Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles
Abstract The increasing use of nanoparticles in numerous applications has led to growing concern about their potential toxicological properties. However, the role of epigenetic alterations in toxicity due to nanoparticles remains relatively unexplored. In this study, we examined the effects of ten k...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-07206-z |
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| author | Xiaoxu Zhao Liping Zhao Hui Lin Xiumei Wang |
| author_facet | Xiaoxu Zhao Liping Zhao Hui Lin Xiumei Wang |
| author_sort | Xiaoxu Zhao |
| collection | DOAJ |
| description | Abstract The increasing use of nanoparticles in numerous applications has led to growing concern about their potential toxicological properties. However, the role of epigenetic alterations in toxicity due to nanoparticles remains relatively unexplored. In this study, we examined the effects of ten kinds of nanoparticles on histone modification. We observed that Al2O3, CuO or ZnO nanoparticles induced phosphorylation of the histone H2AX at serine 139. In addition, compared with other tested nanoparticles, acetylation of histone H3 lysine 9, 14 and histone H3 (global) caused by CuO and ZnO nanoparticles was most significant. We found that upregulation of histone H3 lysine 27 trimethylation was highly correlated with histone acetylation induced by CuO and ZnO nanoparticles. Furthermore, we proved that CuO or ZnO nanoparticle-induced histone H3 modifications may occur via release of ions from nanoparticles inside cells. We previously showed that phosphorylation of histone H3 at serine 10 can be used to evaluate the toxicity of silver nanoparticles and Ag ion release in combination with detection by side-scattered light from flow cytometry. Our current findings suggest that other histone modifications such as acetylation and methylation of histone H3, may also be good markers for nanoparticle toxicity. |
| format | Article |
| id | doaj-art-5d2297572ccf47baaefa4bf0d7a95a7a |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-5d2297572ccf47baaefa4bf0d7a95a7a2025-08-20T03:45:30ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-07206-zHistone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticlesXiaoxu Zhao0Liping Zhao1Hui Lin2Xiumei Wang3Fujian Provincial Key Laboratory of Ecology-Toxicological Effects & Control for Emerging Contaminants, Putian UniversityCollege of Environmental and Biological Engineering, Putian UniversityCollege of Environmental and Biological Engineering, Putian UniversityCollege of Environmental and Biological Engineering, Putian UniversityAbstract The increasing use of nanoparticles in numerous applications has led to growing concern about their potential toxicological properties. However, the role of epigenetic alterations in toxicity due to nanoparticles remains relatively unexplored. In this study, we examined the effects of ten kinds of nanoparticles on histone modification. We observed that Al2O3, CuO or ZnO nanoparticles induced phosphorylation of the histone H2AX at serine 139. In addition, compared with other tested nanoparticles, acetylation of histone H3 lysine 9, 14 and histone H3 (global) caused by CuO and ZnO nanoparticles was most significant. We found that upregulation of histone H3 lysine 27 trimethylation was highly correlated with histone acetylation induced by CuO and ZnO nanoparticles. Furthermore, we proved that CuO or ZnO nanoparticle-induced histone H3 modifications may occur via release of ions from nanoparticles inside cells. We previously showed that phosphorylation of histone H3 at serine 10 can be used to evaluate the toxicity of silver nanoparticles and Ag ion release in combination with detection by side-scattered light from flow cytometry. Our current findings suggest that other histone modifications such as acetylation and methylation of histone H3, may also be good markers for nanoparticle toxicity.https://doi.org/10.1038/s41598-025-07206-zNanoparticlesEpigeneticHistone modificationIon releaseIntracellular accumulation |
| spellingShingle | Xiaoxu Zhao Liping Zhao Hui Lin Xiumei Wang Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles Scientific Reports Nanoparticles Epigenetic Histone modification Ion release Intracellular accumulation |
| title | Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles |
| title_full | Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles |
| title_fullStr | Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles |
| title_full_unstemmed | Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles |
| title_short | Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles |
| title_sort | histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles |
| topic | Nanoparticles Epigenetic Histone modification Ion release Intracellular accumulation |
| url | https://doi.org/10.1038/s41598-025-07206-z |
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