Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli Infection
The present study was designed to determine the relationships among biofilm formation, cellular stress and release of Shiga toxin (Stx) by three different clinical Shiga toxin-producing Escherichia coli (STEC) strains. The biofilm formation was determined using crystal violet stain in tryptic soy br...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/607258 |
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| author | Natalia Angel Villegas José Baronetti Inés Albesa Rosana Polifroni Alberto Parma Analía Etcheverría Maria Becerra Nora Padola Maria Paraje |
| author_facet | Natalia Angel Villegas José Baronetti Inés Albesa Rosana Polifroni Alberto Parma Analía Etcheverría Maria Becerra Nora Padola Maria Paraje |
| author_sort | Natalia Angel Villegas |
| collection | DOAJ |
| description | The present study was designed to determine the relationships among biofilm formation, cellular stress and release of Shiga toxin (Stx) by three different clinical Shiga toxin-producing Escherichia coli (STEC) strains. The biofilm formation was determined using crystal violet stain in tryptic soy broth or thioglycollate medium with the addition of sugars (glucose or mannose) or hydrogen peroxide. The reactive oxygen species (ROSs) were detected by the reduction of nitro blue tetrazolium and reactive nitrogen intermediates (RNI) determined by the Griess assay. In addition, the activities of two antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were studied. For the cytotoxicity studies, Vero cells were cultured with Stx released of STEC biofilms. The addition of sugars in both culture mediums resulted in an increase in biofilm biomass, with a decrease in ROS and RNI production, low levels of SOD and CAT activity, and minimal cytotoxic effects. However, under stressful conditions, an important increase in the antioxidant enzyme activity and high level of Stx production were observed. The disturbance in the prooxidant-antioxidant balance and its effect on the production and release of Stx evaluated under different conditions of biofilm formation may contribute to a better understanding of the relevance of biofilms in the pathogenesis of STEC infection. |
| format | Article |
| id | doaj-art-5d632a7dc2e94ead9c6d5a0ba4601e6b |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-5d632a7dc2e94ead9c6d5a0ba4601e6b2025-08-20T03:34:45ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/607258607258Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli InfectionNatalia Angel Villegas0José Baronetti1Inés Albesa2Rosana Polifroni3Alberto Parma4Analía Etcheverría5Maria Becerra6Nora Padola7Maria Paraje8IMBIV, CONICET y Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, 5000 Córdoba, ArgentinaIMBIV, CONICET y Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, 5000 Córdoba, ArgentinaIMBIV, CONICET y Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, 5000 Córdoba, ArgentinaLaboratorio de Inmunoquímica y Biotecnología, Departamento de SAMP, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, ArgentinaLaboratorio de Inmunoquímica y Biotecnología, Departamento de SAMP, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, ArgentinaLaboratorio de Inmunoquímica y Biotecnología, Departamento de SAMP, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, ArgentinaIMBIV, CONICET y Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, 5000 Córdoba, ArgentinaLaboratorio de Inmunoquímica y Biotecnología, Departamento de SAMP, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, ArgentinaIMBIV, CONICET y Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, 5000 Córdoba, ArgentinaThe present study was designed to determine the relationships among biofilm formation, cellular stress and release of Shiga toxin (Stx) by three different clinical Shiga toxin-producing Escherichia coli (STEC) strains. The biofilm formation was determined using crystal violet stain in tryptic soy broth or thioglycollate medium with the addition of sugars (glucose or mannose) or hydrogen peroxide. The reactive oxygen species (ROSs) were detected by the reduction of nitro blue tetrazolium and reactive nitrogen intermediates (RNI) determined by the Griess assay. In addition, the activities of two antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were studied. For the cytotoxicity studies, Vero cells were cultured with Stx released of STEC biofilms. The addition of sugars in both culture mediums resulted in an increase in biofilm biomass, with a decrease in ROS and RNI production, low levels of SOD and CAT activity, and minimal cytotoxic effects. However, under stressful conditions, an important increase in the antioxidant enzyme activity and high level of Stx production were observed. The disturbance in the prooxidant-antioxidant balance and its effect on the production and release of Stx evaluated under different conditions of biofilm formation may contribute to a better understanding of the relevance of biofilms in the pathogenesis of STEC infection.http://dx.doi.org/10.1155/2013/607258 |
| spellingShingle | Natalia Angel Villegas José Baronetti Inés Albesa Rosana Polifroni Alberto Parma Analía Etcheverría Maria Becerra Nora Padola Maria Paraje Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli Infection The Scientific World Journal |
| title | Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli Infection |
| title_full | Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli Infection |
| title_fullStr | Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli Infection |
| title_full_unstemmed | Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli Infection |
| title_short | Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli Infection |
| title_sort | relevance of biofilms in the pathogenesis of shiga toxin producing escherichia coli infection |
| url | http://dx.doi.org/10.1155/2013/607258 |
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