Effects of Different Modified Biochars on Growth of <i>Kosteletzkya virginica</i> and Corresponding Transcriptome Analysis
Modified biochar can effectively improve the quality and environment of coastal saline–alkali soil, but its effects on the growth and development of halophytes and its mechanism are still unclear. This study systematically evaluated the growth-promoting effects and preliminary mechanisms of H<sub...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/12/1849 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849704896835616768 |
|---|---|
| author | Hao Dai Mingyun Jia Jianhui Xue Yuying Huang Jinping Yu |
| author_facet | Hao Dai Mingyun Jia Jianhui Xue Yuying Huang Jinping Yu |
| author_sort | Hao Dai |
| collection | DOAJ |
| description | Modified biochar can effectively improve the quality and environment of coastal saline–alkali soil, but its effects on the growth and development of halophytes and its mechanism are still unclear. This study systematically evaluated the growth-promoting effects and preliminary mechanisms of H<sub>3</sub>PO<sub>4</sub>-modified biochar (HBC) and H<sub>3</sub>PO<sub>4</sub>–kaolinite–biochar composite (HBCK) on the economically important halophyte <i>Kosteletzkya virginica</i>. The results demonstrated that the application of HBC/HBCK significantly enhanced plant growth, resulting in increases of over 55% in plant height and greater than 100% in biomass relative to the control. Multidimensional mechanistic analysis revealed the following: (1) accumulation of nitrogen (N), phosphorus (P), and potassium (K) increased by at least 40%, significantly enhancing nutrient uptake; (2) increases in the activities of superoxide dismutase (SOD) and peroxidase (POD) by over 100% and 70%, respectively, markedly boosting antioxidant capacity and effectively alleviating oxidative stress; (3) molecular regulation via the activation of transcription factor networks (HSP, MYB, TCP, AP2/ERF, bZIP, and NLP) and modulation of key genes in ABA, BR, and JA signaling pathways (CYP707A, CYP90, and OPR2), establishing a multi-layered stress adaptation and growth promotion system. Beyond assessing the growth-promoting effects of modified biochars, this study provides novel insights into the regulatory transcription factor networks and phytohormone signaling pathways, offering theoretical foundations for the molecular design of biochars for saline–alkali soil remediation. |
| format | Article |
| id | doaj-art-5d95c8276bbf4c3585d6c51ca63f2951 |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-5d95c8276bbf4c3585d6c51ca63f29512025-08-20T03:16:36ZengMDPI AGPlants2223-77472025-06-011412184910.3390/plants14121849Effects of Different Modified Biochars on Growth of <i>Kosteletzkya virginica</i> and Corresponding Transcriptome AnalysisHao Dai0Mingyun Jia1Jianhui Xue2Yuying Huang3Jinping Yu4Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaCollege of Biomedicine and Health, Anhui Science and Technology University, Chuzhou 233100, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaModified biochar can effectively improve the quality and environment of coastal saline–alkali soil, but its effects on the growth and development of halophytes and its mechanism are still unclear. This study systematically evaluated the growth-promoting effects and preliminary mechanisms of H<sub>3</sub>PO<sub>4</sub>-modified biochar (HBC) and H<sub>3</sub>PO<sub>4</sub>–kaolinite–biochar composite (HBCK) on the economically important halophyte <i>Kosteletzkya virginica</i>. The results demonstrated that the application of HBC/HBCK significantly enhanced plant growth, resulting in increases of over 55% in plant height and greater than 100% in biomass relative to the control. Multidimensional mechanistic analysis revealed the following: (1) accumulation of nitrogen (N), phosphorus (P), and potassium (K) increased by at least 40%, significantly enhancing nutrient uptake; (2) increases in the activities of superoxide dismutase (SOD) and peroxidase (POD) by over 100% and 70%, respectively, markedly boosting antioxidant capacity and effectively alleviating oxidative stress; (3) molecular regulation via the activation of transcription factor networks (HSP, MYB, TCP, AP2/ERF, bZIP, and NLP) and modulation of key genes in ABA, BR, and JA signaling pathways (CYP707A, CYP90, and OPR2), establishing a multi-layered stress adaptation and growth promotion system. Beyond assessing the growth-promoting effects of modified biochars, this study provides novel insights into the regulatory transcription factor networks and phytohormone signaling pathways, offering theoretical foundations for the molecular design of biochars for saline–alkali soil remediation.https://www.mdpi.com/2223-7747/14/12/1849modified biochar<i>Kosteletzkya virginica</i>salt–alkali tolerancetranscription factor networkphytohormone signaling |
| spellingShingle | Hao Dai Mingyun Jia Jianhui Xue Yuying Huang Jinping Yu Effects of Different Modified Biochars on Growth of <i>Kosteletzkya virginica</i> and Corresponding Transcriptome Analysis Plants modified biochar <i>Kosteletzkya virginica</i> salt–alkali tolerance transcription factor network phytohormone signaling |
| title | Effects of Different Modified Biochars on Growth of <i>Kosteletzkya virginica</i> and Corresponding Transcriptome Analysis |
| title_full | Effects of Different Modified Biochars on Growth of <i>Kosteletzkya virginica</i> and Corresponding Transcriptome Analysis |
| title_fullStr | Effects of Different Modified Biochars on Growth of <i>Kosteletzkya virginica</i> and Corresponding Transcriptome Analysis |
| title_full_unstemmed | Effects of Different Modified Biochars on Growth of <i>Kosteletzkya virginica</i> and Corresponding Transcriptome Analysis |
| title_short | Effects of Different Modified Biochars on Growth of <i>Kosteletzkya virginica</i> and Corresponding Transcriptome Analysis |
| title_sort | effects of different modified biochars on growth of i kosteletzkya virginica i and corresponding transcriptome analysis |
| topic | modified biochar <i>Kosteletzkya virginica</i> salt–alkali tolerance transcription factor network phytohormone signaling |
| url | https://www.mdpi.com/2223-7747/14/12/1849 |
| work_keys_str_mv | AT haodai effectsofdifferentmodifiedbiocharsongrowthofikosteletzkyavirginicaiandcorrespondingtranscriptomeanalysis AT mingyunjia effectsofdifferentmodifiedbiocharsongrowthofikosteletzkyavirginicaiandcorrespondingtranscriptomeanalysis AT jianhuixue effectsofdifferentmodifiedbiocharsongrowthofikosteletzkyavirginicaiandcorrespondingtranscriptomeanalysis AT yuyinghuang effectsofdifferentmodifiedbiocharsongrowthofikosteletzkyavirginicaiandcorrespondingtranscriptomeanalysis AT jinpingyu effectsofdifferentmodifiedbiocharsongrowthofikosteletzkyavirginicaiandcorrespondingtranscriptomeanalysis |