Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery
Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach. In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coa...
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| Format: | Article |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1658594 |
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| author | Mohammad Porgham Daryasari Mehdi Dusti Telgerd Mohammad Hossein Karami Ali Zandi-Karimi Hamid Akbarijavar Mehdi Khoobi Ehsan Seyedjafari Gebremariam Birhanu Pegah Khosravian Fatemeh SadatMahdavi |
| author_facet | Mohammad Porgham Daryasari Mehdi Dusti Telgerd Mohammad Hossein Karami Ali Zandi-Karimi Hamid Akbarijavar Mehdi Khoobi Ehsan Seyedjafari Gebremariam Birhanu Pegah Khosravian Fatemeh SadatMahdavi |
| author_sort | Mohammad Porgham Daryasari |
| collection | DOAJ |
| description | Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach. In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application. The MSN was prepared by precipitation method. After that, Dexamethasone (Dexa) was loaded into MSNs (MSN-Dexa). In the following, CS was coated over the prepared nanoparticles to form MSN-Dexa@CS and then, were mixed to PLLA solution to form MSN-Dexa@CS/PLLA composite for electrospinning. The surface morphology, hydrophilicity, tensile strength and the bioactivity of the scaffolds were characterized. The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition. The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds. Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration. |
| format | Article |
| id | doaj-art-5d9e42bece614aa28e2acf4348dd3bc0 |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-5d9e42bece614aa28e2acf4348dd3bc02025-08-20T03:51:08ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014714020402910.1080/21691401.2019.1658594Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone deliveryMohammad Porgham Daryasari0Mehdi Dusti Telgerd1Mohammad Hossein Karami2Ali Zandi-Karimi3Hamid Akbarijavar4Mehdi Khoobi5Ehsan Seyedjafari6Gebremariam Birhanu7Pegah Khosravian8Fatemeh SadatMahdavi9Department of Pharmaceutical Biomaterials, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranDepartment of Pharmaceutical Biomaterials, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranDepartment of Biotechnology, College of Science, University of Tehran, Tehran, IranDepartment of Biotechnology, College of Science, University of Tehran, Tehran, IranDepartment of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranBiomaterials Group, the Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, IranDepartment of Biotechnology, College of Science, University of Tehran, Tehran, IranDepartment of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, International campus (TUMS-IC), Tehran, IranMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranDepartment of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, IranNowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach. In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application. The MSN was prepared by precipitation method. After that, Dexamethasone (Dexa) was loaded into MSNs (MSN-Dexa). In the following, CS was coated over the prepared nanoparticles to form MSN-Dexa@CS and then, were mixed to PLLA solution to form MSN-Dexa@CS/PLLA composite for electrospinning. The surface morphology, hydrophilicity, tensile strength and the bioactivity of the scaffolds were characterized. The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition. The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds. Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.https://www.tandfonline.com/doi/10.1080/21691401.2019.1658594Mesoporous silica nanoparticleselectrospinningPoly-l-lactic acidchitosandexamethasonecomposite scaffold |
| spellingShingle | Mohammad Porgham Daryasari Mehdi Dusti Telgerd Mohammad Hossein Karami Ali Zandi-Karimi Hamid Akbarijavar Mehdi Khoobi Ehsan Seyedjafari Gebremariam Birhanu Pegah Khosravian Fatemeh SadatMahdavi Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery Artificial Cells, Nanomedicine, and Biotechnology Mesoporous silica nanoparticles electrospinning Poly-l-lactic acid chitosan dexamethasone composite scaffold |
| title | Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery |
| title_full | Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery |
| title_fullStr | Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery |
| title_full_unstemmed | Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery |
| title_short | Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery |
| title_sort | poly l lactic acid scaffold incorporated chitosan coated mesoporous silica nanoparticles as ph sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery |
| topic | Mesoporous silica nanoparticles electrospinning Poly-l-lactic acid chitosan dexamethasone composite scaffold |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1658594 |
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