Dichrocephala integrifolia (L.f.) Kuntze Leaf Aqueous Extract Improves Liver Architecture in a Model of Aflatoxin-Containing Peanut-Induced Hepatotoxicity in Wistar Rats
Consumption of aflatoxin-contaminated food is responsible for hepatotoxicity. Dichrocephala integrifolia (D. integrifolia) is used in traditional African medicine to treat various liver diseases. This study aimed to evaluate the therapeutic effects of aqueous leaf extract of D. integrifolia on aflat...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/jt/5551651 |
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| author | Adolphe Mbatchou Florence Ngueguim Tsofack Jean Hubert Donfack Raceline Kamkumo Gounoue Michel Arnaud Mbock Jean Philippe Tientcheu Djientcheu Franklin Gamo Zemo Rodrigue Ngapout Fifen Paul Desire Djomeni Dzeufiet Théophile Dimo |
| author_facet | Adolphe Mbatchou Florence Ngueguim Tsofack Jean Hubert Donfack Raceline Kamkumo Gounoue Michel Arnaud Mbock Jean Philippe Tientcheu Djientcheu Franklin Gamo Zemo Rodrigue Ngapout Fifen Paul Desire Djomeni Dzeufiet Théophile Dimo |
| author_sort | Adolphe Mbatchou |
| collection | DOAJ |
| description | Consumption of aflatoxin-contaminated food is responsible for hepatotoxicity. Dichrocephala integrifolia (D. integrifolia) is used in traditional African medicine to treat various liver diseases. This study aimed to evaluate the therapeutic effects of aqueous leaf extract of D. integrifolia on aflatoxin-containing peanut-induced liver damage in Wistar rats. The animals were fed the standard diet (SD) or the SD supplemented with stored and poorly preserved peanuts (50:50) containing aflatoxins for 42 days. Then, animals received the diet concomitantly with D. integrifolia (100, 200 mg/kg), or Silybon 100 mg/kg for 14 days. At the end of the experimental period, biochemical markers, including the lipid profile, liver function markers, and proinflammatory markers, were evaluated. A histopathological analysis of the liver was performed. Semiquantitative evaluation of aflatoxin B1 in peanuts by thin-layer chromatography was carried out, and phytochemical characterization of the extract by high-performance liquid chromatography–mass spectrometry was performed. As a result, poorly stored peanuts contain 20 μg/kg aflatoxin B1/kg of peanuts. Consumption of contaminated peanuts resulted in inflammation characterized by a significant increase (p<0.001) in proinflammatory cytokines (TNF-α, INF-γ, and IL-13) and a significant increase (p<0.001) in transaminase activities, GGT, ALP, and levels of total bilirubin, total cholesterol, triglycerides, LDL cholesterol, and a significant decrease (p<0.001) in HDL cholesterol and albumin levels. These various abnormalities were accompanied by a significant increase (p<0.001) in oxidative stress. These disturbances were confirmed by hepatic cytolysis, leukocyte infiltration, and vascular congestion. Treatment with D. integrifolia extract at all doses tested reversed these abnormalities. These beneficial effects of the extract could be due to β-amyrin formate, identified in this extract, and could therefore justify the use of this plant extract in traditional medicine to manage liver diseases. |
| format | Article |
| id | doaj-art-5d4263386b3a4e728c41bb3ded81effd |
| institution | OA Journals |
| issn | 1687-8205 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Journal of Toxicology |
