Comparative Analysis of Analytical Methods for Quantifying the Deacetylation Degree in Chitosan Polymer Chains
Chitosan has gained considerable attention in recent years as a versatile material with applications in many fields, including food, pharmaceuticals, tissue engineering, and agriculture. The degree of deacetylation (DD) is a critical parameter of the properties and functionality of chitosan, influen...
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Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)
2025-07-01
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| Series: | Materials Research |
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| author | Talita Martins Gustavo L. Oliveira Samuel B. Henriques Eduardo H.M. Nunes |
| author_facet | Talita Martins Gustavo L. Oliveira Samuel B. Henriques Eduardo H.M. Nunes |
| author_sort | Talita Martins |
| collection | DOAJ |
| description | Chitosan has gained considerable attention in recent years as a versatile material with applications in many fields, including food, pharmaceuticals, tissue engineering, and agriculture. The degree of deacetylation (DD) is a critical parameter of the properties and functionality of chitosan, influencing its solubility, bioactivity and suitability for various applications. Accurate determination of DD is crucial as it directly affects the behavior of the material in different environments. However, high-precision methods for DD measurement are often costly and difficult to access, posing a challenge for both academic research and industrial applications. In this study, we evaluated the DD of commercial chitosan using a variety of widely available techniques, including potentiometric titration (PT), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, UV-Vis spectroscopy, and X-ray diffraction (XRD). Despite observed differences between methods, all techniques effectively quantified DD, shedding light on factors such as sample solubility, moisture content, baseline correction, and instrument accuracy that contribute to these differences. By demonstrating the feasibility and reliability of simple and accessible techniques for DD determination, this study provides valuable insights for improving the use of chitosan in both research and industry, making these materials more accessible for a wide range of applications. |
| format | Article |
| id | doaj-art-5d85bd9c86aa4bde91fd46e0d00fea58 |
| institution | Kabale University |
| issn | 1516-1439 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol) |
| record_format | Article |
| series | Materials Research |
| spelling | doaj-art-5d85bd9c86aa4bde91fd46e0d00fea582025-08-20T03:51:18ZengAssociação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)Materials Research1516-14392025-07-0128suppl 110.1590/1980-5373-mr-2025-0108Comparative Analysis of Analytical Methods for Quantifying the Deacetylation Degree in Chitosan Polymer ChainsTalita Martinshttps://orcid.org/0000-0002-0309-9849Gustavo L. OliveiraSamuel B. HenriquesEduardo H.M. Nuneshttps://orcid.org/0000-0001-6653-5137Chitosan has gained considerable attention in recent years as a versatile material with applications in many fields, including food, pharmaceuticals, tissue engineering, and agriculture. The degree of deacetylation (DD) is a critical parameter of the properties and functionality of chitosan, influencing its solubility, bioactivity and suitability for various applications. Accurate determination of DD is crucial as it directly affects the behavior of the material in different environments. However, high-precision methods for DD measurement are often costly and difficult to access, posing a challenge for both academic research and industrial applications. In this study, we evaluated the DD of commercial chitosan using a variety of widely available techniques, including potentiometric titration (PT), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, UV-Vis spectroscopy, and X-ray diffraction (XRD). Despite observed differences between methods, all techniques effectively quantified DD, shedding light on factors such as sample solubility, moisture content, baseline correction, and instrument accuracy that contribute to these differences. By demonstrating the feasibility and reliability of simple and accessible techniques for DD determination, this study provides valuable insights for improving the use of chitosan in both research and industry, making these materials more accessible for a wide range of applications.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392025000200264&lng=en&tlng=enChitosanDegree of DeacetylationPotentiometric titrationSpectroscopy methodsX-ray diffraction |
| spellingShingle | Talita Martins Gustavo L. Oliveira Samuel B. Henriques Eduardo H.M. Nunes Comparative Analysis of Analytical Methods for Quantifying the Deacetylation Degree in Chitosan Polymer Chains Materials Research Chitosan Degree of Deacetylation Potentiometric titration Spectroscopy methods X-ray diffraction |
| title | Comparative Analysis of Analytical Methods for Quantifying the Deacetylation Degree in Chitosan Polymer Chains |
| title_full | Comparative Analysis of Analytical Methods for Quantifying the Deacetylation Degree in Chitosan Polymer Chains |
| title_fullStr | Comparative Analysis of Analytical Methods for Quantifying the Deacetylation Degree in Chitosan Polymer Chains |
| title_full_unstemmed | Comparative Analysis of Analytical Methods for Quantifying the Deacetylation Degree in Chitosan Polymer Chains |
| title_short | Comparative Analysis of Analytical Methods for Quantifying the Deacetylation Degree in Chitosan Polymer Chains |
| title_sort | comparative analysis of analytical methods for quantifying the deacetylation degree in chitosan polymer chains |
| topic | Chitosan Degree of Deacetylation Potentiometric titration Spectroscopy methods X-ray diffraction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392025000200264&lng=en&tlng=en |
| work_keys_str_mv | AT talitamartins comparativeanalysisofanalyticalmethodsforquantifyingthedeacetylationdegreeinchitosanpolymerchains AT gustavololiveira comparativeanalysisofanalyticalmethodsforquantifyingthedeacetylationdegreeinchitosanpolymerchains AT samuelbhenriques comparativeanalysisofanalyticalmethodsforquantifyingthedeacetylationdegreeinchitosanpolymerchains AT eduardohmnunes comparativeanalysisofanalyticalmethodsforquantifyingthedeacetylationdegreeinchitosanpolymerchains |