Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish
Follicles are the basic structure and functional unit of ovaries. In the previous study, we classified primary follicles (PFs) of zebrafish into four subtypes, compared the DNA methylation and RNA expression characteristics among different subtypes follicles, and preliminarily analyzed the molecular...
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KeAi Communications Co. Ltd.
2025-06-01
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| Series: | Reproduction and Breeding |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667071225000122 |
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| author | Guangjing Zhang Jing Huang Liangyue Peng Jinhui Liu Wenbin Liu Wen Fu |
| author_facet | Guangjing Zhang Jing Huang Liangyue Peng Jinhui Liu Wenbin Liu Wen Fu |
| author_sort | Guangjing Zhang |
| collection | DOAJ |
| description | Follicles are the basic structure and functional unit of ovaries. In the previous study, we classified primary follicles (PFs) of zebrafish into four subtypes, compared the DNA methylation and RNA expression characteristics among different subtypes follicles, and preliminarily analyzed the molecular mechanism of PFs development. However, the protein expression characteristics of the four subtypes of PFs remain poorly understood. In this study, a total of 9635 proteins were identified in the four subtypes PFs of zebrafish by proteomic analysis. The proteins highly expressed in the PF-ii and PF-iii were enriched in Notch, MAPK, and mTOR signaling pathways. By combined transcriptomic and proteomic analysis screened five key candidate molecules involved in PFs developmental regulation. These results provide reference to the molecular mechanisms underlying follicular development in fish. |
| format | Article |
| id | doaj-art-5d7fb54d046748f993e784bc5b911b39 |
| institution | OA Journals |
| issn | 2667-0712 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Reproduction and Breeding |
| spelling | doaj-art-5d7fb54d046748f993e784bc5b911b392025-08-20T02:26:14ZengKeAi Communications Co. Ltd.Reproduction and Breeding2667-07122025-06-0152212610.1016/j.repbre.2025.03.001Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafishGuangjing Zhang0Jing Huang1Liangyue Peng2Jinhui Liu3Wenbin Liu4Wen Fu5State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, PR China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, PR China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, PR China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, PR China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, PR China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, Hunan, 410081, PR China; College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, PR China; Corresponding author. Engineering Research Center of Ployploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, PR China.Follicles are the basic structure and functional unit of ovaries. In the previous study, we classified primary follicles (PFs) of zebrafish into four subtypes, compared the DNA methylation and RNA expression characteristics among different subtypes follicles, and preliminarily analyzed the molecular mechanism of PFs development. However, the protein expression characteristics of the four subtypes of PFs remain poorly understood. In this study, a total of 9635 proteins were identified in the four subtypes PFs of zebrafish by proteomic analysis. The proteins highly expressed in the PF-ii and PF-iii were enriched in Notch, MAPK, and mTOR signaling pathways. By combined transcriptomic and proteomic analysis screened five key candidate molecules involved in PFs developmental regulation. These results provide reference to the molecular mechanisms underlying follicular development in fish.http://www.sciencedirect.com/science/article/pii/S2667071225000122Primary folliclesProteomicDifferentially expressed proteinsZebrafish |
| spellingShingle | Guangjing Zhang Jing Huang Liangyue Peng Jinhui Liu Wenbin Liu Wen Fu Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish Reproduction and Breeding Primary follicles Proteomic Differentially expressed proteins Zebrafish |
| title | Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish |
| title_full | Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish |
| title_fullStr | Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish |
| title_full_unstemmed | Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish |
| title_short | Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish |
| title_sort | quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish |
| topic | Primary follicles Proteomic Differentially expressed proteins Zebrafish |
| url | http://www.sciencedirect.com/science/article/pii/S2667071225000122 |
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