Sensitive Spectrophotometric Methods for the Determination of Gatifloxacin in Pharmaceuticals Using Bromate-Bromide, Methylene Blue and Rhodamine-B as Reagents
Two new simple, precise, rapid and extraction-free spectrophotometric methods are proposed for the determination of gatifloxacin(GTF) using bromate-bromide mixture and two dyes, methylene blue and rhodamine B, as reagents. Spectrophotometric methods entail the addition of a known excess of bromate-b...
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2007/374837 |
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| author | K. Basavaiah U. R. Anil Kumar |
| author_facet | K. Basavaiah U. R. Anil Kumar |
| author_sort | K. Basavaiah |
| collection | DOAJ |
| description | Two new simple, precise, rapid and extraction-free spectrophotometric methods are proposed for the determination of gatifloxacin(GTF) using bromate-bromide mixture and two dyes, methylene blue and rhodamine B, as reagents. Spectrophotometric methods entail the addition of a known excess of bromate-bromide mixture to GTF in hydrochloric acid medium followed by determination of residual bromine by reacting with a fixed amount of either methylene blue and measuring the absorbance at 665 nm (Method A) or rhodamine B and measuring the absorbance at 555 nm (Method B). Beer᾽s law is obeyed in the ranges, 0.5-5.0 and 0.2-1.5 μg mL-1 for method A and method B, respectively. The apparent molar absorptivities are calculated to be 5.6×104 and 9.6×104 L mol-1cm-1 for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.0071 and 0.0042 μg cm-2. The methods were successfully applied to the assay of GTF in pharmaceutical formulations with satisfactory results. |
| format | Article |
| id | doaj-art-5d4bc2c52f7841818afbd20e8b2877b9 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-5d4bc2c52f7841818afbd20e8b2877b92025-02-03T01:28:01ZengWileyE-Journal of Chemistry0973-49452090-98102007-01-014215416110.1155/2007/374837Sensitive Spectrophotometric Methods for the Determination of Gatifloxacin in Pharmaceuticals Using Bromate-Bromide, Methylene Blue and Rhodamine-B as ReagentsK. Basavaiah0U. R. Anil Kumar1Department of Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, IndiaDepartment of Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, IndiaTwo new simple, precise, rapid and extraction-free spectrophotometric methods are proposed for the determination of gatifloxacin(GTF) using bromate-bromide mixture and two dyes, methylene blue and rhodamine B, as reagents. Spectrophotometric methods entail the addition of a known excess of bromate-bromide mixture to GTF in hydrochloric acid medium followed by determination of residual bromine by reacting with a fixed amount of either methylene blue and measuring the absorbance at 665 nm (Method A) or rhodamine B and measuring the absorbance at 555 nm (Method B). Beer᾽s law is obeyed in the ranges, 0.5-5.0 and 0.2-1.5 μg mL-1 for method A and method B, respectively. The apparent molar absorptivities are calculated to be 5.6×104 and 9.6×104 L mol-1cm-1 for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.0071 and 0.0042 μg cm-2. The methods were successfully applied to the assay of GTF in pharmaceutical formulations with satisfactory results.http://dx.doi.org/10.1155/2007/374837 |
| spellingShingle | K. Basavaiah U. R. Anil Kumar Sensitive Spectrophotometric Methods for the Determination of Gatifloxacin in Pharmaceuticals Using Bromate-Bromide, Methylene Blue and Rhodamine-B as Reagents E-Journal of Chemistry |
| title | Sensitive Spectrophotometric Methods for the Determination of Gatifloxacin in Pharmaceuticals Using Bromate-Bromide, Methylene Blue and Rhodamine-B as Reagents |
| title_full | Sensitive Spectrophotometric Methods for the Determination of Gatifloxacin in Pharmaceuticals Using Bromate-Bromide, Methylene Blue and Rhodamine-B as Reagents |
| title_fullStr | Sensitive Spectrophotometric Methods for the Determination of Gatifloxacin in Pharmaceuticals Using Bromate-Bromide, Methylene Blue and Rhodamine-B as Reagents |
| title_full_unstemmed | Sensitive Spectrophotometric Methods for the Determination of Gatifloxacin in Pharmaceuticals Using Bromate-Bromide, Methylene Blue and Rhodamine-B as Reagents |
| title_short | Sensitive Spectrophotometric Methods for the Determination of Gatifloxacin in Pharmaceuticals Using Bromate-Bromide, Methylene Blue and Rhodamine-B as Reagents |
| title_sort | sensitive spectrophotometric methods for the determination of gatifloxacin in pharmaceuticals using bromate bromide methylene blue and rhodamine b as reagents |
| url | http://dx.doi.org/10.1155/2007/374837 |
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