Efficient Two-Stage Meso- and Thermophilic Anaerobic Digestion of Food Waste from a Microbial Perspective
Two-stage meso- and thermophilic anaerobic digestion (TSMTAD) of food waste was examined and its microbiological structure was investigated. The first stage was designed for the primary storage of perishable food waste and the second stage for central biogas production. Mesophilic storage with initi...
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2024-11-01
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| author | Katsuaki Ohdoi Yoshihiro Okamoto Tomonori Koga Haruka Takahashi Mugihito Oshiro Toshihito Morimitsu Hideki Muraoka Yukihiro Tashiro Kenji Sakai |
| author_facet | Katsuaki Ohdoi Yoshihiro Okamoto Tomonori Koga Haruka Takahashi Mugihito Oshiro Toshihito Morimitsu Hideki Muraoka Yukihiro Tashiro Kenji Sakai |
| author_sort | Katsuaki Ohdoi |
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| description | Two-stage meso- and thermophilic anaerobic digestion (TSMTAD) of food waste was examined and its microbiological structure was investigated. The first stage was designed for the primary storage of perishable food waste and the second stage for central biogas production. Mesophilic storage with initial neutralization and inoculation of lactic acid bacteria (LAB) resulted in an accumulation of lactic acid (21–23 g/L) with a decreased pH, in which bacterial members in facultative hetero-fermentation-type LAB dominated. Repeated fed-batch storage showed stable accumulation of lactic acid, retaining 89.3% (av.) carbon and preventing the growth of exogenous food pathogens. When the second stage of TSMTAD was compared with direct single-stage anaerobic digestion (SSAD) at 55 °C, the amount of methane accumulated was 1.48-fold higher (896 NmL/g-vs.). The methane yield of the original food refuse was 6.9% higher in the case of TSMTAD. The microbial community structures of both cases were similar, consisting of a sole thermophilic hydrogen-assimilating methanogen, <i>Methanothermobacter thermautotrophicus</i>. However, the abundance of bacteria belonging to two functional groups, H<sub>2</sub> CO<sub>2</sub> and acetic acid producer, and syntrophic acetate-oxidizing bacteria increased in TSMTAD. This may change the metabolic pathway, contributing to the stimulation of methane productivity. |
| format | Article |
| id | doaj-art-5d362befcf7b40e6965ab5e654fb42dd |
| institution | DOAJ |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Fermentation |
| spelling | doaj-art-5d362befcf7b40e6965ab5e654fb42dd2025-08-20T02:55:32ZengMDPI AGFermentation2311-56372024-11-01101260710.3390/fermentation10120607Efficient Two-Stage Meso- and Thermophilic Anaerobic Digestion of Food Waste from a Microbial PerspectiveKatsuaki Ohdoi0Yoshihiro Okamoto1Tomonori Koga2Haruka Takahashi3Mugihito Oshiro4Toshihito Morimitsu5Hideki Muraoka6Yukihiro Tashiro7Kenji Sakai8Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, JapanGraduate School of Bioresource and Bioenvironmental Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanGraduate School of Bioresource and Bioenvironmental Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanGraduate School of Bioresource and Bioenvironmental Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanGraduate School of Bioresource and Bioenvironmental Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanMomoyama Recycling Center R&D Division, Vioce Co., Ltd., 2822-6 Tsukatsuki, Momoyama-cho, Kinokawa 649-6112, JapanMomoyama Recycling Center R&D Division, Vioce Co., Ltd., 2822-6 Tsukatsuki, Momoyama-cho, Kinokawa 649-6112, JapanGraduate School of Bioresource and Bioenvironmental Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanGraduate School of Bioresource and Bioenvironmental Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanTwo-stage meso- and thermophilic anaerobic digestion (TSMTAD) of food waste was examined and its microbiological structure was investigated. The first stage was designed for the primary storage of perishable food waste and the second stage for central biogas production. Mesophilic storage with initial neutralization and inoculation of lactic acid bacteria (LAB) resulted in an accumulation of lactic acid (21–23 g/L) with a decreased pH, in which bacterial members in facultative hetero-fermentation-type LAB dominated. Repeated fed-batch storage showed stable accumulation of lactic acid, retaining 89.3% (av.) carbon and preventing the growth of exogenous food pathogens. When the second stage of TSMTAD was compared with direct single-stage anaerobic digestion (SSAD) at 55 °C, the amount of methane accumulated was 1.48-fold higher (896 NmL/g-vs.). The methane yield of the original food refuse was 6.9% higher in the case of TSMTAD. The microbial community structures of both cases were similar, consisting of a sole thermophilic hydrogen-assimilating methanogen, <i>Methanothermobacter thermautotrophicus</i>. However, the abundance of bacteria belonging to two functional groups, H<sub>2</sub> CO<sub>2</sub> and acetic acid producer, and syntrophic acetate-oxidizing bacteria increased in TSMTAD. This may change the metabolic pathway, contributing to the stimulation of methane productivity.https://www.mdpi.com/2311-5637/10/12/607food wasteanaerobic digestionbiogas productiontwo-stage meso- and thermophilic anaerobic digestion (TSMTAD)two-stage anaerobic digestion (TSAD)methane production |
| spellingShingle | Katsuaki Ohdoi Yoshihiro Okamoto Tomonori Koga Haruka Takahashi Mugihito Oshiro Toshihito Morimitsu Hideki Muraoka Yukihiro Tashiro Kenji Sakai Efficient Two-Stage Meso- and Thermophilic Anaerobic Digestion of Food Waste from a Microbial Perspective Fermentation food waste anaerobic digestion biogas production two-stage meso- and thermophilic anaerobic digestion (TSMTAD) two-stage anaerobic digestion (TSAD) methane production |
| title | Efficient Two-Stage Meso- and Thermophilic Anaerobic Digestion of Food Waste from a Microbial Perspective |
| title_full | Efficient Two-Stage Meso- and Thermophilic Anaerobic Digestion of Food Waste from a Microbial Perspective |
| title_fullStr | Efficient Two-Stage Meso- and Thermophilic Anaerobic Digestion of Food Waste from a Microbial Perspective |
| title_full_unstemmed | Efficient Two-Stage Meso- and Thermophilic Anaerobic Digestion of Food Waste from a Microbial Perspective |
| title_short | Efficient Two-Stage Meso- and Thermophilic Anaerobic Digestion of Food Waste from a Microbial Perspective |
| title_sort | efficient two stage meso and thermophilic anaerobic digestion of food waste from a microbial perspective |
| topic | food waste anaerobic digestion biogas production two-stage meso- and thermophilic anaerobic digestion (TSMTAD) two-stage anaerobic digestion (TSAD) methane production |
| url | https://www.mdpi.com/2311-5637/10/12/607 |
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