Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway
Free cholesterol has been considered to be a critical risk factor of nonalcoholic fatty liver disease (NAFLD). It remains unknown whether dietary intake of condensed tannins (CTs) have distinguishable effects to alleviate liver damage caused by a high cholesterol diet. Male C57BL/6 mice were fed a h...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-03-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250078 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850223164980723712 |
|---|---|
| author | Xiangxin Li Yijing Pu Bangdi Liu Xiaoming Fang Wenjun Peng Weibo Jiang Wenli Tian |
| author_facet | Xiangxin Li Yijing Pu Bangdi Liu Xiaoming Fang Wenjun Peng Weibo Jiang Wenli Tian |
| author_sort | Xiangxin Li |
| collection | DOAJ |
| description | Free cholesterol has been considered to be a critical risk factor of nonalcoholic fatty liver disease (NAFLD). It remains unknown whether dietary intake of condensed tannins (CTs) have distinguishable effects to alleviate liver damage caused by a high cholesterol diet. Male C57BL/6 mice were fed a high cholesterol diet for 6 weeks, and given CTs treatment at a dosage of 200 mg/(kg·day) at the same time. The results indicated that compared with mice fed a normal diet, a high cholesterol diet group resulted in significant weight loss, dysregulation of lipid metabolism in blood and liver, and oxidative stress in the liver, but CTs treatment dramatically reversed these negative effects. Hematoxylin and eosin (H&E) staining and frozen section observation manifested that CTs treatment could effectively reduce the deposition of liver cholesterol and tissue necrosis caused by high cholesterol intake. CTs alleviated liver injury mainly by regulating the expression of related genes in cholesterol metabolism pathway and AMPK phosphorylation. Our results confirmed that CTs have remarkable cholesterol lowering and anti-liver injury effects in vivo. |
| format | Article |
| id | doaj-art-5d9ff854d013459f80dcc769ca7e82fe |
| institution | OA Journals |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-5d9ff854d013459f80dcc769ca7e82fe2025-08-20T02:06:04ZengTsinghua University PressFood Science and Human Wellness2213-45302024-03-0113290991910.26599/FSHW.2022.9250078Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathwayXiangxin Li0Yijing Pu1Bangdi Liu2Xiaoming Fang3Wenjun Peng4Weibo Jiang5Wenli Tian6Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaAcademy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaFree cholesterol has been considered to be a critical risk factor of nonalcoholic fatty liver disease (NAFLD). It remains unknown whether dietary intake of condensed tannins (CTs) have distinguishable effects to alleviate liver damage caused by a high cholesterol diet. Male C57BL/6 mice were fed a high cholesterol diet for 6 weeks, and given CTs treatment at a dosage of 200 mg/(kg·day) at the same time. The results indicated that compared with mice fed a normal diet, a high cholesterol diet group resulted in significant weight loss, dysregulation of lipid metabolism in blood and liver, and oxidative stress in the liver, but CTs treatment dramatically reversed these negative effects. Hematoxylin and eosin (H&E) staining and frozen section observation manifested that CTs treatment could effectively reduce the deposition of liver cholesterol and tissue necrosis caused by high cholesterol intake. CTs alleviated liver injury mainly by regulating the expression of related genes in cholesterol metabolism pathway and AMPK phosphorylation. Our results confirmed that CTs have remarkable cholesterol lowering and anti-liver injury effects in vivo.https://www.sciopen.com/article/10.26599/FSHW.2022.9250078condensed tanninshigh cholesterolliver injuryantioxidantstranscriptomic analysis |
| spellingShingle | Xiangxin Li Yijing Pu Bangdi Liu Xiaoming Fang Wenjun Peng Weibo Jiang Wenli Tian Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway Food Science and Human Wellness condensed tannins high cholesterol liver injury antioxidants transcriptomic analysis |
| title | Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway |
| title_full | Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway |
| title_fullStr | Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway |
| title_full_unstemmed | Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway |
| title_short | Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway |
| title_sort | biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway |
| topic | condensed tannins high cholesterol liver injury antioxidants transcriptomic analysis |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250078 |
| work_keys_str_mv | AT xiangxinli biochemistryandtranscriptomeanalysisrevealcondensedtanninsalleviateliverinjuryinducedbyregulatingcholesterolmetabolismpathway AT yijingpu biochemistryandtranscriptomeanalysisrevealcondensedtanninsalleviateliverinjuryinducedbyregulatingcholesterolmetabolismpathway AT bangdiliu biochemistryandtranscriptomeanalysisrevealcondensedtanninsalleviateliverinjuryinducedbyregulatingcholesterolmetabolismpathway AT xiaomingfang biochemistryandtranscriptomeanalysisrevealcondensedtanninsalleviateliverinjuryinducedbyregulatingcholesterolmetabolismpathway AT wenjunpeng biochemistryandtranscriptomeanalysisrevealcondensedtanninsalleviateliverinjuryinducedbyregulatingcholesterolmetabolismpathway AT weibojiang biochemistryandtranscriptomeanalysisrevealcondensedtanninsalleviateliverinjuryinducedbyregulatingcholesterolmetabolismpathway AT wenlitian biochemistryandtranscriptomeanalysisrevealcondensedtanninsalleviateliverinjuryinducedbyregulatingcholesterolmetabolismpathway |