approaches in the baking industry
title_sort tailor made fermentation of sprouted wheat and barley flours and their application in bread making a comprehensive comparison with conventional approaches in the baking industry
topic Lactic acid bacteria
Sprouted flours
Bioactive compounds
Baking industry
Tailor made food
Whole grains
url http://www.sciencedirect.com/science/article/pii/S266592712500084X
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