Rhamnose polysaccharide-decorated outer membrane vesicles as a vaccine candidate targeting Group A Streptococcus from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis
Group A Streptococcus (Strep A) cause a wide range of human-exclusive infections, annually killing more than 500,000 people. Antibiotic resistance incidence of invasive Strep A tripled in the past decade and emphasises the need to develop a universal Strep A vaccine. In this study, we developed reco...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Vaccine: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590136225000701 |
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| author | Sowmya Ajay Castro Sarah Thomson Helen Alexandra Shaw Azul Zorzoli Benjamin H. Meyer Mark Reglinski Mark McNeil Helge C. Dorfmueller |
| author_facet | Sowmya Ajay Castro Sarah Thomson Helen Alexandra Shaw Azul Zorzoli Benjamin H. Meyer Mark Reglinski Mark McNeil Helge C. Dorfmueller |
| author_sort | Sowmya Ajay Castro |
| collection | DOAJ |
| description | Group A Streptococcus (Strep A) cause a wide range of human-exclusive infections, annually killing more than 500,000 people. Antibiotic resistance incidence of invasive Strep A tripled in the past decade and emphasises the need to develop a universal Strep A vaccine. In this study, we developed recombinant rhamnose polysaccharides (RhaPS), a validated universal Strep A vaccine candidate, presented on E. coli outer membrane vesicles (OMVs). We investigated OMV-RhaPS for their immunogenicity in the mouse and rabbit models. Through flow cytometry, ELISA, and immunofluorescence microscopy, we demonstrated that RhaPS-specific antibodies recognise Strep A strains via the Group A Carbohydrate (GAC) in S. pyogenes and the newly emerged S. dysgalactiae subsp. equisimilis. Elevated IL-17A levels from RhaPS-OMV-immunised splenocytes were detected when re-stimulated with the immunogen RhaPS-OMV. We report the efficacy and potency of recombinant produced RhaPS triggering antibodies that recognise Strep A bacteria and facilitate monocyte dependent opsonophagocytosis of several Strep A serotypes. |
| format | Article |
| id | doaj-art-5da16d9d57f04d7b9503ee1932e33b80 |
| institution | Kabale University |
| issn | 2590-1362 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Vaccine: X |
| spelling | doaj-art-5da16d9d57f04d7b9503ee1932e33b802025-08-20T03:36:35ZengElsevierVaccine: X2590-13622025-08-012510067610.1016/j.jvacx.2025.100676Rhamnose polysaccharide-decorated outer membrane vesicles as a vaccine candidate targeting Group A Streptococcus from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilisSowmya Ajay Castro0Sarah Thomson1Helen Alexandra Shaw2Azul Zorzoli3Benjamin H. Meyer4Mark Reglinski5Mark McNeil6Helge C. Dorfmueller7Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, United KingdomBiological Services, School of Life Sciences, University of Dundee, Dundee, United KingdomVaccine Division, Scientific Research & Innovation Group, MHRA, Potters Bar, United KingdomMolecular Microbiology, School of Life Sciences, University of Dundee, Dundee, United KingdomMolecular Microbiology, School of Life Sciences, University of Dundee, Dundee, United KingdomMolecular Microbiology, School of Life Sciences, University of Dundee, Dundee, United KingdomMolecular Microbiology, School of Life Sciences, University of Dundee, Dundee, United KingdomMolecular Microbiology, School of Life Sciences, University of Dundee, Dundee, United Kingdom; Corresponding author.Group A Streptococcus (Strep A) cause a wide range of human-exclusive infections, annually killing more than 500,000 people. Antibiotic resistance incidence of invasive Strep A tripled in the past decade and emphasises the need to develop a universal Strep A vaccine. In this study, we developed recombinant rhamnose polysaccharides (RhaPS), a validated universal Strep A vaccine candidate, presented on E. coli outer membrane vesicles (OMVs). We investigated OMV-RhaPS for their immunogenicity in the mouse and rabbit models. Through flow cytometry, ELISA, and immunofluorescence microscopy, we demonstrated that RhaPS-specific antibodies recognise Strep A strains via the Group A Carbohydrate (GAC) in S. pyogenes and the newly emerged S. dysgalactiae subsp. equisimilis. Elevated IL-17A levels from RhaPS-OMV-immunised splenocytes were detected when re-stimulated with the immunogen RhaPS-OMV. We report the efficacy and potency of recombinant produced RhaPS triggering antibodies that recognise Strep A bacteria and facilitate monocyte dependent opsonophagocytosis of several Strep A serotypes.http://www.sciencedirect.com/science/article/pii/S2590136225000701Streptococcus pyogenesGroup a StreptococcusGroup a carbohydrateRhamnoseOuter membrane vesiclesM protein |
| spellingShingle | Sowmya Ajay Castro Sarah Thomson Helen Alexandra Shaw Azul Zorzoli Benjamin H. Meyer Mark Reglinski Mark McNeil Helge C. Dorfmueller Rhamnose polysaccharide-decorated outer membrane vesicles as a vaccine candidate targeting Group A Streptococcus from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis Vaccine: X Streptococcus pyogenes Group a Streptococcus Group a carbohydrate Rhamnose Outer membrane vesicles M protein |
| title | Rhamnose polysaccharide-decorated outer membrane vesicles as a vaccine candidate targeting Group A Streptococcus from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis |
| title_full | Rhamnose polysaccharide-decorated outer membrane vesicles as a vaccine candidate targeting Group A Streptococcus from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis |
| title_fullStr | Rhamnose polysaccharide-decorated outer membrane vesicles as a vaccine candidate targeting Group A Streptococcus from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis |
| title_full_unstemmed | Rhamnose polysaccharide-decorated outer membrane vesicles as a vaccine candidate targeting Group A Streptococcus from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis |
| title_short | Rhamnose polysaccharide-decorated outer membrane vesicles as a vaccine candidate targeting Group A Streptococcus from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis |
| title_sort | rhamnose polysaccharide decorated outer membrane vesicles as a vaccine candidate targeting group a streptococcus from streptococcus pyogenes and streptococcus dysgalactiae subsp equisimilis |
| topic | Streptococcus pyogenes Group a Streptococcus Group a carbohydrate Rhamnose Outer membrane vesicles M protein |
| url | http://www.sciencedirect.com/science/article/pii/S2590136225000701 |
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