Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis

Abstract Environmental ‘light’ has a vital role in regulating plant growth and development. Transcriptomic profiling has been widely used to examine how light regulates mRNA levels on a genome‐wide scale, but the global role of translational regulation in the response to light is unknown. Through a...

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Main Authors: Ming‐Jung Liu, Szu‐Hsien Wu, Ho‐Ming Chen, Shu‐Hsing Wu
Format: Article
Language:English
Published: Springer Nature 2012-01-01
Series:Molecular Systems Biology
Subjects:
Online Access:https://doi.org/10.1038/msb.2011.97
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author Ming‐Jung Liu
Szu‐Hsien Wu
Ho‐Ming Chen
Shu‐Hsing Wu
author_facet Ming‐Jung Liu
Szu‐Hsien Wu
Ho‐Ming Chen
Shu‐Hsing Wu
author_sort Ming‐Jung Liu
collection DOAJ
description Abstract Environmental ‘light’ has a vital role in regulating plant growth and development. Transcriptomic profiling has been widely used to examine how light regulates mRNA levels on a genome‐wide scale, but the global role of translational regulation in the response to light is unknown. Through a transcriptomic comparison of steady‐state and polysome‐bound mRNAs, we reveal a clear impact of translational control on thousands of genes, in addition to transcriptomic changes, during photomorphogenesis. Genes encoding ribosomal protein are preferentially regulated at the translational level, which possibly contributes to the enhanced translation efficiency. We also reveal that mRNAs regulated at the translational level share characteristics of longer half‐lives and shorter cDNA length, and that transcripts with a cis‐element, TAGGGTTT, in their 5′ untranslated region have higher translatability. We report a previously neglected aspect of gene expression regulation during Arabidopsis photomorphogenesis. The identities and molecular signatures associated with mRNAs regulated at the translational level also offer new directions for mechanistic studies of light‐triggered translational enhancement in Arabidopsis.
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publishDate 2012-01-01
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spelling doaj-art-5ceea74a796b4a79a829bbd4aef9a9d32025-08-20T04:02:44ZengSpringer NatureMolecular Systems Biology1744-42922012-01-018111410.1038/msb.2011.97Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesisMing‐Jung Liu0Szu‐Hsien Wu1Ho‐Ming Chen2Shu‐Hsing Wu3Institute of Plant and Microbial Biology, Academia SinicaInstitute of Plant and Microbial Biology, Academia SinicaInstitute of Plant and Microbial Biology, Academia SinicaInstitute of Plant and Microbial Biology, Academia SinicaAbstract Environmental ‘light’ has a vital role in regulating plant growth and development. Transcriptomic profiling has been widely used to examine how light regulates mRNA levels on a genome‐wide scale, but the global role of translational regulation in the response to light is unknown. Through a transcriptomic comparison of steady‐state and polysome‐bound mRNAs, we reveal a clear impact of translational control on thousands of genes, in addition to transcriptomic changes, during photomorphogenesis. Genes encoding ribosomal protein are preferentially regulated at the translational level, which possibly contributes to the enhanced translation efficiency. We also reveal that mRNAs regulated at the translational level share characteristics of longer half‐lives and shorter cDNA length, and that transcripts with a cis‐element, TAGGGTTT, in their 5′ untranslated region have higher translatability. We report a previously neglected aspect of gene expression regulation during Arabidopsis photomorphogenesis. The identities and molecular signatures associated with mRNAs regulated at the translational level also offer new directions for mechanistic studies of light‐triggered translational enhancement in Arabidopsis.https://doi.org/10.1038/msb.2011.97photomorphogenesispolysometranslation
spellingShingle Ming‐Jung Liu
Szu‐Hsien Wu
Ho‐Ming Chen
Shu‐Hsing Wu
Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis
Molecular Systems Biology
photomorphogenesis
polysome
translation
title Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis
title_full Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis
title_fullStr Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis
title_full_unstemmed Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis
title_short Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis
title_sort widespread translational control contributes to the regulation of arabidopsis photomorphogenesis
topic photomorphogenesis
polysome
translation
url https://doi.org/10.1038/msb.2011.97
work_keys_str_mv AT mingjungliu widespreadtranslationalcontrolcontributestotheregulationofarabidopsisphotomorphogenesis
AT szuhsienwu widespreadtranslationalcontrolcontributestotheregulationofarabidopsisphotomorphogenesis
AT homingchen widespreadtranslationalcontrolcontributestotheregulationofarabidopsisphotomorphogenesis
AT shuhsingwu widespreadtranslationalcontrolcontributestotheregulationofarabidopsisphotomorphogenesis