| spelling | doaj-art-5d4263386b3a4e728c41bb3ded81effd2025-08-20T01:56:48ZengWileyJournal of Toxicology1687-82052025-01-01202510.1155/jt/5551651Dichrocephala integrifolia (L.f.) Kuntze Leaf Aqueous Extract Improves Liver Architecture in a Model of Aflatoxin-Containing Peanut-Induced Hepatotoxicity in Wistar RatsAdolphe Mbatchou0Florence Ngueguim Tsofack1Jean Hubert Donfack2Raceline Kamkumo Gounoue3Michel Arnaud Mbock4Jean Philippe Tientcheu Djientcheu5Franklin Gamo Zemo6Rodrigue Ngapout Fifen7Paul Desire Djomeni Dzeufiet8Théophile Dimo9Department of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyDepartment of Pharmaceutical SciencesDepartment of Animal Biology and PhysiologyDepartment of BiochemistryDepartment of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyConsumption of aflatoxin-contaminated food is responsible for hepatotoxicity. Dichrocephala integrifolia (D. integrifolia) is used in traditional African medicine to treat various liver diseases. This study aimed to evaluate the therapeutic effects of aqueous leaf extract of D. integrifolia on aflatoxin-containing peanut-induced liver damage in Wistar rats. The animals were fed the standard diet (SD) or the SD supplemented with stored and poorly preserved peanuts (50:50) containing aflatoxins for 42 days. Then, animals received the diet concomitantly with D. integrifolia (100, 200 mg/kg), or Silybon 100 mg/kg for 14 days. At the end of the experimental period, biochemical markers, including the lipid profile, liver function markers, and proinflammatory markers, were evaluated. A histopathological analysis of the liver was performed. Semiquantitative evaluation of aflatoxin B1 in peanuts by thin-layer chromatography was carried out, and phytochemical characterization of the extract by high-performance liquid chromatography–mass spectrometry was performed. As a result, poorly stored peanuts contain 20 μg/kg aflatoxin B1/kg of peanuts. Consumption of contaminated peanuts resulted in inflammation characterized by a significant increase (p<0.001) in proinflammatory cytokines (TNF-α, INF-γ, and IL-13) and a significant increase (p<0.001) in transaminase activities, GGT, ALP, and levels of total bilirubin, total cholesterol, triglycerides, LDL cholesterol, and a significant decrease (p<0.001) in HDL cholesterol and albumin levels. These various abnormalities were accompanied by a significant increase (p<0.001) in oxidative stress. These disturbances were confirmed by hepatic cytolysis, leukocyte infiltration, and vascular congestion. Treatment with D. integrifolia extract at all doses tested reversed these abnormalities. These beneficial effects of the extract could be due to β-amyrin formate, identified in this extract, and could therefore justify the use of this plant extract in traditional medicine to manage liver diseases.http://dx.doi.org/10.1155/jt/5551651 |
| spellingShingle | Adolphe Mbatchou Florence Ngueguim Tsofack Jean Hubert Donfack Raceline Kamkumo Gounoue Michel Arnaud Mbock Jean Philippe Tientcheu Djientcheu Franklin Gamo Zemo Rodrigue Ngapout Fifen Paul Desire Djomeni Dzeufiet Théophile Dimo Dichrocephala integrifolia (L.f.) Kuntze Leaf Aqueous Extract Improves Liver Architecture in a Model of Aflatoxin-Containing Peanut-Induced Hepatotoxicity in Wistar Rats Journal of Toxicology |
| title | Dichrocephala integrifolia (L.f.) Kuntze Leaf Aqueous Extract Improves Liver Architecture in a Model of Aflatoxin-Containing Peanut-Induced Hepatotoxicity in Wistar Rats |
| title_full | Dichrocephala integrifolia (L.f.) Kuntze Leaf Aqueous Extract Improves Liver Architecture in a Model of Aflatoxin-Containing Peanut-Induced Hepatotoxicity in Wistar Rats |
| title_fullStr | Dichrocephala integrifolia (L.f.) Kuntze Leaf Aqueous Extract Improves Liver Architecture in a Model of Aflatoxin-Containing Peanut-Induced Hepatotoxicity in Wistar Rats |
| title_full_unstemmed | Dichrocephala integrifolia (L.f.) Kuntze Leaf Aqueous Extract Improves Liver Architecture in a Model of Aflatoxin-Containing Peanut-Induced Hepatotoxicity in Wistar Rats |
| title_short | Dichrocephala integrifolia (L.f.) Kuntze Leaf Aqueous Extract Improves Liver Architecture in a Model of Aflatoxin-Containing Peanut-Induced Hepatotoxicity in Wistar Rats |
| title_sort | dichrocephala integrifolia l f kuntze leaf aqueous extract improves liver architecture in a model of aflatoxin containing peanut induced hepatotoxicity in wistar rats |
| url | http://dx.doi.org/10.1155/jt/5551651 |
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