Effects of Zeaxanthin on Growth and Invasion of Human Uveal Melanoma in Nude Mouse Model
Uveal melanoma cells were inoculated into the choroid of nude mice and treated with or without intraocular injection of zeaxanthin. After 21 days, mice were sacrificed and the eyes enucleated. Histopathological analysis was performed in hematoxylin and eosin stained frozen sections. Melanoma develop...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2015/392305 |
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| author | Xiaoliang L. Xu Dan-Ning Hu Codrin Iacob Adrienne Jordan Sandipkumar Gandhi Dennis L. Gierhart Richard Rosen |
| author_facet | Xiaoliang L. Xu Dan-Ning Hu Codrin Iacob Adrienne Jordan Sandipkumar Gandhi Dennis L. Gierhart Richard Rosen |
| author_sort | Xiaoliang L. Xu |
| collection | DOAJ |
| description | Uveal melanoma cells were inoculated into the choroid of nude mice and treated with or without intraocular injection of zeaxanthin. After 21 days, mice were sacrificed and the eyes enucleated. Histopathological analysis was performed in hematoxylin and eosin stained frozen sections. Melanoma developed rapidly in the control group (without treatment of zeaxanthin). Tumor-bearing eye mass and tumor mass in the control group were significantly greater than those in zeaxanthin treated group. Melanoma in the controlled eyes occupied a large part of the eye, was epithelioid in morphology, and was with numerous mitotic figures. Scleral perforation and extraocular extension were observed in half of the eyes. Melanomas in zeaxanthin treated eyes were significantly smaller with many necrosis and apoptosis areas and no extraocular extension could be found. Quantitative image analysis revealed that the tumor size was reduced by 56% in eyes treated with low dosages of zeaxanthin and 92% in eyes treatment with high dosages of zeaxanthin, as compared to the controls. This study demonstrated that zeaxanthin significantly inhibits the growth and invasion of human uveal melanoma in nude mice, suggesting that zeaxanthin may be a promising agent to be explored for the prevention and treatment of uveal melanoma. |
| format | Article |
| id | doaj-art-5d4c102049cc45f395e02da76fe9d201 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-5d4c102049cc45f395e02da76fe9d2012025-08-20T03:34:44ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/392305392305Effects of Zeaxanthin on Growth and Invasion of Human Uveal Melanoma in Nude Mouse ModelXiaoliang L. Xu0Dan-Ning Hu1Codrin Iacob2Adrienne Jordan3Sandipkumar Gandhi4Dennis L. Gierhart5Richard Rosen6Department of Pathology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USADepartment of Ophthalmology, The New York Eye and Ear Infirmary of Mount Sinai, Icahn School of Medicine at Mount Sinai, New York, NY 10003, USADepartment of Pathology, The New York Eye and Ear Infirmary of Mount Sinai, Icahn School of Medicine at Mount Sinai, New York, NY 10003, USADepartment of Pathology, The New York Eye and Ear Infirmary of Mount Sinai, Icahn School of Medicine at Mount Sinai, New York, NY 10003, USADepartment of Pathology, The New York Eye and Ear Infirmary of Mount Sinai, Icahn School of Medicine at Mount Sinai, New York, NY 10003, USAZeaVision LLC, Chesterfield, MO 63005, USADepartment of Ophthalmology, The New York Eye and Ear Infirmary of Mount Sinai, Icahn School of Medicine at Mount Sinai, New York, NY 10003, USAUveal melanoma cells were inoculated into the choroid of nude mice and treated with or without intraocular injection of zeaxanthin. After 21 days, mice were sacrificed and the eyes enucleated. Histopathological analysis was performed in hematoxylin and eosin stained frozen sections. Melanoma developed rapidly in the control group (without treatment of zeaxanthin). Tumor-bearing eye mass and tumor mass in the control group were significantly greater than those in zeaxanthin treated group. Melanoma in the controlled eyes occupied a large part of the eye, was epithelioid in morphology, and was with numerous mitotic figures. Scleral perforation and extraocular extension were observed in half of the eyes. Melanomas in zeaxanthin treated eyes were significantly smaller with many necrosis and apoptosis areas and no extraocular extension could be found. Quantitative image analysis revealed that the tumor size was reduced by 56% in eyes treated with low dosages of zeaxanthin and 92% in eyes treatment with high dosages of zeaxanthin, as compared to the controls. This study demonstrated that zeaxanthin significantly inhibits the growth and invasion of human uveal melanoma in nude mice, suggesting that zeaxanthin may be a promising agent to be explored for the prevention and treatment of uveal melanoma.http://dx.doi.org/10.1155/2015/392305 |
| spellingShingle | Xiaoliang L. Xu Dan-Ning Hu Codrin Iacob Adrienne Jordan Sandipkumar Gandhi Dennis L. Gierhart Richard Rosen Effects of Zeaxanthin on Growth and Invasion of Human Uveal Melanoma in Nude Mouse Model Journal of Ophthalmology |
| title | Effects of Zeaxanthin on Growth and Invasion of Human Uveal Melanoma in Nude Mouse Model |
| title_full | Effects of Zeaxanthin on Growth and Invasion of Human Uveal Melanoma in Nude Mouse Model |
| title_fullStr | Effects of Zeaxanthin on Growth and Invasion of Human Uveal Melanoma in Nude Mouse Model |
| title_full_unstemmed | Effects of Zeaxanthin on Growth and Invasion of Human Uveal Melanoma in Nude Mouse Model |
| title_short | Effects of Zeaxanthin on Growth and Invasion of Human Uveal Melanoma in Nude Mouse Model |
| title_sort | effects of zeaxanthin on growth and invasion of human uveal melanoma in nude mouse model |
| url | http://dx.doi.org/10.1155/2015/392305 |
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