Genomic Analysis Methods of Microorganisms
Molecular approaches used to identify bacterial species use 16S rRNA and MLST to determine the genetic linkage of bacteria; MLST characterizes clonal linkages by examining differences in various gene loci. MLVA determines the genetic relationships of bacterial strains and biovar-level differences an...
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2025-01-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | https://agrifoodscience.com/index.php/TURJAF/article/view/7011 |
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| _version_ | 1832086504704311296 |
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| author | Canan Kebabçıoğlu Osman Erganiş |
| author_facet | Canan Kebabçıoğlu Osman Erganiş |
| author_sort | Canan Kebabçıoğlu |
| collection | DOAJ |
| description | Molecular approaches used to identify bacterial species use 16S rRNA and MLST to determine the genetic linkage of bacteria; MLST characterizes clonal linkages by examining differences in various gene loci. MLVA determines the genetic relationships of bacterial strains and biovar-level differences and assesses the copy number of repeated DNA sequences. Sequencing provides genetic data by identifying DNA sequences; Sanger sequencing is the basis for next-generation approaches. CRISPR modifies the genetic code and can correct mutations or control genes using Cas9. These methods are important for identifying bacterial species and annotating genomic information. The methods used for this purpose are brought together in this study. The explanation and detailed description of the methods examined will contribute to their use in the field of microbiology. |
| format | Article |
| id | doaj-art-5d16a819fa274ed28409fcfdc10b8f69 |
| institution | Kabale University |
| issn | 2148-127X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-5d16a819fa274ed28409fcfdc10b8f692025-02-06T13:44:57ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2025-01-0113123724310.24925/turjaf.v13i1.237-243.70115712Genomic Analysis Methods of MicroorganismsCanan Kebabçıoğlu0https://orcid.org/0000-0001-7299-9923Osman Erganiş1https://orcid.org/0000-0002-9340-9360Department of Microbiology, Faculty of Veterinary Medicine, Selçuk University, KONYA, TÜRKİYEDepartment of Microbiology, Faculty of Veterinary Medicine, Selçuk University, KONYA, TÜRKİYEMolecular approaches used to identify bacterial species use 16S rRNA and MLST to determine the genetic linkage of bacteria; MLST characterizes clonal linkages by examining differences in various gene loci. MLVA determines the genetic relationships of bacterial strains and biovar-level differences and assesses the copy number of repeated DNA sequences. Sequencing provides genetic data by identifying DNA sequences; Sanger sequencing is the basis for next-generation approaches. CRISPR modifies the genetic code and can correct mutations or control genes using Cas9. These methods are important for identifying bacterial species and annotating genomic information. The methods used for this purpose are brought together in this study. The explanation and detailed description of the methods examined will contribute to their use in the field of microbiology.https://agrifoodscience.com/index.php/TURJAF/article/view/7011bacteriaidentificationmolecular method |
| spellingShingle | Canan Kebabçıoğlu Osman Erganiş Genomic Analysis Methods of Microorganisms Turkish Journal of Agriculture: Food Science and Technology bacteria identification molecular method |
| title | Genomic Analysis Methods of Microorganisms |
| title_full | Genomic Analysis Methods of Microorganisms |
| title_fullStr | Genomic Analysis Methods of Microorganisms |
| title_full_unstemmed | Genomic Analysis Methods of Microorganisms |
| title_short | Genomic Analysis Methods of Microorganisms |
| title_sort | genomic analysis methods of microorganisms |
| topic | bacteria identification molecular method |
| url | https://agrifoodscience.com/index.php/TURJAF/article/view/7011 |
| work_keys_str_mv | AT canankebabcıoglu genomicanalysismethodsofmicroorganisms AT osmanerganis genomicanalysismethodsofmicroorganisms |