The effect of visfatin on the transcriptomic profile of porcine anterior pituitary cells during periimplantation period
Abstract Females’ reproductive capacity is closely related to the actual metabolic status of the organism. The pituitary, an element of the regulatory hypothalamic-pituitary-ovarian axis, is one of the most important endocrine glands regulating reproductive system activity. Undisturbed functioning o...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-05-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-00766-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850277763380936704 |
|---|---|
| author | Kamil Dobrzyn Grzegorz Kopij Marta Kiezun Ewa Zaobidna Marlena Gudelska Barbara Zarzecka Katarzyna Kisielewska Agnieszka Rak Nina Smolinska Tadeusz Kaminski |
| author_facet | Kamil Dobrzyn Grzegorz Kopij Marta Kiezun Ewa Zaobidna Marlena Gudelska Barbara Zarzecka Katarzyna Kisielewska Agnieszka Rak Nina Smolinska Tadeusz Kaminski |
| author_sort | Kamil Dobrzyn |
| collection | DOAJ |
| description | Abstract Females’ reproductive capacity is closely related to the actual metabolic status of the organism. The pituitary, an element of the regulatory hypothalamic-pituitary-ovarian axis, is one of the most important endocrine glands regulating reproductive system activity. Undisturbed functioning of pituitary ensures the regular course of pregnancy through, among others, the modulation of steroid hormones production, which is critical in the early stages of gestation. Visfatin, a hormone belonging to the adipokines family, may belong to a group of factors regulating the reproductive functions in response to the female’s metabolic status. Herein we verified the hypothesis assuming a modulatory effect of visfatin on the porcine anterior pituitary transcriptome on days 15 to 16 of gestation (beginning of implantation). RNA-seq analysis of the porcine anterior pituitary cells revealed changes in the expression of 203 genes (121 up-regulated and 82 down-regulated, when compared to the non-treated controls), assigned to 325 gene ontology terms. The presence of visfatin affected the frequency of alternative splicing events (194 cases), as well as long noncoding RNA expression (64 cases). Visfatin expression and the occurrence of alternative splicing events of genes that are responsible, directly or indirectly, for regulation of the secretory functions of the pituitary, including those critical for reproductive functions suggests that the adipokine may be a key agent in ensuring the appropriate hormonal milieu during the peri-implantation period. |
| format | Article |
| id | doaj-art-5d958e2e950245d1ab7ec434309e526e |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-5d958e2e950245d1ab7ec434309e526e2025-08-20T01:49:44ZengNature PortfolioScientific Reports2045-23222025-05-0115111310.1038/s41598-025-00766-0The effect of visfatin on the transcriptomic profile of porcine anterior pituitary cells during periimplantation periodKamil Dobrzyn0Grzegorz Kopij1Marta Kiezun2Ewa Zaobidna3Marlena Gudelska4Barbara Zarzecka5Katarzyna Kisielewska6Agnieszka Rak7Nina Smolinska8Tadeusz Kaminski9Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Human Histology and Embryology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in OlsztynDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Human Histology and Embryology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in OlsztynLaboratory of Physiology and Toxicology of Reproduction, Institute of Zoology and Biomedical Research, Jagiellonian University in KrakowDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynAbstract Females’ reproductive capacity is closely related to the actual metabolic status of the organism. The pituitary, an element of the regulatory hypothalamic-pituitary-ovarian axis, is one of the most important endocrine glands regulating reproductive system activity. Undisturbed functioning of pituitary ensures the regular course of pregnancy through, among others, the modulation of steroid hormones production, which is critical in the early stages of gestation. Visfatin, a hormone belonging to the adipokines family, may belong to a group of factors regulating the reproductive functions in response to the female’s metabolic status. Herein we verified the hypothesis assuming a modulatory effect of visfatin on the porcine anterior pituitary transcriptome on days 15 to 16 of gestation (beginning of implantation). RNA-seq analysis of the porcine anterior pituitary cells revealed changes in the expression of 203 genes (121 up-regulated and 82 down-regulated, when compared to the non-treated controls), assigned to 325 gene ontology terms. The presence of visfatin affected the frequency of alternative splicing events (194 cases), as well as long noncoding RNA expression (64 cases). Visfatin expression and the occurrence of alternative splicing events of genes that are responsible, directly or indirectly, for regulation of the secretory functions of the pituitary, including those critical for reproductive functions suggests that the adipokine may be a key agent in ensuring the appropriate hormonal milieu during the peri-implantation period.https://doi.org/10.1038/s41598-025-00766-0Anterior pituitaryEarly gestationPigRNAseqVisfatin |
| spellingShingle | Kamil Dobrzyn Grzegorz Kopij Marta Kiezun Ewa Zaobidna Marlena Gudelska Barbara Zarzecka Katarzyna Kisielewska Agnieszka Rak Nina Smolinska Tadeusz Kaminski The effect of visfatin on the transcriptomic profile of porcine anterior pituitary cells during periimplantation period Scientific Reports Anterior pituitary Early gestation Pig RNAseq Visfatin |
| title | The effect of visfatin on the transcriptomic profile of porcine anterior pituitary cells during periimplantation period |
| title_full | The effect of visfatin on the transcriptomic profile of porcine anterior pituitary cells during periimplantation period |
| title_fullStr | The effect of visfatin on the transcriptomic profile of porcine anterior pituitary cells during periimplantation period |
| title_full_unstemmed | The effect of visfatin on the transcriptomic profile of porcine anterior pituitary cells during periimplantation period |
| title_short | The effect of visfatin on the transcriptomic profile of porcine anterior pituitary cells during periimplantation period |
| title_sort | effect of visfatin on the transcriptomic profile of porcine anterior pituitary cells during periimplantation period |
| topic | Anterior pituitary Early gestation Pig RNAseq Visfatin |
| url | https://doi.org/10.1038/s41598-025-00766-0 |
| work_keys_str_mv | AT kamildobrzyn theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT grzegorzkopij theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT martakiezun theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT ewazaobidna theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT marlenagudelska theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT barbarazarzecka theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT katarzynakisielewska theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT agnieszkarak theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT ninasmolinska theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT tadeuszkaminski theeffectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT kamildobrzyn effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT grzegorzkopij effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT martakiezun effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT ewazaobidna effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT marlenagudelska effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT barbarazarzecka effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT katarzynakisielewska effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT agnieszkarak effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT ninasmolinska effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod AT tadeuszkaminski effectofvisfatinonthetranscriptomicprofileofporcineanteriorpituitarycellsduringperiimplantationperiod |