Detailed analysis of the African green monkey model of Nipah virus disease.
Henipaviruses are implicated in severe and frequently fatal pneumonia and encephalitis in humans. There are no approved vaccines or treatments available for human use, and testing of candidates requires the use of well-characterized animal models that mimic human disease. We performed a comprehensiv...
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| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117817&type=printable |
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| author | Sara C Johnston Thomas Briese Todd M Bell William D Pratt Joshua D Shamblin Heather L Esham Ginger C Donnelly Joshua C Johnson Lisa E Hensley W Ian Lipkin Anna N Honko |
| author_facet | Sara C Johnston Thomas Briese Todd M Bell William D Pratt Joshua D Shamblin Heather L Esham Ginger C Donnelly Joshua C Johnson Lisa E Hensley W Ian Lipkin Anna N Honko |
| author_sort | Sara C Johnston |
| collection | DOAJ |
| description | Henipaviruses are implicated in severe and frequently fatal pneumonia and encephalitis in humans. There are no approved vaccines or treatments available for human use, and testing of candidates requires the use of well-characterized animal models that mimic human disease. We performed a comprehensive and statistically-powered evaluation of the African green monkey model to define parameters critical to disease progression and the extent to which they correlate with human disease. African green monkeys were inoculated by the intratracheal route with 2.5 × 10(4) plaque forming units of the Malaysia strain of Nipah virus. Physiological data captured using telemetry implants and assessed in conjunction with clinical pathology were consistent with shock, and histopathology confirmed widespread tissue involvement associated with systemic vasculitis in animals that succumbed to acute disease. In addition, relapse encephalitis was identified in 100% of animals that survived beyond the acute disease phase. Our data suggest that disease progression in the African green monkey is comparable to the variable outcome of Nipah virus infection in humans. |
| format | Article |
| id | doaj-art-5cf7bccbf4d64207a9450613f3a2a433 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5cf7bccbf4d64207a9450613f3a2a4332025-08-20T02:15:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011781710.1371/journal.pone.0117817Detailed analysis of the African green monkey model of Nipah virus disease.Sara C JohnstonThomas BrieseTodd M BellWilliam D PrattJoshua D ShamblinHeather L EshamGinger C DonnellyJoshua C JohnsonLisa E HensleyW Ian LipkinAnna N HonkoHenipaviruses are implicated in severe and frequently fatal pneumonia and encephalitis in humans. There are no approved vaccines or treatments available for human use, and testing of candidates requires the use of well-characterized animal models that mimic human disease. We performed a comprehensive and statistically-powered evaluation of the African green monkey model to define parameters critical to disease progression and the extent to which they correlate with human disease. African green monkeys were inoculated by the intratracheal route with 2.5 × 10(4) plaque forming units of the Malaysia strain of Nipah virus. Physiological data captured using telemetry implants and assessed in conjunction with clinical pathology were consistent with shock, and histopathology confirmed widespread tissue involvement associated with systemic vasculitis in animals that succumbed to acute disease. In addition, relapse encephalitis was identified in 100% of animals that survived beyond the acute disease phase. Our data suggest that disease progression in the African green monkey is comparable to the variable outcome of Nipah virus infection in humans.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117817&type=printable |
| spellingShingle | Sara C Johnston Thomas Briese Todd M Bell William D Pratt Joshua D Shamblin Heather L Esham Ginger C Donnelly Joshua C Johnson Lisa E Hensley W Ian Lipkin Anna N Honko Detailed analysis of the African green monkey model of Nipah virus disease. PLoS ONE |
| title | Detailed analysis of the African green monkey model of Nipah virus disease. |
| title_full | Detailed analysis of the African green monkey model of Nipah virus disease. |
| title_fullStr | Detailed analysis of the African green monkey model of Nipah virus disease. |
| title_full_unstemmed | Detailed analysis of the African green monkey model of Nipah virus disease. |
| title_short | Detailed analysis of the African green monkey model of Nipah virus disease. |
| title_sort | detailed analysis of the african green monkey model of nipah virus disease |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117817&type=printable |
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